Entry requirements

120 credits and knowledge and achievements corresponding to course Spectroscopy, 10 credits.

Learning outcomes

At the end of the course the student should:

- Be able to describe how biospecific interaction is used in modern biomedical analysis

- Be able to describe the most common sensor principles, such as electric, optical, and mechanic registration.

- Be able to compare critically different techniques with emphasis on sensitivity and selectivity.

- Be able to use biosensors.

Content

Lectures/seminars: Problem solving in analytical chemistry. Method validation. Protein/antibody-based sensors: protein immobilisation, specificity, binding constants, kinetics, diffusion. Electrochemical and optical sensors/transducers. Potentiometric methods. Redox-enzymes in amperometric methods. Conductimetric methods. Applications of the quartz microbalance. Optical methods: UV/Vis/IR, fluorescense, luminescence, fibre optics, surface plasmon resonance. Diagnostics and other biosensor applications are discussed critically with special emphasis on sensitivity, selectivity and stability.

Team work: Each group performs a critical evaluation and oral presentation of a chosen recent scientific biosensor article.

Laboratory lessons: Computer simulation. Surface plasmon resonance. Quartzmicrobalance.

Instruction

Lectures, lessons, laboratory work.

Assessment

Written exam at the end of the course (3 credits). Practical exercises, laboratory lessons and reportsare assigned 2 credits. The final grade is weighted.