The aim of this course is advanced knowledge about protein production on different scales, especially large-scale production, in the biotechnology industry. After completing this course the student should be able to:
Describe from start to finish the entire process of of protein production on different scales, including large-scale industrial production, that fulfils the quality requirements from both industry and regulations from governmental agencies (GLP,GMP)
Use a bioreactor in different ways to produce proteins
Perform of protein purification with regard to molecular properties and demands placed on product purity
Design purification methods for protiens with respect to starting material and production scale
Master and rationally select different methods for characterisation of proteins, with consideration to product requirements
Critically examine large scale industrial protein production processesand present the results orally.
Cell culturing bioreactors. Preparative scale protein purification and analysis . Protein characterisation by modern bioanalytical tools. GLP (Good Laboratory Practice) and GMP (Good Manufacturing Practice). Laws and regulations.Examination of large scale industrial protein production processes. Site visit at a biotechnology company.
Instructions are in the form of lectures, seminars, laboratory practicals and a group assignment with oral presentations. Communication training within selected profile areas is integrated with the course components.
A written test at the end of the course, and/or during the course, corresponding to 5 credits. Laboratory practicals and demonstrations, oral and written presentations of laboratory reports, and oral presentationof the group assignment t correspond to 5 credits in total. The final grade represents a weighted total of these components.
Cannot be counted towards the degree together with 1MB572 Protein biotechnology I, 1MB573 Protein biotechnology II, 1KB761 Protein biotechnology and 1KB762 Protein biotechnology.
The reading list is missing. For further information, please contact the responsible department.