During the course the students will confront a current, industry-related molecular biology/gene technology problem. The course objective is to give the students the in-depth understanding and skills necessary for independent problem-solving. Another objective is to show the steps necessary for commercialisation of a biotechnology invention.
After the course the student should be able to:
Analyse the structure of proteins with databases
Explain the classification and construction of proteins
Analyse and compare the amino acid sequences and structures of proteins and relate this information to function
Analyse and compare protein sequence data
Understand the function of individual amino acids and their influence on the solubility, structure, and function of proteins
Understand the major factors for protein folding
Construct bacterial expression plasmids for natural and modified genes
Analyse the DNA sequence of proteins for factors which can affect the expression and properties of the Construct primers for isolation of genes by PCR
Perform a PCR-based mutagenesis experiment
Purify proteins on a milligram scale by biochemical methods and perform a protein crystallisation experiment
Maintain a laboratory notebook according to GLP
Write a simple research proposal, business plan, and market analysis for a biotechnology invention
Lectures and computer-based exercises on biotechnology methods, and the structure, classification, and function of proteins. Project-based biotechnology experiments and the production of modified proteins.
Lectures, seminars, and computer-based exercises on entrepreneurship. Site-visit and other information on the demands that a biotechnologist can face professionally.
The student shall also present and defend solutions and results for a biotechnology problem, as well as document the laboratory part of the work in accordance with standards expected by future employers.
Course parts: Theory (8 ECTS), practical work (4 ECTS), Commercialisation (3 ECTS).
Instruction Instruction takes place in the form of lectures, seminars, and laboratory exercises. Participation is obligatory for the seminars, laboratory exercises, associated lectures, group exercises, exams, and computer exercises.
To demonstrate that the student has achieved the desired learning outcome, he or she must: Theory Course, (7 ECTS): have a passing grade on the written examination covering biotechnology and physical chemistry methods related to proteins, and protein structure and classification Practical Course: Present a correctly kept laboratory notebook (GLP standard), present the experimental results (5 ECTS) and a simple research plan with accompanying business plan and market analysis (3 ECTS).