Per Jemth
Professor at Department of Medical Biochemistry and Microbiology; Biochemistry and Cancer; Per Jemth
- Telephone:
- +46 70 425 04 36
- E-mail:
- Per.Jemth@imbim.uu.se
- Visiting address:
- BMC
Husargatan 3
752 37 UPPSALA - Postal address:
- Box 582
751 23 UPPSALA
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Short presentation
The ultimate goal of our research is to better understand fundamental structure-function and structure-reactivity relationships in proteins. Our research focuses on the nature, specificity and evolution of protein-ligand and protein-protein interactions, and also on protein folding, stability and allostery. We use protein engineering and biophysics to dissect the chemical reactions of proteins.
Keywords
- biophysics
- hpv
- human papillomavirus
- intrinsically disordered proteins
- kinetics
- molecular evolution
- protein
- protein folding
- protein-ligand interactions
Publications
Recent publications
- Gustavson syndrome is caused by an in-frame deletion in RBMX associated with potentially disturbed SH3 domain interactions (2024)
- Conservation of Affinity Rather Than Sequence Underlies a Dynamic Evolution of the Motif-Mediated p53/MDM2 Interaction in Ray-Finned Fishes (2024)
- Folded Alpha Helical Putative New Proteins from Apilactobacillus kunkeei (2024)
- Rescue of Escherichia coli auxotrophy by de novo small proteins (2023)
- Allostery Frustrates the Experimentalist (2023)
All publications
Articles
- Gustavson syndrome is caused by an in-frame deletion in RBMX associated with potentially disturbed SH3 domain interactions (2024)
- Conservation of Affinity Rather Than Sequence Underlies a Dynamic Evolution of the Motif-Mediated p53/MDM2 Interaction in Ray-Finned Fishes (2024)
- Folded Alpha Helical Putative New Proteins from Apilactobacillus kunkeei (2024)
- Rescue of Escherichia coli auxotrophy by de novo small proteins (2023)
- Allostery Frustrates the Experimentalist (2023)
- Intrinsically Disordered Flanking Regions Increase the Affinity of a Transcriptional Coactivator Interaction across Vertebrates (2023)
- Evaluation of affinity-purification coupled to mass spectrometry approaches for capture of short linear motif-based interactions (2023)
- Identification of motif-based interactions between SARS-CoV-2 protein domains and human peptide ligands pinpoint antiviral targets (2023)
- Large-scale phage-based screening reveals extensive pan-viral mimicry of host short linear motifs (2023)
- Evolution of affinity between p53 transactivation domain and MDM2 across the animal kingdom demonstrates high plasticity of motif-mediated interactions (2023)
- Proteome-scale mapping of binding sites in the unstructured regions of the human proteome (2022)
- Disordered Regions Flanking the Binding Interface Modulate Affinity between CBP and NCOA (2022)
- The dynamic properties of a nuclear coactivator binding domain are evolutionarily conserved (2022)
- Determinants of affinity, specificity, and phase separation in a supramodule from Post-synaptic density protein 95 (2022)
- A Syntenin Inhibitor Blocks Endosomal Entry of SARS-CoV-2 and a Panel of RNA Viruses (2022)
- Monoclonal antibodies binding data for SARS-CoV-2 proteins (2022)
- Development of Monoclonal Antibodies to Detect for SARS-CoV-2 Proteins (2022)
- Fuzziness and Frustration in the Energy Landscape of Protein Folding, Function, and Assembly (2021)
- CRISPR/Cas9-based inactivation of human papillomavirus oncogenes E6 or E7 induces senescence in cervical cancer cells (2021)
- Molecular Details of a Coupled Binding and Folding Reaction between the Amyloid Precursor Protein and a Folded Domain (2021)
- Large scale discovery of coronavirus-host factor protein interaction motifs reveals SARS-CoV-2 specific mechanisms and vulnerabilities (2021)
- Dissecting Inter-domain Cooperativity in the Folding of a Multi Domain Protein (2021)
- Divergent evolution of a protein-protein interaction revealed through ancestral sequence reconstruction and resurrection (2021)
- Double Mutant Cycles as a Tool to Address Folding, Binding, and Allostery (2021)
- An Early Association between the α-Helix of the TEAD Binding Domain of YAP and TEAD Drives the Formation of the YAP:TEAD Complex (2020)
- Structure and Characterization of Phosphoglucomutase 5 from Atlantic and Baltic Herring (2020)
- High affinity between CREBBP/p300 and NCOA evolved in vertebrates (2020)
- Mapping the transition state for a binding reaction between ancient intrinsically disordered proteins (2020)
- Functional interplay between protein domains in a supramodular structure involving the postsynaptic density protein PSD-95 (2020)
- Supertertiary protein structure affects an allosteric network (2020)
