Mass Spectrometry NV1
Syllabus, Master's level, 1KE988
This course has been discontinued.
- Code
- 1KE988
- Education cycle
- Second cycle
- Main field(s) of study and in-depth level
- Chemistry A1N
- Grading system
- Pass with distinction (5), Pass with credit (4), Pass (3), Fail (U)
- Finalised by
- The Faculty Board of Science and Technology, 4 November 2008
- Responsible department
- Department of Chemistry for Life Sciences
Entry requirements
120 credits with 60 credits Chemistry including 6 credits in Analytical Chemistry, or equivalent.
Learning outcomes
After completing the course the student should be able to:
- describe the principles for mass spectrometric instrumentation, such as vacuum, ion optics, mass analysers and detectors. Furthermore, choose and justify selection instrumentation considering instrument performance and application demands
- describe commonly used ionisation techniques and be able to choose and justify the selection of ionisation techniques for different applications
- have knowledge about and justify selection of separation techniques combined with mass spectrometric detection
- describe what types of information that can be obtained from a mass spectrum and have the ability to perform common spectra interpretation
- have knowledge about different aspects that has to be considered in quantitative analysis with mass spectrometric detection and be able to describe common strategies used in quantitative analysis
- suggest strategies for system optimisation regarding sensitivity and selectivity
Content
System for generating and measuring vacuum. Mass analysers especially quadrupole, ion trap, time-of-flight and hybrid instruments. Sample introduction, ion sources and ionisation principles (electron and chemical ionisation, laser induced desorption, chemical and photon ionisation performed at atmospheric pressure, and electrospray. Interfacing separation techniques (gas and liquid chromatography, capillary electrophoresis) to mass spectrometry. Information that can be obtained from a mass spectrum. General interpretation of spectra generated by electron and chemical ionisation, electrospray and laser desorption. Strategies for both relative and absolute quantification. Data handling.
Instruction
Lectures, laboratory work, seminars, and demonstrations. Compulsory moments: Course introduction, laboratory work and seminars.
Assessment
Examination is arranged during and/or at the end of the course. The laboratory work must also be passed. The laboratory work is evaluated to 3 credits. Students who have failed a test will be given new opportunities.