PhD position in zebrafish lymphatic development
Sort on published: 2018-06-11
Uppsala University is a comprehensive research-intensive university with a strong international standing. Our mission is to pursue top-quality research and education and to interact constructively with society. Our most important assets are all the individuals whose curiosity and dedication make Uppsala University one of Sweden’s most exciting workplaces. Uppsala University has 42,000 students, 7,000 employees and a turnover of SEK 6.7 billion.
The Department of Immunology, Genetics and Pathology
at Uppsala University (www.igp.uu.se) has a broad research profile with strong research groups focused on cancer, autoimmune and genetic diseases. A fundamental idea at the department is to stimulate translational research and thereby closer interactions between medical research and health care. Research is presently conducted in the following areas: medical and clinical genetics, clinical immunology, pathology, neuro-oncology, vascular biology, radiation science and molecular tools. Department activities are also integrated with the units for Oncology, Clinical Genetics, Clinical Immunology, Clinical Pathology, and Hospital Physics at Akademiska sjukhuset, Uppsala. The department has teaching assignments in several education programmes, including Master Programmes, at the Faculty of Medicine, and in a number of educations at the Disciplinary Domain of Science and Technology. The department has a yearly turnover of around SEK 400 million, out of which more than half is made up of external funding. The staff amounts to approximately 340 employees, out of which 100 are PhD-students, and there are in total more than 600 affiliated people.
The student will be placed in the Lymphangiogenesis Lab research group lead by Kaska Koltowska (koltowskalab.com). Our research group studies lymphatic vessel formation in zebrafish, as a main model system. Lymphatic vessels are predominantly derived from a defined venous, vascular network, posing an intruding question of how cells know to form one vasculature from another. The overarching goal of my lab is to broaden our understanding of how lymphatic vessels form and establish function; and apply this knowledge to the development of new therapeutics.
Lymphangiogenesis includes the sprouting, migration and elaboration of new lymphatics from pre-existing vessels in both development and disease. The apical signalling pathway in lymphangiogenesis is the VEGFC/VEGFR3 pathway, yet how signalling controls cellular transcriptional output remains unknown. We used a forward genetic screen in zebrafish to identify the transcription factor mafba as essential for lymphatic vessel development. We found that mafba is required cell autonomously for the migration of lymphatic precursors after their initial sprouting from the posterior cardinal vein.
The main aim of this project is to characterise the migratory powerhouse downstream of mafba essential for lymphangiogenesis. In this project, the student will generate CRISPR mutants and characterise their role in lymphatic development.
Main techniques and analysis required in this project: RNA sequencing, CRISPR-Cas9 gRNAs technology, generation and maintenance of zebrafish lines, confocal fluorescence microscopy, immunohistochemistry, in situ hybridisation, objective quantification of imaging data, quality control, large data sets and statistical analysis, manuscripts preparation.
The student will receive a thorough education in molecular biology, zebrafish husbandry, fluorescence microscopy and handling of large datasets.
The successful candidate will devote most of the time towards his/her research level education. Other service activities within the department, e.g. education and administrative work can be included within the framework of the employment (maximum 20%). The position will be extended with the time devoted to teaching to allow four years of full time graduate studies.
Requirements of the applicant:
Applications are accepted from highly motivated candidates with a master degree in Biomedicine, (Molecular) Medicine, Molecular (Cell) Biology or similar. Written and oral proficiency in English is a prerequisite, as is basic knowledge of, and skills in molecular biology. A successful candidate should be a highly motivated, organized, reliable team player that can also work independently. Prior knowledge and experience in fluorescence microscopy, bioinformatics, programming, zebrafish husbandry and/or image-based analyses are a bonus.
The application should include:
1) a cover letter describing yourself, your research interests and your experience relevant to this position.
2) a CV/resume; a verified list of course grades; and contact details for at least two reference persons.
3) 2 page synopsis of the most recent discoveries in zebrafish lymphatic development (including reference list).
4) If available, a Master thesis (completed or in draft form) and letters of recommendation can also be included.
The application should be written in English as we are an international laboratory using English as a main language
Rules governing Ph.D. candidates are set out in the Higher Education Ordinance Chapter 5, §§ 1-7 and in Uppsala university's rules and guidelines http://regler.uu.se/?languageId=1
Uppsala University strives to be an inclusive workplace that promotes equal opportunities and attracts qualified candidates who can contribute to the University's excellence and diversity. We welcome applications from all sections of the community and from people of all backgrounds.
Information about education at the postgraduate level, admission requirements and admission decisions can be found at http://www2.medfarm.uu.se/utbildning/forskarniva/vill_du_borja/
Selection of applicants will be done by the future tutor for the selected student in consultation with the postgraduate study group of the Department. The Postgraduate Program Committee at the Disciplinary Domain of Medicine will formally approve the student's admission.
Salary: According to local agreement for PhD students and teaching assistants.
Starting date: According to the agreement.
Type of position: Full time position.
For further information about the position please Contact:
Kaska Koltowska, PhD, firstname.lastname@example.org
You are welcome to submit your application no later than 2018-07-10, UFV-PA 2018/2226.
Are you considering moving to Sweden to work at Uppsala University? If so, you will find a lot of information about working and living in Sweden at www.uu.se/joinus. You are also welcome to contact International Faculty and Staff Services at mailto:email@example.com.
We decline offers of recruitment and advertising help. We only accept the application the way described in the advertisement.
Placement: Department of Immunology, Genetics and Pathology
Type of employment: Full time , Temporary position longer than 6 months
Pay: Fixed pay
Number of positions: 1
Working hours: 100%
County: Uppsala län
Per Sundman, Saco-rådet
Ellena Papaioannou, Seko 018-471 3315
Suzanne Borén Andersson, TCO/ST 018-471 6251
Number of reference: UFV-PA 2018/2226
Last application date: 2018-07-10