- Templated folding of intrinsically disordered proteins (2020)
- Affinity versus specificity in coupled binding and folding reactions (2019)
- Editorial overview (2019)
- Affinity and specificity of motif-based protein-protein interactions (2019)
- A structurally heterogeneous transition state underlies coupled binding and folding of disordered proteins (2019)
- Coupled Binding and Helix Formation Monitored by Synchrotron-Radiation Circular Dichroism (2019)
- Seeking allosteric networks in PDZ domains (2018)
- Structure and dynamics conspire in the evolution of affinity between intrinsically disordered proteins (2018)
- Probing Backbone Hydrogen Bonds in Proteins by Amide-to-Ester Mutations (2018)
- Binding Kinetics of the Intrinsically Disordered p53 Family Transactivation Domains and MDM2 (2018)
- Addressing the role of the alpha-helical extension in the folding of the third PDZ domain from PSD-95 (2017)
- How Fast Is Protein-Ligand Association? (2017)
- Emergence and evolution of an interaction between intrinsically disordered proteins (2017)
- The evolution of Sex-linked barring alleles in chickens involves both regulatory and coding changes in CDKN2A (2017)
- Understanding the role of phosphorylation in the binding mechanism of a PDZ domain (2017)
- Evolution of the p53-MDM2 pathway (2017)
- Activation Barrier-Limited Folding and Conformational Sampling of a Dynamic Protein Domain (2016)
- Coupled binding and folding of intrinsically disordered proteins (2016)
- Protein folding (2016)
- Improved affinity at the cost of decreased specificity (2016)
- Ligand binding to the PDZ domains of postsynaptic density protein 95 (2016)
- Binding Rate Constants Reveal Distinct Features of Disordered Protein Domains (2015)
- Rigidified Clicked Dimeric Ligands for Studying the Dynamics of the PDZ1-2 Supramodule of PSD-95 (2015)
- Design of a PDZbody, a bivalent binder of the E6 protein from human papillomavirus (2015)
- Deconvoluting Protein (Un)folding Structural Ensembles Using X-Ray Scattering, Nuclear Magnetic Resonance Spectroscopy and Molecular Dynamics Simulation (2015)
- Targeting Protein-Protein Interactions with Trimeric Ligands (2015)
- The binding mechanisms of intrinsically disordered proteins (2014)
- Only kinetics can prove conformational selection (2014)
- Nonequilibrium Capture Rates Induce Protein Accumulation and Enhanced Adsorption to Solid-State Nanopores (2014)
- Deciphering the mechanisms of binding induced folding at nearly atomic resolution (2014)
- Distinguishing induced fit from conformational selection (2014)
- Conserved nucleation sites reinforce the significance of phi analysis in proteinfolding studies (2014)
- Helical Propensity in an Intrinsically Disordered Protein Accelerates Ligand Binding (2014)
- A Frustrated Binding Interface for Intrinsically Disordered Proteins (2014)
- Thermodynamic Aspects of cAMP Dependent Protein Kinase Catalytic Subunit Allostery (2014)
- The Role of Backbone Hydrogen Bonds in the Transition State for Protein Folding of a PDZ Domain (2014)
- Probing backbone hydrogen bonding in PDZ/ligand interactions by protein amide-to-ester mutations (2014)
- The transition state structure for coupled binding and folding of disordered protein domains (2013)
- Probing the Role of Backbone Hydrogen Bonds in Protein-Peptide Interactions by Amide-to-Ester Mutations (2013)
- Single molecule unfolding and stretching of protein domains inside a solid-state nanopore by electric field (2013)
- Energetic pathway sampling in a protein interaction domain (2013)
- Single-Molecule Studies of Intrinsically Disordered Proteins Using Solid-State Nanopores (2013)
- Kinetics of Acrylodan-Labelled cAMP-Dependent Protein Kinase Catalytic Subunit Denaturation (2013)
- A high-affinity, dimeric inhibitor of PSD-95 bivalently interacts with PDZ1-2 and protects against ischemic brain damage (2012)
- Ligand binding by PDZ domains (2012)
- Interactions outside the boundaries of the canonical binding groove of a pdz domain influence ligand binding (2012)
- Fast Association and Slow Transitions in the Interaction between Two Intrinsically Disordered Protein Domains (2012)
- Characterization of the endopeptidase activity of tripeptidyl-peptidase II (2012)
- Folding pathways of proteins with increasing degree of sequence identities but different structure and function (2012)
- Side-Chain Interactions Form Late and Cooperatively in the Binding Reaction between Disordered Peptides and PDZ Domains (2012)
- An expanded view of the protein folding landscape of PDZ domains (2012)
- Tolerance of Protein Folding to a Circular Permutation in a PDZ Domain (2012)
- The Transition State of Coupled Folding and Binding for a Flexible beta-Finger (2012)
