Douglas Huseby

Antibiotic class with potent in vivo activity targeting lipopolysaccharide synthesis in Gram-negative bacteria

Huseby, Douglas L.; Cao, Sha; Zamaratski, Edouard; Sooriyaarachchi, Sanjeewani et al.

Part of Proceedings of the National Academy of Sciences of the United States of America, 2024

• DOI for Antibiotic class with potent in vivo activity targeting lipopolysaccharide synthesis in Gram-negative bacteria
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The COMBINE pneumonia model: a multicenter study to standardize a mouse pneumonia model with Pseudomonas aeruginosa and Klebsiella pneumoniae for antibiotic development

Hansen, Jon U.; Arrazuria, Rakel; Hoover, Jennifer L.; Kerscher, Bernhard et al.

Part of Microbiology Spectrum, 2026

• DOI for The COMBINE pneumonia model: a multicenter study to standardize a mouse pneumonia model with Pseudomonas aeruginosa and Klebsiella pneumoniae for antibiotic development
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Integrated conjugative elements drive the formation of pandemic clones of Escherichia coli with hybrid chromosomes

Berruga Fernández, Talia; Huseby, Douglas L.; Koshla, Oksana; Shaukat, Anum et al.

Part of Molecular biology and evolution, 2026

• DOI for Integrated conjugative elements drive the formation of pandemic clones of Escherichia coli with hybrid chromosomes
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Antibiotic class with potent in vivo activity targeting lipopolysaccharide synthesis in Gram-negative bacteria

Huseby, Douglas L.; Cao, Sha; Zamaratski, Edouard; Sooriyaarachchi, Sanjeewani et al.

Part of Proceedings of the National Academy of Sciences of the United States of America, 2024

• DOI for Antibiotic class with potent in vivo activity targeting lipopolysaccharide synthesis in Gram-negative bacteria
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Apramycin efficacy against carbapenem- and aminoglycoside-resistant Escherichia coli and Klebsiella pneumoniae in murine bloodstream infection models

Frimodt-Møller, Niels; Hansen, Jon U.; Plattner, Michel; Huseby, Douglas L. et al.

Part of International Journal of Antimicrobial Agents, 2024

• DOI for Apramycin efficacy against carbapenem- and aminoglycoside-resistant Escherichia coli and Klebsiella pneumoniae in murine bloodstream infection models
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Discovery and Hit-to-Lead Optimization of Benzothiazole Scaffold- Based DNA Gyrase Inhibitors with Potent Activity against Acinetobacter baumannii and Pseudomonas aeruginosa

Cotman, Andrej Emanuel; Durcik, Martina; Tiz, Davide Benedetto; Fulgheri, Federica et al.

Part of Journal of Medicinal Chemistry, p. 1380-1425, 2023

• DOI for Discovery and Hit-to-Lead Optimization of Benzothiazole Scaffold- Based DNA Gyrase Inhibitors with Potent Activity against Acinetobacter baumannii and Pseudomonas aeruginosa
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The COMBINE pneumonia model: a multicenter study to standardize a mouse pneumonia model with Pseudomonas aeruginosa and Klebsiella pneumoniae for antibiotic development

Hansen, Jon U.; Arrazuria, Rakel; Hoover, Jennifer L.; Kerscher, Bernhard et al.

Part of Microbiology Spectrum, 2026

• DOI for The COMBINE pneumonia model: a multicenter study to standardize a mouse pneumonia model with Pseudomonas aeruginosa and Klebsiella pneumoniae for antibiotic development
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Integrated conjugative elements drive the formation of pandemic clones of Escherichia coli with hybrid chromosomes

Berruga Fernández, Talia; Huseby, Douglas L.; Koshla, Oksana; Shaukat, Anum et al.

Part of Molecular biology and evolution, 2026

• DOI for Integrated conjugative elements drive the formation of pandemic clones of Escherichia coli with hybrid chromosomes
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Antibiotic class with potent in vivo activity targeting lipopolysaccharide synthesis in Gram-negative bacteria

Huseby, Douglas L.; Cao, Sha; Zamaratski, Edouard; Sooriyaarachchi, Sanjeewani et al.

Part of Proceedings of the National Academy of Sciences of the United States of America, 2024

• DOI for Antibiotic class with potent in vivo activity targeting lipopolysaccharide synthesis in Gram-negative bacteria
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Apramycin efficacy against carbapenem- and aminoglycoside-resistant Escherichia coli and Klebsiella pneumoniae in murine bloodstream infection models

Frimodt-Møller, Niels; Hansen, Jon U.; Plattner, Michel; Huseby, Douglas L. et al.

