Filipe Maia
Professor at Department of Cell and Molecular Biology; Molecular Biophysics
- Telephone:
- +46 18 471 46 42
- Mobile phone:
- +46 70 425 01 71
- E-mail:
- Filipe.Maia@icm.uu.se
- Visiting address:
- Husargatan 3
752 37 Uppsala - Postal address:
- Box 596
751 24 UPPSALA
Short presentation
Professor in molecular biophysics with specialization in coherent imaging with X-ray lasers. I develop lensless imaging methods, making use of X-ray free-electron lasers, to explore the wonderfully complex world of structural dynamics at the nanoscale. I am part of the Biophysics Network within the faculty. For more information check lmb.icm.uu.se.

Publications
Selection of publications
The role of transient resonances for ultra-fast imaging of single sucrose nanoclusters
Part of Nature Communications, 2020
- DOI for The role of transient resonances for ultra-fast imaging of single sucrose nanoclusters
- Download full text (pdf) of The role of transient resonances for ultra-fast imaging of single sucrose nanoclusters
Electrospray sample injection for single-particle imaging with x-ray lasers
Part of Science Advances, 2019
Femtosecond X-ray Fourier holography imaging of free-flying nanoparticles
Part of Nature Photonics, p. 150-153, 2018
Imaging single cells in a beam of live cyanobacteria with an X-ray laser
Part of Nature Communications, 2015
Chemical composition mapping with nanometre resolution by soft X-ray microscopy
Part of Nature Photonics, p. 765-769, 2014
High-throughput imaging of heterogeneous cell organelles with an X-ray laser
Part of Nature Photonics, p. 943-949, 2014
Shapes and vorticities of superfluid helium nanodroplets
Part of Science, p. 906-909, 2014
The Coherent X-ray Imaging Data Bank
Part of Nature Methods, p. 854-855, 2012
Single mimivirus particles intercepted and imaged with an X-ray laser
Part of Nature, p. 78-81, 2011
Femtosecond X-ray protein nanocrystallography
Part of Nature, p. 73-77, 2011
Recent publications
Ptychographic reconstructions performed in real time and offline have equivalent quality
Part of Scientific Reports, 2025
- DOI for Ptychographic reconstructions performed in real time and offline have equivalent quality
- Download full text (pdf) of Ptychographic reconstructions performed in real time and offline have equivalent quality
Aerosol size determination via light scattering of viruses and protein complexes
Part of Communications Physics, 2025
- DOI for Aerosol size determination via light scattering of viruses and protein complexes
- Download full text (pdf) of Aerosol size determination via light scattering of viruses and protein complexes
Observation of a single protein by ultrafast X-ray diffraction
Part of Light, 2024
- DOI for Observation of a single protein by ultrafast X-ray diffraction
- Download full text (pdf) of Observation of a single protein by ultrafast X-ray diffraction
Part of Nature Methods, p. 1608-1611, 2024
- DOI for Microsecond time-resolved X-ray scattering by utilizing MHz repetition rate at second-generation XFELs
- Download full text (pdf) of Microsecond time-resolved X-ray scattering by utilizing MHz repetition rate at second-generation XFELs
Part of Journal of Synchrotron Radiation, p. 222-232, 2024
- DOI for Enhancing electrospray ionization efficiency for particle transmission through an aerodynamic lens stack
- Download full text (pdf) of Enhancing electrospray ionization efficiency for particle transmission through an aerodynamic lens stack
All publications
Articles in journal
Ptychographic reconstructions performed in real time and offline have equivalent quality
Part of Scientific Reports, 2025
- DOI for Ptychographic reconstructions performed in real time and offline have equivalent quality
- Download full text (pdf) of Ptychographic reconstructions performed in real time and offline have equivalent quality
Aerosol size determination via light scattering of viruses and protein complexes
Part of Communications Physics, 2025
- DOI for Aerosol size determination via light scattering of viruses and protein complexes
- Download full text (pdf) of Aerosol size determination via light scattering of viruses and protein complexes
Observation of a single protein by ultrafast X-ray diffraction
Part of Light, 2024
- DOI for Observation of a single protein by ultrafast X-ray diffraction
- Download full text (pdf) of Observation of a single protein by ultrafast X-ray diffraction
Part of Nature Methods, p. 1608-1611, 2024
- DOI for Microsecond time-resolved X-ray scattering by utilizing MHz repetition rate at second-generation XFELs
