Filipe Maia
Professor at Department of Cell and Molecular Biology; Molecular Biophysics
- Telephone:
- +46 18 471 46 42
- Mobile phone:
- +46 70 425 01 71
- E-mail:
- Filipe.Maia@icm.uu.se
- Visiting address:
- Husargatan 3
752 37 Uppsala - Postal address:
- Box 596
751 24 UPPSALA
Visiting researcher at Department of Medicinal Chemistry
- Mobile phone:
- +46 70 425 01 71
- E-mail:
- filipe.maia@icm.uu.se
- Visiting address:
- Biomedicinskt Centrum BMC, Husargatan 3
- Postal address:
- Box 574
751 23 UPPSALA
Download contact information for Filipe Maia at Department of Medicinal Chemistry
Short presentation
Professor in molecular biophysics with specialization in coherent imaging with X-ray lasers. I develop lensless imaging methods, making use of X-ray free-electron lasers, to explore the wonderfully complex world of structural dynamics at the nanoscale. I am part of the Biophysics Network within the faculty. For more information check lmb.icm.uu.se.

Publications
Selection of publications
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The role of transient resonances for ultra-fast imaging of single sucrose nanoclusters
Part of Nature Communications, 2020
- DOI for The role of transient resonances for ultra-fast imaging of single sucrose nanoclusters
- Download full text (pdf) of The role of transient resonances for ultra-fast imaging of single sucrose nanoclusters
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Electrospray sample injection for single-particle imaging with x-ray lasers
Part of Science Advances, 2019
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Femtosecond X-ray Fourier holography imaging of free-flying nanoparticles
Part of Nature Photonics, p. 150-153, 2018
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Imaging single cells in a beam of live cyanobacteria with an X-ray laser
Part of Nature Communications, 2015
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High-throughput imaging of heterogeneous cell organelles with an X-ray laser
Part of Nature Photonics, p. 943-949, 2014
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Chemical composition mapping with nanometre resolution by soft X-ray microscopy
Part of Nature Photonics, p. 765-769, 2014
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Shapes and vorticities of superfluid helium nanodroplets
Part of Science, p. 906-909, 2014
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The Coherent X-ray Imaging Data Bank
Part of Nature Methods, p. 854-855, 2012
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Single mimivirus particles intercepted and imaged with an X-ray laser
Part of Nature, p. 78-81, 2011
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Femtosecond X-ray protein nanocrystallography
Part of Nature, p. 73-77, 2011
Recent publications
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2025
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Impact of gas background on XFEL single-particle imaging
Part of Scientific Reports, 2025
- DOI for Impact of gas background on XFEL single-particle imaging
- Download full text (pdf) of Impact of gas background on XFEL single-particle imaging
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Aerosol size determination via light scattering of viruses and protein complexes
Part of Communications Physics, 2025
- DOI for Aerosol size determination via light scattering of viruses and protein complexes
- Download full text (pdf) of Aerosol size determination via light scattering of viruses and protein complexes
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Advancing time-resolved structural biology: latest strategies in cryo-EM and X-ray crystallography
Part of Nature Methods, p. 1420-1435, 2025
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Part of Crystals, 2025
- DOI for Coarse-Graining and Classifying Massive High-Throughput XFEL Datasets of Crystallization in Supercooled Water
- Download full text (pdf) of Coarse-Graining and Classifying Massive High-Throughput XFEL Datasets of Crystallization in Supercooled Water
All publications
Articles in journal
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Impact of gas background on XFEL single-particle imaging
Part of Scientific Reports, 2025
- DOI for Impact of gas background on XFEL single-particle imaging
- Download full text (pdf) of Impact of gas background on XFEL single-particle imaging
-
Aerosol size determination via light scattering of viruses and protein complexes
Part of Communications Physics, 2025
- DOI for Aerosol size determination via light scattering of viruses and protein complexes
- Download full text (pdf) of Aerosol size determination via light scattering of viruses and protein complexes
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Part of Crystals, 2025
- DOI for Coarse-Graining and Classifying Massive High-Throughput XFEL Datasets of Crystallization in Supercooled Water
- Download full text (pdf) of Coarse-Graining and Classifying Massive High-Throughput XFEL Datasets of Crystallization in Supercooled Water
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Part of npj Computational Materials, 2025
- DOI for SPRING, an effective and reliable framework for image reconstruction in single-particle Coherent Diffraction Imaging
- Download full text (pdf) of SPRING, an effective and reliable framework for image reconstruction in single-particle Coherent Diffraction Imaging
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Part of Structure, p. 829-835, 2025
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Ptychographic reconstructions performed in real time and offline have equivalent quality
Part of Scientific Reports, 2025
- DOI for Ptychographic reconstructions performed in real time and offline have equivalent quality
- Download full text (pdf) of Ptychographic reconstructions performed in real time and offline have equivalent quality
