Facility for masspectrometry-based proteomics

Welcome to the mass spectrometry-based proteomics facility at Uppsala University. We are located at the Department of Chemistry - BMC, Analytical Chemistry. We offer tailored proteomic solutions for projects from both academia and industry.

Services

• Qualitative proteomics. Identification of proteins in complex biological samples.
• Quantitative proteomics. Label-free and labeling techniques.
• Body fluid proteomics, e.g. cerebrospinal fluid and plasma.
• Characterisation of post-translational modifications, e.g. protein phosphorylation and glycosylation.
• Proteomics of samples from non-human species, e.g. cats, dogs, plants, bacteria and parasites.
• Protein identification from solution and gel spots/bands.
• Analysis of proteins from tissue samples stored in biobanks (FFPE/OCT storage).
  
• Immunoprecipitation and identification of target proteins. The facility offers advice on experimental set up for identification of immunoprecipitated and co-immunoprecipitated proteins with MS.
  
• Protein extraction. Selective extraction of proteins from cells and tissues.
• Protein separation (1-D SDS-PAGE, Ion chromatography fractionation, SCX, ERLIC)
• Training services. We provide courses and workshops.
  

Equipment

• QExactive Plus Orbitrap MS (Thermo Scientific)
  
• Easy nanoLC 1000 och Easy nanoLC II (Thermo Scientific)
• 1260 Infinity LC (Agilent Technologies)

Applications

• Characterisation of truncated and modified proteins, elucidation of enzymatic cleavage sites.
• In-depth cerebrospinal fluid proteome analyses and determination of biomarkers for various disease states.
• Simultaneous extraction of membrane bound and cytosolic proteins from cells and tissues, followed by MS-analysis.
• Comprehensive characterisation of phosphoproteins and phosphoproteomes.