Master’s studies

Syllabus for Enzymology and Bioorganic Catalysis

Enzymologi och bioorganisk katalys

Syllabus

  • 15 credits
  • Course code: 1KB463
  • Education cycle: Second cycle
  • Main field(s) of study and in-depth level: Chemistry A1N
  • Grading system: Fail (U), 3, 4, 5.
  • Established: 2010-03-18
  • Established by: The Faculty Board of Science and Technology
  • Applies from: week 34, 2010
  • Entry requirements: 120 credits in Science including 60 credits Chemistry including 15 credits Organic Chemistry or Biochemistry, or equivalent.
  • Responsible department: Department of Chemistry - BMC

Learning outcomes

The student should after completed course be able


  • to analyse structure/function relationships in biocatalysed reactions

  • to predict possible catalytic mechnisms of given reaction types

  • to present strategies for the analysis of kinetic mechanisms of catalysed reactions

  • to account for industrial applications of biocatalysis

Content

Reaction kinetics of simple and complex reactions (rate equations, reaction order, molecularity, rate limiting step). Thermodynamic aspects of reactions (reaction coordinates, activated complexes and transition states). Enzyme kinetics (steady-state kinetics, pre-steady-state kinetics). Reaction mechanisms (ligand binding; catalytic groups: acid/base, nucleophiles, electrophiles, co-factors, metals and entropic effects). Experimental analysis of catalytic and kinetic mechanisms (spectrometry, X-ray crystallography, stopped flow, isotope effects, structure/reactivity relationships). Mathematical data analysis (regression analysis, model discrimination). Modelling of enzymatic reactions. Stereo chemistry (mechanisms). Structure/function relationships (protein folding, mutagenesis, enzyme inhibition, evolution av catalytic mechanism, catalytic antibodies). Catalytic nucleic acids (ribozymes, catalytic DNA).

Instruction

The course includes lectures, groups discussions, tutorials and laboratory practicals. Participation in discussions, tutorials and laboratory practicals is mandatory. In addition, a mandatory literature project should be carried out, which is presented orally and in writing

Assessment

A written exam is held at the end of the course and corresponds to 9 credits. Mandatory course components, tutorials, laboratory practicals and literature project, corresponds to 6 points. For a final passing grade, reports from the mandatory components must be passed. The final grade is weighted from the results on the written exam and the practical part

Reading list

Applies from: week 35, 2010

  • Frey,, P.A. Hegeman,, A.D. Enzymatic Reaction Mechanisms

    Oxford University Press, 2007

    Find in the library