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Part of Nature Methods, p. 1608-1611, 2024
- DOI for Microsecond time-resolved X-ray scattering by utilizing MHz repetition rate at second-generation XFELs
- Download full text (pdf) of Microsecond time-resolved X-ray scattering by utilizing MHz repetition rate at second-generation XFELs
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Observation of a single protein by ultrafast X-ray diffraction
Part of Light, 2024
- DOI for Observation of a single protein by ultrafast X-ray diffraction
- Download full text (pdf) of Observation of a single protein by ultrafast X-ray diffraction
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Part of Journal of Synchrotron Radiation, p. 222-232, 2024
- DOI for Enhancing electrospray ionization efficiency for particle transmission through an aerodynamic lens stack
- Download full text (pdf) of Enhancing electrospray ionization efficiency for particle transmission through an aerodynamic lens stack
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Resolving Nonequilibrium Shape Variations among Millions of Gold Nanoparticles
Part of ACS Nano, p. 15576-15589, 2024
- DOI for Resolving Nonequilibrium Shape Variations among Millions of Gold Nanoparticles
- Download full text (pdf) of Resolving Nonequilibrium Shape Variations among Millions of Gold Nanoparticles
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Helium-electrospray improves sample delivery in X-ray single-particle imaging experiments
Part of Scientific Reports, 2024
- DOI for Helium-electrospray improves sample delivery in X-ray single-particle imaging experiments
- Download full text (pdf) of Helium-electrospray improves sample delivery in X-ray single-particle imaging experiments
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Part of Journal of the American Chemical Society, p. 19555-19565, 2024
- DOI for Controlling Drug Partitioning in Individual Protein Condensates through Laser-Induced Microscale Phase Transitions
- Download full text (pdf) of Controlling Drug Partitioning in Individual Protein Condensates through Laser-Induced Microscale Phase Transitions
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3D-printed sheet jet for stable megahertz liquid sample delivery at X-ray free-electron lasers
Part of IUCrJ, p. 662-670, 2023
- DOI for 3D-printed sheet jet for stable megahertz liquid sample delivery at X-ray free-electron lasers
- Download full text (pdf) of 3D-printed sheet jet for stable megahertz liquid sample delivery at X-ray free-electron lasers
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Part of IUCrJ, p. 204-214, 2022
- DOI for Unsupervised learning approaches to characterizing heterogeneous samples using X-ray single-particle imaging
- Download full text (pdf) of Unsupervised learning approaches to characterizing heterogeneous samples using X-ray single-particle imaging
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Noise reduction and mask removal neural network for X-ray single-particle imaging
Part of Journal of applied crystallography, p. 122-132, 2022
- DOI for Noise reduction and mask removal neural network for X-ray single-particle imaging
- Download full text (pdf) of Noise reduction and mask removal neural network for X-ray single-particle imaging
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Ptychographic wavefront characterization for single-particle imaging at x-ray lasers
Part of Optica, p. 551-562, 2021
- DOI for Ptychographic wavefront characterization for single-particle imaging at x-ray lasers
- Download full text (pdf) of Ptychographic wavefront characterization for single-particle imaging at x-ray lasers
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Introduction to the special issue on Ptychography: software and technical developments
Part of Journal of applied crystallography, p. 384-385, 2021
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3D diffractive imaging of nanoparticle ensembles using an x-ray laser
Part of Optica, p. 15-23, 2021
- DOI for 3D diffractive imaging of nanoparticle ensembles using an x-ray laser
- Download full text (pdf) of 3D diffractive imaging of nanoparticle ensembles using an x-ray laser
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Pulse-to-pulse wavefront sensing at free-electron lasers using ptychography
Part of Journal of applied crystallography, p. 949-956, 2020
- DOI for Pulse-to-pulse wavefront sensing at free-electron lasers using ptychography
- Download full text (pdf) of Pulse-to-pulse wavefront sensing at free-electron lasers using ptychography
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Acquired Functional Capsid Structures in Metazoan Totivirus-like dsRNA Virus
Part of Structure, p. 888-+, 2020
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Megahertz single-particle imaging at the European XFEL
Part of Communications Physics, 2020
- DOI for Megahertz single-particle imaging at the European XFEL
- Download full text (pdf) of Megahertz single-particle imaging at the European XFEL
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The role of transient resonances for ultra-fast imaging of single sucrose nanoclusters
Part of Nature Communications, 2020
- DOI for The role of transient resonances for ultra-fast imaging of single sucrose nanoclusters
- Download full text (pdf) of The role of transient resonances for ultra-fast imaging of single sucrose nanoclusters
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Part of Structural Dynamics, 2020
- DOI for Perspectives on single particle imaging with x rays at the advent of high repetition rate x-ray free electron laser sources
- Download full text (pdf) of Perspectives on single particle imaging with x rays at the advent of high repetition rate x-ray free electron laser sources
