Westenhoffs grupp

Illustration som visar en utdragen filmrulle mot en bakgrund av växter. I varje bildruta finns en kristallstruktur av ett protein. I en av bildrutorna belyses proteinet med ljus vilket leder till att dess konformation ändras.

Tidsupplöst strukturbiologi av fotoaktiva proteiner

Målet med vår forskning är att förstå den strukturella dynamiken hos biologiska fotoaktiva system. Med hjälp av tidsupplöst kristallografi kan vi skapa molekylära filmer av övergången mellan systemens upplysta och icke-upplysta tillstånd. Det gör det möjligt att förstå hur konformationsförändringar i protein sker och identifiera vilka molekyler som är involverade i rörelsen. Genom att kombinera resultaten från våra olika forskningsområden kan vi öka förståelsen för hur fotoaktiva proteiner fungerar på en molekylär nivå.

Läs mer om vår forskning

Gruppmedlemmar

Forskningsledare: Sebastian Westenhoff

Gruppmedlemmar: Szabolcs Bódizs, Sadat Chowdhury, Anna-Lena Fischer, Candice Gautier, Lukas Grunewald, Taru Larkiala, Petra Mészáros, Leonardo Monrroy, Jennifer Mutisya, Kendra Njo, Sayan Prodhan, Yalin Zhou

Publikationer

Time-resolved serial crystallography to reveal protein structural changes

Mészáros, Petra; Westenhoff, Sebastian

Ingår i Trends in Biochemical Sciences (TIBS), s. 183-184, 2024
- DOI för Time-resolved serial crystallography to reveal protein structural changes
- Ladda ner fulltext (pdf) av Time-resolved serial crystallography to reveal protein structural changes
Directed ultrafast conformational changes accompany electron transfer in a photolyase as resolved by serial crystallography

Cellini, Andrea; Shankar, Madan Kumar; Nimmrich, Amke; Hunt, Leigh Anna et al.

Ingår i Nature Chemistry, s. 624-632, 2024
- DOI för Directed ultrafast conformational changes accompany electron transfer in a photolyase as resolved by serial crystallography
- Ladda ner fulltext (pdf) av Directed ultrafast conformational changes accompany electron transfer in a photolyase as resolved by serial crystallography
Cryo-EM structures of a bathy phytochrome histidine kinase reveal a unique light-dependent activation mechanism

Bódizs, Szabolcs; Mészáros, Petra; Grunewald, Lukas; Takala, Heikki et al.

Ingår i Structure, s. 1952-1962000, 2024
- DOI för Cryo-EM structures of a bathy phytochrome histidine kinase reveal a unique light-dependent activation mechanism
- Ladda ner fulltext (pdf) av Cryo-EM structures of a bathy phytochrome histidine kinase reveal a unique light-dependent activation mechanism
Microsecond time-resolved X-ray scattering by utilizing MHz repetition rate at second-generation XFELs

Konold, Patrick; Monrroy, Leonardo; Bellisario, Alfredo; de Matos Filipe, Diogo et al.

Ingår i Nature Methods, s. 1608-1611, 2024
- DOI för Microsecond time-resolved X-ray scattering by utilizing MHz repetition rate at second-generation XFELs
- Ladda ner fulltext (pdf) av Microsecond time-resolved X-ray scattering by utilizing MHz repetition rate at second-generation XFELs
Ground-state heterogeneity and vibrational energy redistribution in bacterial phytochrome observed with femtosecond 2D IR spectroscopy

Chenchiliyan, Manoop; Kuebel, Joachim; Ooi, Saik Ann; Salvadori, Giacomo et al.

Ingår i Journal of Chemical Physics, 2023
- DOI för Ground-state heterogeneity and vibrational energy redistribution in bacterial phytochrome observed with femtosecond 2D IR spectroscopy
- Ladda ner fulltext (pdf) av Ground-state heterogeneity and vibrational energy redistribution in bacterial phytochrome observed with femtosecond 2D IR spectroscopy
3D-printed sheet jet for stable megahertz liquid sample delivery at X-ray free-electron lasers

Konold, Patrick E.; You, Tong; Bielecki, Johan; Valerio, Joana et al.

Ingår i IUCrJ, s. 662-670, 2023
- DOI för 3D-printed sheet jet for stable megahertz liquid sample delivery at X-ray free-electron lasers
- Ladda ner fulltext (pdf) av 3D-printed sheet jet for stable megahertz liquid sample delivery at X-ray free-electron lasers
Structural mechanism of signal transduction in a phytochrome histidine kinase

Wahlgren, Weixiao Yuan; Claesson, Elin; Tuure, Iida; Trillo-Muyo, Sergio et al.

Ingår i Nature Communications, 2022
- DOI för Structural mechanism of signal transduction in a phytochrome histidine kinase
- Ladda ner fulltext (pdf) av Structural mechanism of signal transduction in a phytochrome histidine kinase
Structural basis of the radical pair state in photolyases and cryptochromes

Cellini, Andrea; Shankar, Madan Kumar; Wahlgren, Weixiao Yuan; Nimmrich, Amke et al.

Ingår i Chemical Communications, s. 4889-4892, 2022
- DOI för Structural basis of the radical pair state in photolyases and cryptochromes
- Ladda ner fulltext (pdf) av Structural basis of the radical pair state in photolyases and cryptochromes
Protein motions visualized by femtosecond time-resolved crystallography: The case of photosensory vs photosynthetic proteins

Westenhoff, Sebastian; Meszaros, Petra; Schmidt, Marius

Ingår i Current opinion in structural biology, 2022
- DOI för Protein motions visualized by femtosecond time-resolved crystallography: The case of photosensory vs photosynthetic proteins
- Ladda ner fulltext (pdf) av Protein motions visualized by femtosecond time-resolved crystallography: The case of photosensory vs photosynthetic proteins
Directed ultrafast conformational changes accompany electrontransfer in a photolyase as resolved by serial crystallography

Cellini, Andrea; Shankar, Madan Kumar; Nimmrich, Amke; Hunt, Leigh Anna et al.
Refolding of the Deinococcus Radiodurans phytochrome photosensory module and backbone resonance assignment by solution NMR

Vrhovac, Lidija; Levkovets, Maria; Orekhov, Vladislav; Westenhoff, Sebastian
Insight into signaling mechanism of the Deinococcus Radiodurans phytochrome chromophore binding core: a solution NMR study

Vrhovac, Lidija; Orekhov, Vladislav; Westenhoff, Sebastian