Entry requirements

Chemical Principles I/Basic chemistry, 10 credits, Chemical Principles II, 5 credits, the structure and function of Chemical substances, 10 credits and the chemistry of the Cell, 5 credits.

Learning outcomes

On completion of the course, the student should be able to:

- have understanding of different purification criteria at separation.

and be able to account for fundamental separation processes and their connection to molecular properties.

- have awareness about the most common separation and detection methods.

- account for application of different titrimetric, spectroscopic, electro-analytical and chromatographic methods regarding examination type, component analysis and concentration range.

- account for measurement quality at chemical determinations (method validation, quality control, data uncertainty) and apply relevant statistical methods on chemical measurement data.

- be able to choose and apply appropriate separation and detection methods on the basis of a simpler problem.

Content

Purification, isolation

Purificaton/crystallisation, the sublication, distillation

Separation

Molecular properties utilised during separation.

Phase distribution theory: extraction, adsorption, analytical - and preparative chromatography Precipitation/crystallisation.

Migration in an external field: electrophoresis, sedimentation/centrifugation.

Analysis

Issue - Sampling models for determination of both molecule - and element content in different sample types. - Sample preparation

Analytical aspects on common decision methods within titrimetry, the elektroanalysis, spectroscopy (atomic and molecular) and different chromatographic methods. Detection: spectroscopic methods (IR, UV-Vis, fluorescence, AA), light scattering, mass spectroscopy, electrochemical analysis

Experimental data processing

Calibration methods. Quality aspects on chemical methods of measurement. Statistical treatment of measurement data with help of different hypothesis testing (t-tests, F-tests and ANOVA and linear regression). Methods for determination of data uncertainty

Laboratory sessions

GC-HPLC, Preparative LC, extraction + AA, simple electrophoresis, computer exercises.

Instruction

The teaching is given as lectures, lessons, laboratory sessions, seminars and possible study visits. Course introduction, laboratory sessions, seminars and study visits are compulsory

Assessment

Written examination during or at the end of the course. For the course to be graded, is required that the laboratory work has been presented and accepted. Laboratory sessions that are part of the course correspond to 6 credits. The final grade corresponds to a joining of the results of the written examination and the laboratory sessions.

If there are special reasons for doing so, an examiner may make an exception from the method of assessment indicated and allow a student to be assessed by another method. An example of special reasons might be a certificate regarding special pedagogical support from the disability coordinator of the university.