Chromatography and Capillary Electrophoresis
Syllabus, Master's level, 1KB150
This course has been discontinued.
- Code
- 1KB150
- Education cycle
- Second cycle
- Main field(s) of study and in-depth level
- Chemistry A1F
- Grading system
- Pass with distinction (5), Pass with credit (4), Pass (3), Fail (U)
- Finalised by
- The Faculty Board of Science and Technology, 15 March 2007
- Responsible department
- Department of Chemistry for Life Sciences
Entry requirements
Bachelor's degree and knowledge and achievements corresponding to course Spectrscopy, 10 credits and course in Analytical chemistry, 10 credits.
Learning outcomes
After completing the course the student should be able to
- describe and do calculations with theoretical models for liquid-liquid extraction
- describe theoretical models for separation, optimisation, and detection for chromatographic (liquid- and gas chromatography) and capillary electrophoretic methods
- predict from theoretic models how changes in experimental conditions will influence on separations with the methods above
- describe the construction and function of components in the instrumentation that is used for the methods above
- choose proper chromatographic or capillary electrophoretic method for a given problem
- plan and perform qualitative and quantitative analysis of for example bioanalytic character with the above mentioned methods
- plan and perform experiments to evaluate the quality of chromatographic methods
- present (English/Swedish) orally and in written reports experimentally obtained results
Content
Liquid-liquid-extraction of for example acids, bases and ion-pairs. Liquid chromatography (reversed phase, normal phase, ion-par, ion, size-exclusion), gas chromatography, and capillary electrophoresis (capillary zone electrophoresis, capillary electro chromatography, capillary gel electrophoresis). Theoretic models for separation, optimisation, and detection. Instrumentation for chromatography and capillary electrophoresis. Analytical applications of chromatographic and capillary electrophoretic methods.
Laboratory work in project form in gas and liquid chromatography and also capillary electrophoresis.
Instruction
Lectures, seminars, laboratory work. Compulsory moments: Course introduction, seminars and laboratory work.
Assessment
Examination is arranged during and/or at the end of the course and corresponds to 6 credits.. The laboratory work must also be passed and corresponds to 4 credits. The final grade is weighted.