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Three-Dimensional Coherent Bragg Imaging of Rotating Nanoparticles
Part of Physical Review Letters, 2020
- DOI for Three-Dimensional Coherent Bragg Imaging of Rotating Nanoparticles
- Download full text (pdf) of Three-Dimensional Coherent Bragg Imaging of Rotating Nanoparticles
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Part of Scientific Data, 2020
- DOI for Diffraction data from aerosolized Coliphage PR772 virus particles imaged with the Linac Coherent Light Source
- Download full text (pdf) of Diffraction data from aerosolized Coliphage PR772 virus particles imaged with the Linac Coherent Light Source
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Electrospray sample injection for single-particle imaging with x-ray lasers
Part of Science Advances, 2019
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Evaluation of serial crystallographic structure determination within megahertz pulse trains
Part of Structural Dynamics, 2019
- DOI for Evaluation of serial crystallographic structure determination within megahertz pulse trains
- Download full text (pdf) of Evaluation of serial crystallographic structure determination within megahertz pulse trains
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Femtosecond X-ray Fourier holography imaging of free-flying nanoparticles
Part of Nature Photonics, p. 150-153, 2018
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Part of IUCrJ, p. 531-541, 2018
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A statistical approach to detect protein complexes at X-ray free electron laser facilities
Part of Communications Physics, 2018
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Cryo-EM structure of a Marseilleviridae virus particle reveals a large internal microassembly
Part of Virology, p. 239-245, 2018
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Three-dimensional localization of nanoscale battery reactions using soft X-ray tomography
Part of Nature Communications, 2018
- DOI for Three-dimensional localization of nanoscale battery reactions using soft X-ray tomography
- Download full text (pdf) of Three-dimensional localization of nanoscale battery reactions using soft X-ray tomography
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Rayleigh-scattering microscopy for tracking and sizing nanoparticles in focused aerosol beams
Part of IUCrJ, p. 673-680, 2018
- DOI for Rayleigh-scattering microscopy for tracking and sizing nanoparticles in focused aerosol beams
- Download full text (pdf) of Rayleigh-scattering microscopy for tracking and sizing nanoparticles in focused aerosol beams
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Megahertz serial crystallography
Part of Nature Communications, 2018
- DOI for Megahertz serial crystallography
- Download full text (pdf) of Megahertz serial crystallography
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Single-particle imaging without symmetry constraints at an X-ray free-electron laser
Part of IUCrJ, p. 727-736, 2018
- DOI for Single-particle imaging without symmetry constraints at an X-ray free-electron laser
- Download full text (pdf) of Single-particle imaging without symmetry constraints at an X-ray free-electron laser
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Coherent soft X-ray diffraction imaging of Coliphage PR772 at the Linac coherent light source
Part of Scientific Data, 2017
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Correlations in scattered X-ray laser pulses reveal nanoscale structural features of viruses
Part of Physical Review Letters, 2017
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Part of Scientific Reports, 2017
- DOI for Structural variability and complexity of the giant Pithovirus sibericum particle revealed by high-voltage electron cryo-tomography and energy-filtered electron cryo-microscopy
- Download full text (pdf) of Structural variability and complexity of the giant Pithovirus sibericum particle revealed by high-voltage electron cryo-tomography and energy-filtered electron cryo-microscopy
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Experimental strategies for imaging bioparticles with femtosecond hard X-ray pulses
Part of IUCrJ, p. 251-262, 2017
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Nanosurveyor: a framework for real-time data processing
Part of Advanced Structural and Chemical Imaging, 2017
- DOI for Nanosurveyor: a framework for real-time data processing
- Download full text (pdf) of Nanosurveyor: a framework for real-time data processing
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Near-edge X-ray refraction fine structure microscopy
Part of Applied Physics Letters, 2017
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Part of Scientific Reports, 2016
- DOI for The infectious particle of insect-borne totivirus-like Omono River virus has raised ridges and lacks fibre complexes
- Download full text (pdf) of The infectious particle of insect-borne totivirus-like Omono River virus has raised ridges and lacks fibre complexes
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Open data set of live cyanobacterial cells imaged using an X-ray laser
Part of Scientific Data, 2016
- DOI for Open data set of live cyanobacterial cells imaged using an X-ray laser
- Download full text (pdf) of Open data set of live cyanobacterial cells imaged using an X-ray laser
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Part of Scientific Data, 2016
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A data set from flash X-ray imaging of carboxysomes
Part of Scientific Data, 2016
- DOI for A data set from flash X-ray imaging of carboxysomes
- Download full text (pdf) of A data set from flash X-ray imaging of carboxysomes
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Hummingbird: monitoring and analyzing flash X-ray imaging experiments in real time
