Madlen Hubert
Researcher at Department of Pharmacy; Molecular Pharmaceutics; Cellular Drug Delivery
- E-mail:
- madlen.hubert@uu.se
- Visiting address:
- Biomedicinskt Centrum BMC, Husargatan 3
- Postal address:
- Box 580
751 23 UPPSALA
Short presentation
I am an Associate Professor in Pharmaceutics and my research integrates rational formulation design, biophysics, and molecular biology to understand how therapeutics and target systems interact. I am currently co-leading a work-package within GENEGUT, a Horizon Europe Framework Program consortium, to develop RNA therapy to treat ileal Crohn’s disease and I coordinate a projects on oral delivery of antibiotics funded by the Uppsala Antibiotic Center.
Keywords
- fluorescence microscopy
- biomaterials
- cell culture
- confocal microscopy
- lipid based formulations
- drug delivery
- cell biology
- enabling formulations
- nanoparticles
- self-assembly
- intestinal organoids
- rna therapeutics
Research
I have a background in pharmacy and a PhD in Pharmaceutical Sciences from the University of Otago, New Zealand, with expertise in drug delivery and in vitro models for formulation testing. My research explores lipid-based nanoparticles for oral delivery of challenging therapeutics. I am particularly interested in studying the interactions of nanoparticle with target tissues using a combination of cell-based assays, biophysical approaches as well as advanced microscopy techniques. Additionally, I focus on developing more clinically relevant in vitro models based on human intestinal organoids as a powerful tool in drug development.

Publications
Recent publications
Part of Journal of Colloid and Interface Science, p. 287-300, 2025
- DOI for Liquid crystal nanoparticles for oral combination antibiotic therapies: A strategy towards protecting commensal gut bacteria during treatment
- Download full text (pdf) of Liquid crystal nanoparticles for oral combination antibiotic therapies: A strategy towards protecting commensal gut bacteria during treatment
Antimicrobial Peptides Incorporating Halogenated Marine-Derived Amino Acid Substituents
Part of ACS Medicinal Chemistry Letters, p. 802-809, 2023
Membrane insertion mechanism of the caveola coat protein Cavin1
Part of Proceedings of the National Academy of Sciences of the United States of America, 2022
- DOI for Membrane insertion mechanism of the caveola coat protein Cavin1
- Download full text (pdf) of Membrane insertion mechanism of the caveola coat protein Cavin1
Metallo-β-Lactamase Inhibitor Phosphonamidate Monoesters
Part of ACS Omega, p. 4550-4562, 2022
- DOI for Metallo-β-Lactamase Inhibitor Phosphonamidate Monoesters
- Download full text (pdf) of Metallo-β-Lactamase Inhibitor Phosphonamidate Monoesters
Part of ACS Medicinal Chemistry Letters, p. 257-261, 2022
- DOI for NMR Backbone Assignment of VIM-2 and Identification of the Active Enantiomer of a Potential Inhibitor
- Download full text (pdf) of NMR Backbone Assignment of VIM-2 and Identification of the Active Enantiomer of a Potential Inhibitor
All publications
Articles in journal
Part of Journal of Colloid and Interface Science, p. 287-300, 2025
- DOI for Liquid crystal nanoparticles for oral combination antibiotic therapies: A strategy towards protecting commensal gut bacteria during treatment
- Download full text (pdf) of Liquid crystal nanoparticles for oral combination antibiotic therapies: A strategy towards protecting commensal gut bacteria during treatment
Antimicrobial Peptides Incorporating Halogenated Marine-Derived Amino Acid Substituents
Part of ACS Medicinal Chemistry Letters, p. 802-809, 2023
Membrane insertion mechanism of the caveola coat protein Cavin1