Part of International Journal of Antimicrobial Agents, 2024

• DOI for Apramycin efficacy against carbapenem- and aminoglycoside-resistant Escherichia coli and Klebsiella pneumoniae in murine bloodstream infection models
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Discovery and Hit-to-Lead Optimization of Benzothiazole Scaffold- Based DNA Gyrase Inhibitors with Potent Activity against Acinetobacter baumannii and Pseudomonas aeruginosa

Cotman, Andrej Emanuel; Durcik, Martina; Tiz, Davide Benedetto; Fulgheri, Federica et al.

Part of Journal of Medicinal Chemistry, p. 1380-1425, 2023

• DOI for Discovery and Hit-to-Lead Optimization of Benzothiazole Scaffold- Based DNA Gyrase Inhibitors with Potent Activity against Acinetobacter baumannii and Pseudomonas aeruginosa
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New Dual Inhibitors of Bacterial Topoisomerases with Broad-Spectrum Antibacterial Activity and In Vivo Efficacy against Vancomycin-Intermediate Staphylococcus aureus

Durcik, Martina; Cotman, Andrej Emanuel; Toplak, Zan; Mozina, Stefan et al.

Part of Journal of Medicinal Chemistry, p. 3968-3994, 2023

• DOI for New Dual Inhibitors of Bacterial Topoisomerases with Broad-Spectrum Antibacterial Activity and In Vivo Efficacy against Vancomycin-Intermediate Staphylococcus aureus
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Positive Selection during Niche Adaptation Results in Large-Scale and Irreversible Rearrangement of Chromosomal Gene Order in Bacteria

Cao, Sha; Brandis, Gerrit; Huseby, Douglas L; Hughes, Diarmaid

Part of Molecular biology and evolution, 2022

• DOI for Positive Selection during Niche Adaptation Results in Large-Scale and Irreversible Rearrangement of Chromosomal Gene Order in Bacteria
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Evolution of Bacterial Interspecies Hybrids with Enlarged Chromosomes

Bartke, Katrin; Huseby, Douglas L; Brandis, Gerrit; Hughes, Diarmaid

Part of Genome Biology and Evolution, 2022

• DOI for Evolution of Bacterial Interspecies Hybrids with Enlarged Chromosomes
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Resistance/fitness trade-off is a barrier to the evolution of MarR inactivation mutants in Escherichia coli

Praski Alzrigat, Lisa; Huseby, Douglas L.; Brandis, Gerrit; Hughes, Diarmaid

Part of Journal of Antimicrobial Chemotherapy, p. 77-83, 2021

• DOI for Resistance/fitness trade-off is a barrier to the evolution of MarR inactivation mutants in Escherichia coli
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Phenotypic and genetic barriers to establishment of horizontally transferred genes encoding ribosomal protection proteins

Yadav, Kavita; Garoff, Linnea; Huseby, Douglas L; Hughes, Diarmaid

Part of Journal of Antimicrobial Chemotherapy, p. 1441-1447, 2021

• DOI for Phenotypic and genetic barriers to establishment of horizontally transferred genes encoding ribosomal protection proteins

Genetic Architecture and Fitness of Bacterial Interspecies Hybrids

Bartke, Katrin; Garoff, Linnéa; Huseby, Douglas L; Brandis, Gerrit et al.

Part of Molecular biology and evolution, p. 1472-1481, 2021

• DOI for Genetic Architecture and Fitness of Bacterial Interspecies Hybrids
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Antibacterial activity of apramycin at acidic pH warrants wide therapeutic window in the treatment of complicated urinary tract infections and acute pyelonephritis

Becker, Katja; Cao, Sha; Nilsson, Anna; Erlandsson, Maria et al.

Part of EBioMedicine, 2021

• DOI for Antibacterial activity of apramycin at acidic pH warrants wide therapeutic window in the treatment of complicated urinary tract infections and acute pyelonephritis
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Evaluating the potential efficacy and limitations of a phage for joint antibiotic and phage therapy of Staphylococcus aureus infections

Berryhill, Brandon A.; Huseby, Douglas L; McCall, Ingrid C.; Hughes, Diarmaid et al.