- Download full text (pdf) of Microsecond time-resolved X-ray scattering by utilizing MHz repetition rate at second-generation XFELs
Part of Journal of Synchrotron Radiation, p. 222-232, 2024
- DOI for Enhancing electrospray ionization efficiency for particle transmission through an aerodynamic lens stack
- Download full text (pdf) of Enhancing electrospray ionization efficiency for particle transmission through an aerodynamic lens stack
Resolving Nonequilibrium Shape Variations among Millions of Gold Nanoparticles
Part of ACS Nano, p. 15576-15589, 2024
- DOI for Resolving Nonequilibrium Shape Variations among Millions of Gold Nanoparticles
- Download full text (pdf) of Resolving Nonequilibrium Shape Variations among Millions of Gold Nanoparticles
Helium-electrospray improves sample delivery in X-ray single-particle imaging experiments
Part of Scientific Reports, 2024
- DOI for Helium-electrospray improves sample delivery in X-ray single-particle imaging experiments
- Download full text (pdf) of Helium-electrospray improves sample delivery in X-ray single-particle imaging experiments
Part of Journal of the American Chemical Society, p. 19555-19565, 2024
- DOI for Controlling Drug Partitioning in Individual Protein Condensates through Laser-Induced Microscale Phase Transitions
- Download full text (pdf) of Controlling Drug Partitioning in Individual Protein Condensates through Laser-Induced Microscale Phase Transitions
3D-printed sheet jet for stable megahertz liquid sample delivery at X-ray free-electron lasers
Part of IUCrJ, p. 662-670, 2023
- DOI for 3D-printed sheet jet for stable megahertz liquid sample delivery at X-ray free-electron lasers
- Download full text (pdf) of 3D-printed sheet jet for stable megahertz liquid sample delivery at X-ray free-electron lasers
Part of IUCrJ, p. 204-214, 2022
- DOI for Unsupervised learning approaches to characterizing heterogeneous samples using X-ray single-particle imaging
- Download full text (pdf) of Unsupervised learning approaches to characterizing heterogeneous samples using X-ray single-particle imaging
Noise reduction and mask removal neural network for X-ray single-particle imaging
Part of Journal of applied crystallography, p. 122-132, 2022
- DOI for Noise reduction and mask removal neural network for X-ray single-particle imaging
- Download full text (pdf) of Noise reduction and mask removal neural network for X-ray single-particle imaging
Ptychographic wavefront characterization for single-particle imaging at x-ray lasers
Part of Optica, p. 551-562, 2021
- DOI for Ptychographic wavefront characterization for single-particle imaging at x-ray lasers
- Download full text (pdf) of Ptychographic wavefront characterization for single-particle imaging at x-ray lasers
Introduction to the special issue on Ptychography: software and technical developments
Part of Journal of applied crystallography, p. 384-385, 2021
3D diffractive imaging of nanoparticle ensembles using an x-ray laser
Part of Optica, p. 15-23, 2021
- DOI for 3D diffractive imaging of nanoparticle ensembles using an x-ray laser
- Download full text (pdf) of 3D diffractive imaging of nanoparticle ensembles using an x-ray laser
Pulse-to-pulse wavefront sensing at free-electron lasers using ptychography
Part of Journal of applied crystallography, p. 949-956, 2020
- DOI for Pulse-to-pulse wavefront sensing at free-electron lasers using ptychography
- Download full text (pdf) of Pulse-to-pulse wavefront sensing at free-electron lasers using ptychography
Acquired Functional Capsid Structures in Metazoan Totivirus-like dsRNA Virus
Part of Structure, p. 888-+, 2020
Megahertz single-particle imaging at the European XFEL
Part of Communications Physics, 2020
- DOI for Megahertz single-particle imaging at the European XFEL
- Download full text (pdf) of Megahertz single-particle imaging at the European XFEL
The role of transient resonances for ultra-fast imaging of single sucrose nanoclusters
Part of Nature Communications, 2020
- DOI for The role of transient resonances for ultra-fast imaging of single sucrose nanoclusters
- Download full text (pdf) of The role of transient resonances for ultra-fast imaging of single sucrose nanoclusters
Part of Structural Dynamics, 2020
- DOI for Perspectives on single particle imaging with x rays at the advent of high repetition rate x-ray free electron laser sources
- Download full text (pdf) of Perspectives on single particle imaging with x rays at the advent of high repetition rate x-ray free electron laser sources
Three-Dimensional Coherent Bragg Imaging of Rotating Nanoparticles
