Syllabus for Protein Biotechnology

Proteinbioteknologi

  • 10 credits
  • Course code: 1KB768
  • Education cycle: Second cycle
  • Main field(s) of study and in-depth level: Chemistry A1N, Technology A1N, Applied Biotechnology A1N
  • Grading system: Fail (U), Pass (3), Pass with credit (4), Pass with distinction (5)
  • Established: 2017-03-09
  • Established by:
  • Revised: 2018-08-30
  • Revised by: The Faculty Board of Science and Technology
  • Applies from: week 24, 2019
  • Entry requirements: 120 credits including Methods in Biotechnology. Technology of Process Modelling is recommended.
    English language proficiency that corresponds to English studies at upper secondary (high school) level in Sweden ("English 6").
  • Responsible department: Department of Chemistry - Ångström Laboratory

Learning outcomes

The aim of this course is advanced knowledge about protein production on different scales, especially large-scale production, in the biotechnology industry.
On completion of the course, the student should be able to:

  • Describe from start to finish the entire process of of protein production on different scales, including large-scale industrial production, that fulfils the quality requirements from both industry and regulations from governmental agencies (GLP,GMP)
  • Use a bioreactor in different ways to produce proteins
  • Perform of protein purification with regard to molecular properties and demands placed on product purity
  • Design purification methods for protiens with respect to starting material and production scale
  • Master and rationally select different methods for characterisation of proteins, with consideration to product requirements
  • Critically examine large scale industrial protein production processesand present the results orally.

Content

Cell culturing bioreactors. Preparative scale protein purification and analysis . Protein characterisation by modern bioanalytical tools. GLP (Good Laboratory Practice) and GMP (Good Manufacturing Practice). Laws and regulations.Examination of large scale industrial protein production processes. Site visit at a biotechnology company.

Instruction

Instructions are in the form of lectures, seminars, laboratory practicals and a group assignment with oral presentations. Communication training within selected profile areas is integrated with the course components.

Assessment

A written test at the end of the course, and/or during the course, corresponding to 5 credits. Laboratory practicals and demonstrations, oral and written presentations of laboratory reports, and oral presentationof the group assignment t correspond to 5 credits in total. The final grade represents a weighted total of these components.

If there are special reasons for doing so, an examiner may make an exception from the method of assessment indicated and allow a student to be assessed by another method. An example of special reasons might be a certificate regarding special pedagogical support from the disability coordinator of the university.

Other directives

Cannot be counted towards the degree together with 1MB572 Protein biotechnology I, 1MB573 Protein biotechnology II, 1KB761 Protein biotechnology and 1KB762 Protein biotechnology.

Reading list

The reading list is missing. For further information, please contact the responsible department.