Syllabus for Separation and Mass Spectrometry

Separation och masspektrometri

Syllabus

  • 15 credits
  • Course code: 1KB153
  • Education cycle: Second cycle
  • Main field(s) of study and in-depth level: Chemistry A1N

    Main field(s) of study and in-depth level

    The code indicates the education cycle and in-depth level of the course in relation to other courses within the same main field of study according to the requirements for general degrees:

    First cycle
    G1N: has only upper-secondary level entry requirements
    G1F: has less than 60 credits in first-cycle course/s as entry requirements
    G1E: contains specially designed degree project for Higher Education Diploma
    G2F: has at least 60 credits in first-cycle course/s as entry requirements
    G2E: has at least 60 credits in first-cycle course/s as entry requirements, contains degree project for Bachelor of Arts/Bachelor of Science
    GXX: in-depth level of the course cannot be classified.

    Second cycle
    A1N: has only first-cycle course/s as entry requirements
    A1F: has second-cycle course/s as entry requirements
    A1E: contains degree project for Master of Arts/Master of Science (60 credits)
    A2E: contains degree project for Master of Arts/Master of Science (120 credits)
    AXX: in-depth level of the course cannot be classified.

  • Grading system: Fail (U), Pass (3), Pass with credit (4), Pass with distinction (5)
  • Established: 2008-03-13
  • Established by:
  • Revised: 2018-08-30
  • Revised by: The Faculty Board of Science and Technology
  • Applies from: week 24, 2019
  • Entry requirements: 120 credits with 60 credits in chemistry including Analytical Chemistry, 10 credits.
    English language proficiency that corresponds to English studies at upper secondary (high school) level in Sweden ("English 6").
  • Responsible department: Department of Chemistry - BMC

Learning outcomes

On completion of the course, the student should be able to:

  • describe theoretical models for separation, optimisation and detection for chromatographic (liquid - and gas chromatography) and capillary electrophoretic methods and predict how changes in experimental conditions influence separation with these methods
  • describe the principle and the design of the components included in separation and mass spectrometric instrumentation
  • account for which type of information that can be obtained from a chromatogram, electropherogram and mass spectrum and carry out evaluation of simple spectra
  • choose appropriate technique regarding both separation and detection and choose and justify the choice of instrumentation based on the performance and requirements demanded by the applications
  • plan and carry out experiments that permit validation of the performance of methods such as sensitivity and selectivity
  • plan and carry out both qualitative and quantitative analysis of for example bioanalytical nature by means of the above mentioned methods

Content

Liquid chromatography (reversed phase, normal phase, ion pair, ion chromatography, size-exclusion), gas chromatography and capillary electrophoresis (capillary zone electrophoresis, capillary electrochromatography, capillary gel electrophoresis). Theoretical models for separation, optimisation, and detection. Instrumentation for chromatography and capillary electrophoresis. Overview of systems to generate and measure vacuum. Sample introduction, ion sources and ionisation principles (electron ionisation, chemical ionisation, laser-induced desorption, chemical and photon ionisation at atmospheric pressure and electrospray). Overview of mass analysers mainly quadrupole, ion trap, time-of-flight and hybrid instruments. Detectors. Information that can be received from a chromatogram, electropherogram and mass spectrum. General aspects of interpretation of spectra generated with electron ionisation, chemical ionisation, electrospray and laser desorption. Connection of separation methods (gas chromatography, liquid chromatography and capillary electrophoresis) to mass spectrometry. Data management. Strategies for both relative and absolute quantification. Analytical applications based on chromatographic and capillary electrophoretic methods.

Laboratory projects elucidating gas - and liquid chromatography and capillary electrophoresis and laboratory work with mass spectrometry coupled to separation methods. Oral and written presentation of results.

Instruction

The teaching is given as lectures, lessons, seminars and laboratory work. Course introduction, seminars and laboratory work are compulsory parts.

Assessment

Written examination during the course (4.5 credits) and at the end of the course (4.5 credits). For passed grade on the course, it is required that the laboratory work has been presented and accepted. Laboratory work corresponds to 6 credits. The final grade corresponds to a weighted average of the results of the written examinations and the laboratory work.

If there are special reasons for doing so, an examiner may make an exception from the method of assessment indicated and allow a student to be assessed by another method. An example of special reasons might be a certificate regarding special pedagogical support from the disability coordinator of the university.

Reading list

Reading list

Applies from: week 01, 2019