Sequencing services at Uppsala Genome Center
We provide both long and short read technologies at UGC, each system with it's specific properties.
After sequencing, we provide at least a basic level of bioinformatic analysis, including runQC, barcode splitting, assembly, and more.
Data is delivered securely to our users with the SciLifeLab DataDeliverySystem (DDS).
Sequencing methods offered at UGC
PacBio Revio
PacBio HiFi sequencing utilizes the Single Molecule Real Time Sequencing (SMRT) technology which provides high sensitivity, high throughput and fast turnaround times. It is suitable for all types of de novo genome sequencing and analysis of structural variation, as well as applications like metabarcode studies and pooled amplicons. Since no amplification is required prior to sequencing, methylations in genomic DNA can be detected by analyzing interpulse duration times.
For more information about PacBio sequencing, visit Pacific Biosciences' website.
We are part of the PacBio Certified Service Provider Program.
Oxford Nanopore PromethION
Oxford Nanopore Technologies (ONT) utilizes a sequencing method where nucleic acid strands (DNA or RNA) pass through nanosized pores embedded in a membrane, over which a current is applied. The resulting base-characteristic changes in current through the pores are detected and translated into base sequences. This provides the opportunity to produce Megabase reads, with methylation information included. The ultra long reads and the high throughput makes nanopore sequencing well suited for, e.g., de novo assembly of complex genomes, structural variation detection, and barcoded or targeted applications.
Since also RNA can be sequenced natively, the method is suitable for RNA modification studies.
For further reading about Oxford Nanopore Technologies, please vistit the Oxford Nanopore website.