Part of Journal of applied crystallography, p. 1042-1047, 2016
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Single-shot diffraction data from the Mimivirus particle using an X-ray free-electron laser
Part of Scientific Data, 2016
- DOI for Single-shot diffraction data from the Mimivirus particle using an X-ray free-electron laser
- Download full text (pdf) of Single-shot diffraction data from the Mimivirus particle using an X-ray free-electron laser
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Data publication with the structural biology data grid supports live analysis
Part of Nature Communications, 2016
- DOI for Data publication with the structural biology data grid supports live analysis
- Download full text (pdf) of Data publication with the structural biology data grid supports live analysis
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CCP-FEL: a collection of computer programs for free-electron laser research
Part of Journal of applied crystallography, p. 1117-1120, 2016
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The trickle before the torrent-diffraction data from X-ray lasers
Part of Scientific Data, 2016
- DOI for The trickle before the torrent-diffraction data from X-ray lasers
- Download full text (pdf) of The trickle before the torrent-diffraction data from X-ray lasers
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SHARP: a distributed GPU-based ptychographic solver
Part of Journal of applied crystallography, p. 1245-1252, 2016
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Three-dimensional reconstruction of the giant mimivirus particle with an X-ray free-electron laser
Part of Physical Review Letters, 2015
- DOI for Three-dimensional reconstruction of the giant mimivirus particle with an X-ray free-electron laser
- Download full text (pdf) of Three-dimensional reconstruction of the giant mimivirus particle with an X-ray free-electron laser
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Imaging single cells in a beam of live cyanobacteria with an X-ray laser
Part of Nature Communications, 2015
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The linac coherent light source single particle imaging road map
Part of Structural Dynamics, 2015
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Part of Optics Express, p. 28914-28925, 2014
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High-throughput imaging of heterogeneous cell organelles with an X-ray laser
Part of Nature Photonics, p. 943-949, 2014
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Chemical composition mapping with nanometre resolution by soft X-ray microscopy
Part of Nature Photonics, p. 765-769, 2014
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Shapes and vorticities of superfluid helium nanodroplets
Part of Science, p. 906-909, 2014
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Part of Journal of applied crystallography, p. 1118-1131, 2014
- DOI for Cheetah: software for high-throughput reduction and analysis of serial femtosecond X-ray diffraction data
- Download full text (pdf) of Cheetah: software for high-throughput reduction and analysis of serial femtosecond X-ray diffraction data
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Part of Optics Express, p. 28729-28742, 2013
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Sensing the wavefront of x-ray free-electron lasers using aerosol spheres
Part of Optics Express, p. 12385-12394, 2013
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The extraction of single-particle diffraction patterns from a multiple-particle diffraction pattern
Part of Optics Express, p. 15102-15112, 2013
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Impact of wave front and coherence optimization in coherent diffractive imaging
Part of Optics Express, p. 11441-11447, 2013
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Part of Journal of Physics B, p. 164033, 2013
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Augmented projections for ptychographic imaging
Part of Inverse Problems, p. 115009, 2013
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Femtosecond free-electron laser x-ray diffraction data sets for algorithm development
Part of Optics Express, p. 4149-4158, 2012
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The Coherent X-ray Imaging Data Bank
Part of Nature Methods, p. 854-855, 2012
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Time-resolved protein nanocrystallography using an X-ray free-electron laser
Part of Optics Express, p. 2706-2716, 2012
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In vivo protein crystallization opens new routes in structural biology
Part of Nature Methods, p. 259-262, 2012
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Lipidic phase membrane protein serial femtosecond crystallography
Part of Nature Methods, p. 263-265, 2012
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Noise-robust coherent diffractive imaging with a single diffraction pattern
Part of Optics Express, p. 16650-16661, 2012
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Femtosecond dark-field imaging with an X-ray free electron laser
Part of Optics Express, p. 13501-13512, 2012
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Part of The international journal of high performance computing applications, p. 378-385, 2012
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Self-terminating diffraction gates femtosecond X-ray nanocrystallography measurements
Part of Nature Photonics, p. 35-40, 2012
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Fractal morphology, imaging and mass spectrometry of single aerosol particles in flight
Part of Nature, p. 513-517, 2012
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Unsupervised classification of single-particle X-ray diffraction snapshots by spectral clustering.