Part of Proceedings of the National Academy of Sciences of the United States of America, 2021

• DOI for Evaluating the potential efficacy and limitations of a phage for joint antibiotic and phage therapy of Staphylococcus aureus infections
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Mutant RNA polymerase can reduce susceptibility to antibiotics via ppGpp-independent induction of a stringent-like response

Brandis, Gerrit; Granström, Susanna; Leber, Anna T.; Bartke, Katrin et al.

Part of Journal of Antimicrobial Chemotherapy, p. 606-615, 2021

• DOI for Mutant RNA polymerase can reduce susceptibility to antibiotics via ppGpp-independent induction of a stringent-like response
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Population Bottlenecks Strongly Influence the Evolutionary Trajectory to Fluoroquinolone Resistance in Escherichia coli

Garoff, Linnéa; Pietsch, Franziska; Huseby, Douglas L; Lilja, Tua et al.

Part of Molecular biology and evolution, p. 1637-1646, 2020

• DOI for Population Bottlenecks Strongly Influence the Evolutionary Trajectory to Fluoroquinolone Resistance in Escherichia coli
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Structure-activity relationship studies on the inhibition of the bacterial translation of novel Odilorhabdins analogues

Loza, Einars; Sarciaux, Matthieu; Ikaunieks, Martins; Katkevics, Martins et al.

Part of Bioorganic & Medicinal Chemistry, 2020

• DOI for Structure-activity relationship studies on the inhibition of the bacterial translation of novel Odilorhabdins analogues

Antibiotic resistance by high-level intrinsic suppression of a frameshift mutation in an essential gene

Huseby, Douglas L; Brandis, Gerrit; Praski Alzrigat, Lisa; Hughes, Diarmaid

Part of Proceedings of the National Academy of Sciences of the United States of America, p. 3185-3191, 2020

• DOI for Antibiotic resistance by high-level intrinsic suppression of a frameshift mutation in an essential gene
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In vitro activity of apramycin against multidrug-, carbapenem- and aminoglycoside-resistant Enterobacteriaceae and Acinetobacter baumannii

Juhas, Mario; Widlake, Emma; Teo, Jeanette; Huseby, Douglas L et al.

Part of Journal of Antimicrobial Chemotherapy, p. 944-952, 2019

• DOI for In vitro activity of apramycin against multidrug-, carbapenem- and aminoglycoside-resistant Enterobacteriaceae and Acinetobacter baumannii
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Odilorhabdins, Antibacterial Agents that Cause Miscoding by Binding at a New Ribosomal Site

Pantel, Lucile; Florin, Tanja; Dobosz-Bartoszek, Malgorzata; Racine, Emilie et al.

Part of Molecular Cell, p. 83-94, 2018

• DOI for Odilorhabdins, Antibacterial Agents that Cause Miscoding by Binding at a New Ribosomal Site

Muramyl Endopeptidase Spr Contributes to Intrinsic Vancomycin Resistance in Salmonella enterica Serovar Typhimurium

Vestö, Kim; Huseby, Douglas L; Snygg, Lina; Wang, Helen et al.

Part of Frontiers in Microbiology, 2018

• DOI for Muramyl Endopeptidase Spr Contributes to Intrinsic Vancomycin Resistance in Salmonella enterica Serovar Typhimurium
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Methods to determine mutational trajectories after experimental evolution of antibiotic resistance.

Huseby, Douglas L; Hughes, Diarmaid

Part of Methods in Molecular Biology, p. 95-103, 2018

• DOI for Methods to determine mutational trajectories after experimental evolution of antibiotic resistance.

Effect of aminoacyl-tRNA synthetase mutations on susceptibility to ciprofloxacin in Escherichia coli

Garoff, Linnéa; Huseby, Douglas L; Praski Alzrigat, Lisa; Hughes, Diarmaid

Part of Journal of Antimicrobial Chemotherapy, p. 3285-3292, 2018

• DOI for Effect of aminoacyl-tRNA synthetase mutations on susceptibility to ciprofloxacin in Escherichia coli
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Imidazopyrazinones (IPYs): Non-Quinolone Bacterial Topoisomerase Inhibitors Showing Partial Cross-Resistance with Quinolones

Jeannot, Frédéric; Taillier, Thomas; Despeyroux, Pierre; Renard, Stéphane et al.

Part of Journal of Medicinal Chemistry, p. 3565-3581, 2018

• DOI for Imidazopyrazinones (IPYs): Non-Quinolone Bacterial Topoisomerase Inhibitors Showing Partial Cross-Resistance with Quinolones

Mutation supply and relative fitness shape the genotypes of ciprofloxacin-resistant Escherichia coli

Huseby, Douglas L.; Pietsch, Franziska; Brandis, Gerrit; Garoff, Linnéa et al.

