The student should after completed course be able to
analyse structure/function relationships in biocatalysed reactions
predict possible catalytic mechanisms of given reaction types
present strategies for the analysis of kinetic mechanisms of catalysed reactions
account for industrial applications of biocatalysis
Reaction kinetics of simple and complex reactions (rate equations, reaction order, molecularity, rate limiting step). Thermodynamic aspects of reactions (reaction coordinates, activated complexes and transition states). Enzyme kinetics (steadystate kinetics, presteadystate kinetics). Reaction mechanisms (ligand binding; catalytic groups: acid/base, nucleophiles, electrophiles, cofactors, metals and entropic effects). Experimental analysis of catalytic and kinetic mechanisms (spectrometry, Xray crystallography, stopped flow, isotope effects, structure/reactivity relationships). Mathematical data analysis (regression analysis, model discrimination). Modelling of enzymatic reactions. Stereo chemistry (mechanisms). Structure/function relationships (protein folding, mutagenesis, enzyme inhibition, evolution of catalytic mechanism, catalytic antibodies). Catalytic nucleic acids (ribozymes, catalytic DNA).
The course includes lectures, groups discussions, tutorials and laboratory practicals. Participation in discussions, tutorials and laboratory practicals is mandatory. In addition, a mandatory literature project should be carried out.
Written exam (8 credits). Mandatory course components, tutorials, laboratory practicals and literature project which is presented orally and in writing corresponds to 7 points. The final grade is weighted from the results on the written exam and the practical part.
The course can not be included in higher education qualification together with 1KB463 Enzymology and Bioorganic Catalysis, 15 cr.
week 30, 2017
Enzymatic Reaction Mechanisms