Part of Optics Express, p. 15929-15936, 2011
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Unsupervised classification of single-particle X-ray diffraction snapshots by spectral clustering
Part of Optics Express, p. 16542-16549, 2011
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On the Feasibility of Nanocrystal Imaging Using Intense and Ultrashort X-ray Pulses
Part of ACS Nano, p. 139-146, 2011
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Single mimivirus particles intercepted and imaged with an X-ray laser
Part of Nature, p. 78-81, 2011
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Femtosecond X-ray protein nanocrystallography
Part of Nature, p. 73-77, 2011
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Structure-factor analysis of femtosecond micro-diffraction patterns from protein nanocrystals
Part of Acta Crystallographica Section A, p. 131-140, 2011
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Radiation damage in protein serial femtosecond crystallography using an x-ray free-electron laser
Part of Physical Review B. Condensed Matter and Materials Physics, 2011
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Compressive auto-indexing in femtosecond nanocrystallography
Part of Ultramicroscopy, p. 807-811, 2011
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Part of Physical Review Letters, 2010
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Hawk: the image reconstruction package for coherent X-ray diffractive imaging
Part of Journal of applied crystallography, p. 1535-1539, 2010
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Femtosecond diffractive imaging of biological cells
Part of Journal of Physics B, p. 194015, 2010
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Part of Physical Review E. Statistical, Nonlinear, and Soft Matter Physics, 2009
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Single-Shot Diffractive Imaging with a Table-Top Femtosecond Soft X-Ray Laser-Harmonics Source
Part of Physical Review Letters, 2009
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Single particle X-ray diffractive imaging
Part of Nano letters (Print), p. 310-6, 2008
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Femtosecond diffractive imaging with a soft-X-ray free-electron laser
Part of Nature Physics, p. 839-843, 2006
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Interactive visualization of electron density slices
Part of J. Appl. Cryst., p. 563-565, 2005
Articles, review/survey
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Advancing time-resolved structural biology: latest strategies in cryo-EM and X-ray crystallography
Part of Nature Methods, p. 1420-1435, 2025
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Condor: a simulation tool for flash X-ray imaging
Part of Journal of applied crystallography, p. 1356-1362, 2016
- DOI for Condor: a simulation tool for flash X-ray imaging
- Download full text (pdf) of Condor: a simulation tool for flash X-ray imaging
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Feasibility of imaging living cells at subnanometer resolutions by ultrafast X-ray diffraction
Part of Quarterly reviews of biophysics, p. 181-204, 2008
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Structural studies of melting on the picosecond time scale
Part of Physical Chemistry, Chemical Physics - PCCP, p. 6344-6349, 2008
Comprehensive doctoral thesis
Conference papers
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Ptychographic imaging for the characterization of X-ray free-electron laser beams
Part of X-RAY MICROSCOPY CONFERENCE 2016 (XRM 2016), 2017
- DOI for Ptychographic imaging for the characterization of X-ray free-electron laser beams
- Download full text (pdf) of Ptychographic imaging for the characterization of X-ray free-electron laser beams
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Real-Time Data-Intensive Computing
Part of Proceedings Of The 12Th International Conference On Synchrotron Radiation Instrumentation (SRI2015), 2016
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Profiling structured beams using injected aerosols
Part of Proceedings of SPIE, p. 850403, 2012