Advanced Mass Spectrometry
Syllabus, Master's level, 1KB158
This course has been discontinued.
- Code
- 1KB158
- Education cycle
- Second cycle
- Main field(s) of study and in-depth level
- Chemistry A1F
- Grading system
- Pass with distinction (5), Pass with credit (4), Pass (3), Fail (U)
- Finalised by
- The Faculty Board of Science and Technology, 10 March 2016
- Responsible department
- Department of Chemistry - BMC
Entry requirements
Separation and Mass Spectrometry, 15 credits, or Applied Analysis of Complex Samples, 15 credits
Learning outcomes
Upon completion of the course, the student shall be able to:
- use knowledge in high resolving mass spectrometry (HRMS) with focus on analytical chemistry to solve analytical questions in the field of biology, veterinary and human medicine (molecular diagnostics).
- motivate the choice of suitable combinations of sample preparation, chromatographic or electrophoretic separation and MS detection in order to solve analytical problems.
- apply multivariate data analysis and bioinformatic tools for solving analytical problems.
- explain the role of MS as an analytical tool for solving biological questions with different principles of imaging.
- perform experimental work with the most essential separation and analytical methods for biology and molecular diagnostics as well as account for and present achieved results in a scientific format.
Content
Overview of modern instrumentation for high resolution mass spectrometry including Fourier-transform ion cyclotron resonance mass spectrometry (FTICR), Orbitrap MS and Imaging MS. Emphasis is placed on the conditions and opportunities offered for different combinations of separation techniques, imaging techniques and mass spectrometry, with both qualitative and quantitative analytical aspects. Computational tools related to the questions are addressed.
Instruction
The teaching is given as lectures, excecises, group discussion, experimental work, demonstrations, seminars and communication training (oral and written).
Assessment
Written examination (5 hp). The laboratory experiments and presentations (oral and written) represents 5 hp. The final grade corresponds to a weighted average of the written examination and the laboratory results.
Other directives
Laboratory work and communication training are mandatory.