Part of Physical Review Letters, 2020
- DOI for Three-Dimensional Coherent Bragg Imaging of Rotating Nanoparticles
- Download full text (pdf) of Three-Dimensional Coherent Bragg Imaging of Rotating Nanoparticles
Part of Scientific Data, 2020
- DOI for Diffraction data from aerosolized Coliphage PR772 virus particles imaged with the Linac Coherent Light Source
- Download full text (pdf) of Diffraction data from aerosolized Coliphage PR772 virus particles imaged with the Linac Coherent Light Source
Electrospray sample injection for single-particle imaging with x-ray lasers
Part of Science Advances, 2019
Evaluation of serial crystallographic structure determination within megahertz pulse trains
Part of Structural Dynamics, 2019
- DOI for Evaluation of serial crystallographic structure determination within megahertz pulse trains
- Download full text (pdf) of Evaluation of serial crystallographic structure determination within megahertz pulse trains
Femtosecond X-ray Fourier holography imaging of free-flying nanoparticles
Part of Nature Photonics, p. 150-153, 2018
Part of IUCrJ, p. 531-541, 2018
A statistical approach to detect protein complexes at X-ray free electron laser facilities
Part of Communications Physics, 2018
Cryo-EM structure of a Marseilleviridae virus particle reveals a large internal microassembly
Part of Virology, p. 239-245, 2018
Three-dimensional localization of nanoscale battery reactions using soft X-ray tomography
Part of Nature Communications, 2018
- DOI for Three-dimensional localization of nanoscale battery reactions using soft X-ray tomography
- Download full text (pdf) of Three-dimensional localization of nanoscale battery reactions using soft X-ray tomography
Rayleigh-scattering microscopy for tracking and sizing nanoparticles in focused aerosol beams
Part of IUCrJ, p. 673-680, 2018
- DOI for Rayleigh-scattering microscopy for tracking and sizing nanoparticles in focused aerosol beams
- Download full text (pdf) of Rayleigh-scattering microscopy for tracking and sizing nanoparticles in focused aerosol beams
Megahertz serial crystallography
Part of Nature Communications, 2018
- DOI for Megahertz serial crystallography
- Download full text (pdf) of Megahertz serial crystallography
Single-particle imaging without symmetry constraints at an X-ray free-electron laser
Part of IUCrJ, p. 727-736, 2018
- DOI for Single-particle imaging without symmetry constraints at an X-ray free-electron laser
- Download full text (pdf) of Single-particle imaging without symmetry constraints at an X-ray free-electron laser
Part of Nature Scientific Data, 2018
Coherent soft X-ray diffraction imaging of Coliphage PR772 at the Linac coherent light source
Part of Scientific Data, 2017
Correlations in scattered X-ray laser pulses reveal nanoscale structural features of viruses
Part of Physical Review Letters, 2017
Part of Scientific Reports, 2017
- DOI for Structural variability and complexity of the giant Pithovirus sibericum particle revealed by high-voltage electron cryo-tomography and energy-filtered electron cryo-microscopy
- Download full text (pdf) of Structural variability and complexity of the giant Pithovirus sibericum particle revealed by high-voltage electron cryo-tomography and energy-filtered electron cryo-microscopy
Experimental strategies for imaging bioparticles with femtosecond hard X-ray pulses
Part of IUCrJ, p. 251-262, 2017
Nanosurveyor: a framework for real-time data processing
Part of Advanced Structural and Chemical Imaging, 2017
- DOI for Nanosurveyor: a framework for real-time data processing
- Download full text (pdf) of Nanosurveyor: a framework for real-time data processing
Near-edge X-ray refraction fine structure microscopy
Part of Applied Physics Letters, 2017
Part of Scientific Reports, 2016
- DOI for The infectious particle of insect-borne totivirus-like Omono River virus has raised ridges and lacks fibre complexes
- Download full text (pdf) of The infectious particle of insect-borne totivirus-like Omono River virus has raised ridges and lacks fibre complexes
Open data set of live cyanobacterial cells imaged using an X-ray laser
Part of Scientific Data, 2016
- DOI for Open data set of live cyanobacterial cells imaged using an X-ray laser
- Download full text (pdf) of Open data set of live cyanobacterial cells imaged using an X-ray laser
Part of Scientific Data, 2016
A data set from flash X-ray imaging of carboxysomes
Part of Scientific Data, 2016
- DOI for A data set from flash X-ray imaging of carboxysomes
