Upon completion of the course, the student shall be able to:
use knowledge in high resolving mass spectrometry (HRMS) with focus on analytical chemistry to solve analytical questions in the field of biology, veterinary and human medicine (molecular diagnostics).
motivate the choice of suitable combinations of sample preparation, chromatographic or electrophoretic separation and MS detection in order to solve analytical problems.
apply multivariate data analysis and bioinformatic tools for solving analytical problems.
explain the role of MS as an analytical tool for solving biological questions with different principles of imaging.
perform experimental work with the most essential separation and analytical methods for biology and molecular diagnostics as well as account for and present achieved results in a scientific format.
Overview of modern instrumentation for high resolution mass spectrometry including Fourier-transform ion cyclotron resonance mass spectrometry (FTICR), Orbitrap MS and Imaging MS. Emphasis is placed on the conditions and opportunities offered for different combinations of separation techniques, imaging techniques and mass spectrometry, with both qualitative and quantitative analytical aspects. Computational tools related to the questions are addressed.
The teaching is given as lectures, excecises, group discussion, experimental work, demonstrations, seminars and communication training (oral and written).
Written examination (7,5 hp). The laboratory experiments and presentations (oral and written) represents 7,5 hp. The final grade corresponds to a weighted average of the written examination and the laboratory results.
Laboratory work and communication training are mandatory. This course cannot be included in a degree together with the course 1KB158 Advanced Mass Spectrometry, 10.0 credits
week 30, 2017
Mass spectrometry : instrumentation, interpretation, and applications