Main field(s) of study and in-depth level:
Applied Biotechnology A1F,
Fail (U), Pass (3), Pass with credit (4), Pass with distinction (5)
The Faculty Board of Science and Technology
150 credits including (1) Molecular Cell Biology, or Functional Genomics, or Molecular Infection Biology, or Genome Biology, or (2) 30 credits within the Master Programme in Applied Biotechnology, the Master Programme in Molecular Biotechnology or Master Programme in Chemistry with Specialisation in Biochemistry.
During the course, the students work on a current molecular biological and gene technological problem. The aim is to provide advanced knowledge and skills that enable the students to find solutions on their own, and put these solutions into practice. On completion of the course, the student should be able to:
analyse the structure and construction of proteins by computer-based methods
describe the structure and classification of proteins
analyse and compare the amino acid sequence and structure of proteins, and relate this information to the function of proteins
outline the characteristics of individual amino acids and their effect on the solubility, structure and function of proteins
review factors significant for protein folding processes and stability
explain how proteins can be used for different industrial and academic purposes such as structure determination, organic synthesis and drug design
analyse the purity and stability of proteins and explain how to store them in the best way
describe how one can use biotechnical methods to construct plasmids for the expression of natural and modified genes
plan mutagenesis experiments to test protein stability and/or function
design primers to introduce mutations by means of PCR
carry out a PCR-based mutagenesis experiment
isolate proteins by biochemical methods
plan and carry out activity measurements of isolated proteins and characterise their purity and stability
keep a complete and informative lab journal, with an understanding of the requirements for GLP
design a simple research plan for a biotechnological invention
Lectures and computer-based exercises covering biotechnological methods and the structure and function of proteins. Lectures about industrial and other applications. Project-based biotechnological experiments,including production and analysis of modified proteins. The students will document their laboratory activities, which is aimed to prepare them for the demands of future employment. The student will also propose novel solutions to a chosen biotechnical problem.
The course consists of lectures, guest lectures, seminars, group work, computer and laboratory sessions. Participation in seminars, laboratory sessions and connected lectures, group work, tests and computer exercises is compulsory.
Modules: Theory (7 credits); Practicals (8 credits) The theory module is examined through written exams, as well as oral and written assignments (7 credits).
The practical module requires a correctly kept laboratory journal, and oral presentations of wet lab experiments (5 credits), as well asan oral presentation of an original research project (3 credits).
If there are special reasons for doing so, an examiner may make an exception from the method of assessment indicated and allow a student to be assessed by another method. An example of special reasons might be a certificate regarding special pedagogical support from the disability coordinator of the university.