- Biophysical Characterization of the Complex between Human Papillomavirus E6 Protein and Synapse-associated Protein 97 (2011)
- Chemical, Thermal, and Electric Field Induced Unfolding of Single Protein Molecules Studied Using Nanopores (2011)
- Sequence-specific Long Range networks in PSD-95/Discs Large/ZO-1 (PDZ) Domains Tune Their Binding Selectivity (2011)
- Deciphering the kinetic binding mechanism of dimeric ligands, using a potent plasma-stable dimeric inhibitor of postsynaptic density protein-95 as an example (2010)
- Structural and functional characterization of CcmG from Pseudomonas aeruginosa, a key component of the bacterial cytochrome c maturation apparatus (2010)
- The plastic energy landscape of protein folding (2010)
- Design and synthesis of highly potent and plasma-stable dimeric inhibitors of the PSD-95-NMDA receptor interaction (2009)
- A sequential binding mechanism in a PDZ domain (2009)
- Distinguishing between smooth and rough free energy barriers in protein folding (2009)
- Investigating the structural plasticity of a cytochrome P450 (2009)
- Folding and stability of globular proteins and implications for function (2009)
- Modified peptides as potent inhibitors of the postsynaptic density-95/N-methyl-D-aspartate receptor interaction. (2008)
- Comparison of successive transition states for folding reveals alternative early folding pathways of two homologous proteins (2008)
- Reassessing a sparse energetic network within a single protein domain (2008)
- Demonstration by burst-phase analysis of a robust folding intermediate in the FF domain (2008)
- A conserved folding mechanism for PDZ domains (2007)
- A PDZ domain recapitulates a unifying mechanism for protein folding (2007)
- Identification and characterization of protein folding intermediates (2007)
- An on-pathway intermediate in the folding of a PDZ domain (2007)
- PDZ domains (2007)
- Two conserved residues govern the salt and pH dependencies of the binding reaction of a PDZ domain (2006)
- Demonstration of long-range interactions in a PDZ domain by NMR, kinetics, and protein engineering (2006)
- Renal cell carcinoma risk in type 2 von Hippel-Lindau disease correlates with defects in pVHL stability and HIF-1 alpha interactions (2006)
- The kinetics of PDZ domain-ligand interactions and implications for the binding mechanism (2005)
- The structure of the major transition state for folding of an FF domain from experiment and simulation. (2005)
- Fibroblast growth factors share binding sites in heparan sulphate. (2005)
- Simulation and experiment conspire to reveal cryptic intermediates and a slide from the nucleation-condensation to framework mechanism of folding. (2005)
- Demonstration of a low-energy on-pathway intermediate in a fast-folding protein by kinetics, protein engineering, and simulation. (2004)
- Processing of macromolecular heparin by heparanase (2003)
- Oligosaccharide library-based assessment of heparan sulfate6-O-sulfotransferase substrate specificity. (2003)
- Oligosaccharide Library-based Assessment of Heparan Sulfate 6-O-Sulfotransferase Substrate Specificity (2003)
- An ensemble of Theta class glutathione transferases with novel catalytic properties generated by stochastic recombination of fragments of two mammalian enzymes (2002)
- Biosynthetic oligosaccharide libraries for identification of protein-binding heparan sulfate motifs (2002)
- A semisyntetic glutathione peroxidase with high catalytic efficiency: Selenoglutathione transferase (2002)
- Role of the glutamyl alpha-carboxylate of the substrate glutathione in the catalytic mechanism of human glutathione transferase A1-1 (2001)
- The active-site residue Tyr-175 in human glyoxalase II contributes to binding of glutathione derivatives (2000)
- Conformational change and non-canonical interactions in the complex between human papillomavirus E6 protein and Synapse-Associated Protein 97
- Evaluation of affinity-purification coupled to mass spectrometry approaches for capture of short linear motif-based interactions
- Low-density lipoprotein receptor-related protein (LRP)-2/megalin plays a role as a receptor for retinol-binding protein on retina pigment epithelial cells of the eye.
- Dissecting inter-domain cooperativity in the folding of amulti domain protein
- Loss-of-affinity mutations and new interaction motifs underlie a dynamic evolution of the p53/MDM2 interaction in teleosts
- Analysis of AtZDP substrate specificity
- The 3'-DNA phosphatase AtZDP is present in a high molecular weight protein complex, able to repair gapped DNA with a 3' phosphate group
- Evolution of binding affinity between p53 and MDM2 across the animal kingdom
Chapters
