Syllabus for Advanced Mass Spectrometry

Avancerad masspektrometri


  • 15 credits
  • Course code: 1KB159
  • Education cycle: Second cycle
  • Main field(s) of study and in-depth level: Chemistry A1F

    Explanation of codes

    The code indicates the education cycle and in-depth level of the course in relation to other courses within the same main field of study according to the requirements for general degrees:

    First cycle
    G1N: has only upper-secondary level entry requirements
    G1F: has less than 60 credits in first-cycle course/s as entry requirements
    G1E: contains specially designed degree project for Higher Education Diploma
    G2F: has at least 60 credits in first-cycle course/s as entry requirements
    G2E: has at least 60 credits in first-cycle course/s as entry requirements, contains degree project for Bachelor of Arts/Bachelor of Science
    GXX: in-depth level of the course cannot be classified.

    Second cycle
    A1N: has only first-cycle course/s as entry requirements
    A1F: has second-cycle course/s as entry requirements
    A1E: contains degree project for Master of Arts/Master of Science (60 credits)
    A2E: contains degree project for Master of Arts/Master of Science (120 credits)
    AXX: in-depth level of the course cannot be classified.

  • Grading system: Fail (U), Pass (3), Pass with credit (4), Pass with distinction (5)
  • Established: 2017-03-09
  • Established by:
  • Revised: 2018-08-30
  • Revised by: The Faculty Board of Science and Technology
  • Applies from: week 24, 2019
  • Entry requirements: 120 credits with 60 credits in chemistry including Separation and Mass Spectrometry, 15 credits.
    English language proficiency that corresponds to English studies at upper secondary (high school) level in Sweden ("English 6").
  • Responsible department: Department of Chemistry - BMC

Learning outcomes

On completion of the course, the student should be able to:

  • use knowledge in high resolving mass spectrometry (HRMS) with focus on analytical chemistry to solve analytical questions in the field of biology, veterinary and human medicine (molecular diagnostics).
  • motivate the choice of suitable combinations of sample preparation, chromatographic or electrophoretic separation and MS detection in order to solve analytical problems.
  • apply multivariate data analysis and bioinformatic tools for solving analytical problems.
  • explain the role of MS as an analytical tool for solving biological questions with different principles of imaging.
  • perform experimental work with the most essential separation and analytical methods for biology and molecular diagnostics as well as account for and present achieved results in a scientific format.


Overview of modern instrumentation for high resolution mass spectrometry including Fourier-transform ion cyclotron resonance mass spectrometry (FTICR), Orbitrap MS and Imaging MS. Emphasis is placed on the conditions and opportunities offered for different combinations of separation techniques, imaging techniques and mass spectrometry, with both qualitative and quantitative analytical aspects. Computational tools related to the questions are addressed.


The teaching is given as lectures, excecises, group discussion, experimental work, demonstrations, seminars and communication training (oral and written).


Written examination (7,5 hp). The laboratory experiments and presentations (oral and written) represents 7,5 hp. The final grade corresponds to a weighted average of the written examination and the laboratory results.

If there are special reasons for doing so, an examiner may make an exception from the method of assessment indicated and allow a student to be assessed by another method. An example of special reasons might be a certificate regarding special pedagogical support from the disability coordinator of the university.

Other directives

Laboratory work and communication training are mandatory. This course cannot be included in a degree together with the course 1KB158 Advanced Mass Spectrometry, 10.0 credits

Syllabus Revisions

Reading list

Reading list

Applies from: week 24, 2019

  • Ekman, Rolf Mass spectrometry : instrumentation, interpretation, and applications

    Hoboken, N.J.: John Wiley & Sons, c2009

    Find in the library

  • Hoffmann, Edmond de; Stroobant, Vincent Mass spectrometry : principles and applications

    3. ed.: Chichester: Wiley, cop 2007

    Find in the library