Part of Molecular biology and evolution, p. 1029-1039, 2017

• DOI for Mutation supply and relative fitness shape the genotypes of ciprofloxacin-resistant Escherichia coli
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Early-Life Human Microbiota Associated With Childhood Allergy Promotes the T Helper 17 Axis in Mice

Petursdottir, Dagbjort H.; Nordlander, Sofia; Qazi, Khaleda Rahman; Carvalho-Queiroz, Claudia et al.

Part of Frontiers in Immunology, 2017

• DOI for Early-Life Human Microbiota Associated With Childhood Allergy Promotes the T Helper 17 Axis in Mice
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Ciprofloxacin selects for RNA polymerase mutations with pleiotropic antibiotic resistance effects

Pietsch, Franziska; Bergman, Jessica M.; Brandis, Gerrit; Marcusson, Linda L. et al.

Part of Journal of Antimicrobial Chemotherapy, p. 75-84, 2017

• DOI for Ciprofloxacin selects for RNA polymerase mutations with pleiotropic antibiotic resistance effects

Design, synthesis and in vitro biological evaluation of oligopeptides targeting E. coli type I signal peptidase (LepB)

De Rosa, Maria; Lu, Lu; Zamaratski, Edouard; Szałaj, Natalia et al.

Part of Bioorganic & Medicinal Chemistry, p. 897-911, 2017

• DOI for Design, synthesis and in vitro biological evaluation of oligopeptides targeting E. coli type I signal peptidase (LepB)
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Fitness cost constrains the spectrum of marR mutations in ciprofloxacin-resistant Escherichia coli: Multiple Antibiotic-Resistance, Gram-Negative Bacteria, Multidrug Efflux Pump, Urinary-Tract-Infections, Fluoroquinolone Resistance, Quinolone Resistance, Mechanisms, Expression, Sequence, Soxs

Praski Alzrigat, Lisa; Huseby, Douglas L; Brandis, Gerrit; Hughes, Diarmaid

Part of Journal of Antimicrobial Chemotherapy, p. 3016-3024, 2017

• DOI for Fitness cost constrains the spectrum of marR mutations in ciprofloxacin-resistant Escherichia coli: Multiple Antibiotic-Resistance, Gram-Negative Bacteria, Multidrug Efflux Pump, Urinary-Tract-Infections, Fluoroquinolone Resistance, Quinolone Resistance, Mechanisms, Expression, Sequence, Soxs
• Download full text (pdf) of Fitness cost constrains the spectrum of marR mutations in ciprofloxacin-resistant Escherichia coli: Multiple Antibiotic-Resistance, Gram-Negative Bacteria, Multidrug Efflux Pump, Urinary-Tract-Infections, Fluoroquinolone Resistance, Quinolone Resistance, Mechanisms, Expression, Sequence, Soxs

Having your cake and eating it - Staphylococcus aureus small colony variants can evolve faster growth rate without losing their antibiotic resistance

Brandis, Gerrit; Cao, Sha; Huseby, Douglas L.; Hughes, Diarmaid

Part of MICROBIAL CELL, p. 275-277, 2017

• DOI for Having your cake and eating it - Staphylococcus aureus small colony variants can evolve faster growth rate without losing their antibiotic resistance
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Alternative Evolutionary Pathways for Drug-Resistant Small Colony Variant Mutants in Staphylococcus aureus

Cao, Sha; Huseby, Douglas L; Brandis, Gerrit; Hughes, Diarmaid

Part of mBio, 2017

• DOI for Alternative Evolutionary Pathways for Drug-Resistant Small Colony Variant Mutants in Staphylococcus aureus
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The evolutionary maintenance of amino acid prototrophy in Escherichia coli

Lindahl, Otto; Berruga Fernández, Talia; Soekhoe, Jivanti; Huseby, Douglas L. et al.

Biological barriers to chromosomal hybrid formation in Escherichia coli

Berruga Fernández, Talia; Henriksson, Sofia; Borowiec, Karolina; Huseby, Douglas L. et al.

Clinical isolates of Klebsiella pneumoniae can transfer chromosomal DNA into Escherichia coli to create novel bacteria with stable hybrid chromosomes

Berruga Fernández, Talia; Kawaguchi, Jun; Huseby, Douglas L.; Hughes, Diarmaid