- Download full text (pdf) of A data set from flash X-ray imaging of carboxysomes
Hummingbird: monitoring and analyzing flash X-ray imaging experiments in real time
Part of Journal of applied crystallography, p. 1042-1047, 2016
Single-shot diffraction data from the Mimivirus particle using an X-ray free-electron laser
Part of Scientific Data, 2016
- DOI for Single-shot diffraction data from the Mimivirus particle using an X-ray free-electron laser
- Download full text (pdf) of Single-shot diffraction data from the Mimivirus particle using an X-ray free-electron laser
Data publication with the structural biology data grid supports live analysis
Part of Nature Communications, 2016
- DOI for Data publication with the structural biology data grid supports live analysis
- Download full text (pdf) of Data publication with the structural biology data grid supports live analysis
CCP-FEL: a collection of computer programs for free-electron laser research
Part of Journal of applied crystallography, p. 1117-1120, 2016
The trickle before the torrent-diffraction data from X-ray lasers
Part of Scientific Data, 2016
- DOI for The trickle before the torrent-diffraction data from X-ray lasers
- Download full text (pdf) of The trickle before the torrent-diffraction data from X-ray lasers
SHARP: a distributed GPU-based ptychographic solver
Part of Journal of applied crystallography, p. 1245-1252, 2016
Three-dimensional reconstruction of the giant mimivirus particle with an X-ray free-electron laser
Part of Physical Review Letters, 2015
- DOI for Three-dimensional reconstruction of the giant mimivirus particle with an X-ray free-electron laser
- Download full text (pdf) of Three-dimensional reconstruction of the giant mimivirus particle with an X-ray free-electron laser
Imaging single cells in a beam of live cyanobacteria with an X-ray laser
Part of Nature Communications, 2015
The linac coherent light source single particle imaging road map
Part of Structural Dynamics, 2015
Part of Optics Express, p. 28914-28925, 2014
Chemical composition mapping with nanometre resolution by soft X-ray microscopy
Part of Nature Photonics, p. 765-769, 2014
Part of Journal of applied crystallography, p. 1118-1131, 2014
- DOI for Cheetah: software for high-throughput reduction and analysis of serial femtosecond X-ray diffraction data
- Download full text (pdf) of Cheetah: software for high-throughput reduction and analysis of serial femtosecond X-ray diffraction data
High-throughput imaging of heterogeneous cell organelles with an X-ray laser
Part of Nature Photonics, p. 943-949, 2014
Shapes and vorticities of superfluid helium nanodroplets
Part of Science, p. 906-909, 2014
Part of Optics Express, p. 28729-28742, 2013
Sensing the wavefront of x-ray free-electron lasers using aerosol spheres
Part of Optics Express, p. 12385-12394, 2013
The extraction of single-particle diffraction patterns from a multiple-particle diffraction pattern
Part of Optics Express, p. 15102-15112, 2013
Impact of wave front and coherence optimization in coherent diffractive imaging
Part of Optics Express, p. 11441-11447, 2013
Part of Journal of Physics B, p. 164033, 2013
Augmented projections for ptychographic imaging
Part of Inverse Problems, p. 115009, 2013
Time-resolved protein nanocrystallography using an X-ray free-electron laser
Part of Optics Express, p. 2706-2716, 2012
In vivo protein crystallization opens new routes in structural biology
Part of Nature Methods, p. 259-262, 2012
Lipidic phase membrane protein serial femtosecond crystallography
Part of Nature Methods, p. 263-265, 2012
The Coherent X-ray Imaging Data Bank
Part of Nature Methods, p. 854-855, 2012
Femtosecond free-electron laser x-ray diffraction data sets for algorithm development
Part of Optics Express, p. 4149-4158, 2012
Femtosecond dark-field imaging with an X-ray free electron laser
Part of Optics Express, p. 13501-13512, 2012
Noise-robust coherent diffractive imaging with a single diffraction pattern
Part of Optics Express, p. 16650-16661, 2012
Part of The international journal of high performance computing applications, p. 378-385, 2012
Self-terminating diffraction gates femtosecond X-ray nanocrystallography measurements
Part of Nature Photonics, p. 35-40, 2012
Fractal morphology, imaging and mass spectrometry of single aerosol particles in flight
Part of Nature, p. 513-517, 2012
Unsupervised classification of single-particle X-ray diffraction snapshots by spectral clustering
Part of Optics Express, p. 16542-16549, 2011
Unsupervised classification of single-particle X-ray diffraction snapshots by spectral clustering.
