Mikael Engskog

Short presentation

I’m a medical doctor (bioanalytical chemistry) now working with metabolomics using both nuclear magnetic resonance and mass spectrometry as detection techniques.

Of particular focus is sample preparation strategies and analytical method development (data quality and reproducibility). I have experience working with plasma and cellular extracts.

Biography

I have a magisterexam (Biomolecular Chemistry, University collage of Södertörn) with a clear focus on organic chemistry and bioanalytical chemistry. The exam also cover the fields of pharmacology, biochemistry and physical chemistry.

My Ph D. was conducted at Karolinska Institutet, at the unit for analytical carbohydrate chemistry (Prof. Elke Schweda). The thesis deals with the structural characterization of lipopolysaccharides (Haemophilus influenzae) by methods such as masspectrometry, chromatography and NMR. The projects were done in collaboration with a group at Oxford University (Molecular Infectious Diseases Group and Department of Paediatrics). This experience has given me a wide understanding for the field of analytical chemistry when dealing with both practical labwork (sample preparations/purifications (with special focus on carbohydrates) and method development.

Technical skills:

• Analytical Chemistry/Organic Chemistry/Analytical Methodlogy
• Metabolomics (untargetted, cells and plasma)
• NMR (1H, 13C and 31P) 1D/2D
• Masspectrometry (GC-MS, ESI-MS, ESI-MSn, HPLC-ESI-MS, CE-ESI-MS/MS)
• HPAEC-PAD
• Chromatography (GC, HPLC and UPLC)
• Extraction techniques of lipopolysaccharides
• Sample purification
• Organic chemistry related modifications of carbohydrates
• Basic knowledge molecular biology related techniques (PCR, Western etc)

Publications:

A dual role for the lex2 locus: identification of galactosyltransferase activity in non-typeable Haemophilus influenzae strains 1124 and 2019.

Engskog MK, Yildirim HH, Li J, Richards JC, Deadman M, Hood DW, Schweda EK. Carbohydr Res. 2009 Mar 31;344(5):632-41. Epub 2009 Jan 17.

Lex2B, a phase-variable glycosyltransferase, adds either a glucose or a galactose to Haemophilus influenzae lipopolysaccharide.

Deadman ME, Hermant P, Engskog M, Makepeace K, Moxon ER, Schweda EK, Hood DW. Infect Immun. 2009 Jun;77(6):2376-84. Epub 2009 Mar 16.

Genes required for the synthesis of heptose-containing oligosaccharide outer core extensions in Haemophilus influenzae lipopolysaccharide.

Hood DW, Deadman ME, Engskog MK, Vitiazeva V, Makepeace K, Schweda EK, Moxon R. Microbiology. 2010 Nov;156(Pt 11):3421-31. Epub 2010 Aug 5.

Detailed structural features of lipopolysaccharide glycoforms in NTHi strain 2019.

Engskog MK, Mary Deadman, Jianjun Li, Derek W. Hood, Elke K. H. Schweda Carbohydr Res. 2011 Jul 15;346(10):1241-9. Epub 2011 Apr 28.

Nuclear magnetic resonance spectroscopy for analysis of intra-cellular metabolites.

Engskog MK, Haglöf J, Elmsjö A, Aftab O, Gustafsson M, Arvidsson T and Pettersson C. In manuscript (expected submission in summer 2012)

Structural analysis of the lipopolysaccharide from invasive non-typeable Haemophilusinfluenza strain R2866.

Engskog MK, Mary Deadman, Jianjun Li, Derek W. Hood, Elke K. H. Schweda. In manuscript (expected submission in summer 2012)

Acute toxic effects of BMAA in rodents: a metabolomics approach.

Elmsjö, A, Engskog MK, Haglöf J, Karlsson O, Brittebo E, Arvidsson T and Pettersson C. In manuscript (expected submission in autumn 2012)