On completion of the course the student shall be able to:
describe theoretical models for separation, optimisation and detection for chromatographic (liquid - and gas chromatography) and capillary electrophoretic methods and predict how changes in experimental conditions influence separation with these methods
describe the principle and the design of the components included in separation and mass spectrometric instrumentation
account for which type of information that can be obtained from a chromatogram, electropherogram and mass spectrum and carry out evaluation of simple spectra
choose appropriate technique regarding both separation and detection and choose and justify the choice of instrumentation based on the performance and requirements demanded by the applications
plan and carry out experiments that permit validation of the performance of methods such as sensitivity and selectivity
plan and carry out both qualitative and quantitative analysis of for example bioanalytical nature by means of the above mentioned methods
Liquid chromatography (reversed phase, normal phase, ion pair, ion chromatography, size-exclusion), gas chromatography and capillary electrophoresis (capillary zone electrophoresis, capillary electrochromatography, capillary gel electrophoresis). Theoretical models for separation, optimisation, and detection. Instrumentation for chromatography and capillary electrophoresis. Overview of systems to generate and measure vacuum. Sample introduction, ion sources and ionisation principles (electron ionisation, chemical ionisation, laser-induced desorption, chemical and photon ionisation at atmospheric pressure and electrospray). Overview of mass analysers mainly quadrupole, ion trap, time-of-flight and hybrid instruments. Detectors. Information that can be received from a chromatogram, electropherogram and mass spectrum. General aspects of interpretation of spectra generated with electron ionisation, chemical ionisation, electrospray and laser desorption. Connection of separation methods (gas chromatography, liquid chromatography and capillary electrophoresis) to mass spectrometry. Data management. Strategies for both relative and absolute quantification. Analytical applications based on chromatographic and capillary electrophoretic methods.
Laboratory projects elucidating gas - and liquid chromatography and capillary electrophoresis and laboratory work with mass spectrometry coupled to separation methods. Oral and written presentation of results.
The teaching is given as lectures, lessons, seminars and laboratory work. Course introduction, seminars and laboratory work are compulsory parts.
Written examination during the course (4.5 credits) and at the end of the course (4.5 credits). For passed grade on the course, it is required that the laboratory work has been presented and accepted. Laboratory work corresponds to 6 credits. The final grade corresponds to a weighted average of the results of the written examinations and the laboratory work.
week 01, 2015
Hoffmann, Edmond de;
Mass spectrometry : principles and applications