Part of Optics Express, p. 15929-15936, 2011
On the Feasibility of Nanocrystal Imaging Using Intense and Ultrashort X-ray Pulses
Part of ACS Nano, p. 139-146, 2011
Single mimivirus particles intercepted and imaged with an X-ray laser
Part of Nature, p. 78-81, 2011
Structure-factor analysis of femtosecond micro-diffraction patterns from protein nanocrystals
Part of Acta Crystallographica Section A, p. 131-140, 2011
Femtosecond X-ray protein nanocrystallography
Part of Nature, p. 73-77, 2011
Radiation damage in protein serial femtosecond crystallography using an x-ray free-electron laser
Part of Physical Review B. Condensed Matter and Materials Physics, 2011
Compressive auto-indexing in femtosecond nanocrystallography
Part of Ultramicroscopy, p. 807-811, 2011
Part of Physical Review Letters, 2010
Hawk: the image reconstruction package for coherent X-ray diffractive imaging
Part of Journal of applied crystallography, p. 1535-1539, 2010
Femtosecond diffractive imaging of biological cells
Part of Journal of Physics B, p. 194015, 2010
Single-Shot Diffractive Imaging with a Table-Top Femtosecond Soft X-Ray Laser-Harmonics Source
Part of Physical Review Letters, 2009
Part of Physical Review E. Statistical, Nonlinear, and Soft Matter Physics, 2009
Single particle X-ray diffractive imaging
Part of Nano letters (Print), p. 310-6, 2008
Femtosecond diffractive imaging with a soft-X-ray free-electron laser
Part of Nature Physics, p. 839-843, 2006
Interactive visualization of electron density slices
Part of J. Appl. Cryst., p. 563-565, 2005
Articles, review/survey
Condor: a simulation tool for flash X-ray imaging
Part of Journal of applied crystallography, p. 1356-1362, 2016
- DOI for Condor: a simulation tool for flash X-ray imaging
- Download full text (pdf) of Condor: a simulation tool for flash X-ray imaging
Structural studies of melting on the picosecond time scale
Part of Physical Chemistry, Chemical Physics - PCCP, p. 6344-6349, 2008
Feasibility of imaging living cells at subnanometer resolutions by ultrafast X-ray diffraction
Part of Quarterly reviews of biophysics (Print), p. 181-204, 2008
Comprehensive doctoral thesis
Conference papers
Ptychographic imaging for the characterization of X-ray free-electron laser beams
Part of X-RAY MICROSCOPY CONFERENCE 2016 (XRM 2016), 2017
- DOI for Ptychographic imaging for the characterization of X-ray free-electron laser beams
- Download full text (pdf) of Ptychographic imaging for the characterization of X-ray free-electron laser beams
Real-Time Data-Intensive Computing
Part of Proceedings Of The 12Th International Conference On Synchrotron Radiation Instrumentation (SRI2015), 2016
Profiling structured beams using injected aerosols
Part of Proceedings of SPIE, p. 850403, 2012