Part of Proceedings of the National Academy of Sciences of the United States of America, 2022
- DOI for Membrane insertion mechanism of the caveola coat protein Cavin1
- Download full text (pdf) of Membrane insertion mechanism of the caveola coat protein Cavin1
Metallo-β-Lactamase Inhibitor Phosphonamidate Monoesters
Part of ACS Omega, p. 4550-4562, 2022
- DOI for Metallo-β-Lactamase Inhibitor Phosphonamidate Monoesters
- Download full text (pdf) of Metallo-β-Lactamase Inhibitor Phosphonamidate Monoesters
Part of ACS Medicinal Chemistry Letters, p. 257-261, 2022
- DOI for NMR Backbone Assignment of VIM-2 and Identification of the Active Enantiomer of a Potential Inhibitor
- Download full text (pdf) of NMR Backbone Assignment of VIM-2 and Identification of the Active Enantiomer of a Potential Inhibitor
PIP5K1 alpha is Required for Promoting Tumor Progression in Castration-Resistant Prostate Cancer
Part of Frontiers in Cell and Developmental Biology, 2022
- DOI for PIP5K1 alpha is Required for Promoting Tumor Progression in Castration-Resistant Prostate Cancer
- Download full text (pdf) of PIP5K1 alpha is Required for Promoting Tumor Progression in Castration-Resistant Prostate Cancer
Part of Journal of Pharmaceutical Sciences, p. 175-184, 2022
- DOI for Comparison of cellular monolayers and an artificial membrane as absorptive membranes in the in vitro lipolysis-permeation assay
- Download full text (pdf) of Comparison of cellular monolayers and an artificial membrane as absorptive membranes in the in vitro lipolysis-permeation assay
Part of Journal of Pharmaceutical Sciences, p. 217-227, 2021
Part of Journal of Pharmaceutical Sciences, p. 176-185, 2021
- DOI for Molecular dynamics simulations reveal membrane interactions for poorly water-soluble drugs: impact of bile solubilization and drug aggregation
- Download full text (pdf) of Molecular dynamics simulations reveal membrane interactions for poorly water-soluble drugs: impact of bile solubilization and drug aggregation
3D-printing of solid lipid tablets from emulsion gels
Part of International Journal of Pharmaceutics, 2021
- DOI for 3D-printing of solid lipid tablets from emulsion gels
- Download full text (pdf) of 3D-printing of solid lipid tablets from emulsion gels
Analysis of Protein and Lipid Interactions Using Liposome Co-sedimentation Assays.
Part of Methods in Molecular Biology, p. 119-127, 2020
Lipid accumulation controls the balance between surface connection and scission of caveolae.
Part of eLIFE, 2020
Part of Journal of Colloid and Interface Science, p. 820-833, 2019
Photoactivated Colibactin Probes Induce Cellular DNA Damage.
Part of Angewandte Chemie International Edition, p. 1417-1421, 2019
Part of European journal of pharmaceutics and biopharmaceutics, p. 10-20, 2018
Part of Journal of Controlled Release, p. 12-22, 2018
Clathrin-Independent Endocytosis Suppresses Cancer Cell Blebbing and Invasion.
Part of Cell Reports, p. 1893-1905, 2017
Part of The Veterinary Record, 2017
Characterization of Lipid-Based Hexosomes as Versatile Vaccine Carriers.
Part of Molecular Pharmaceutics, p. 3945-3954, 2016
Optimisation of one-step desolvation and scale-up of gelatine nanoparticle production.
Part of Journal of Microencapsulation, p. 595-604, 2016
Part of Molecular Pharmaceutics, p. 913-21, 2014
Part of Molecular Pharmaceutics, p. 1928-39, 2013
Part of Crystal Growth & Design, p. 2377-2386, 2009
Articles, review/survey
Part of Biochemical Society Transactions, p. 155-163, 2020
- DOI for Keeping in touch with the membrane: protein- and lipid-mediated confinement of caveolae to the cell surface
- Download full text (pdf) of Keeping in touch with the membrane: protein- and lipid-mediated confinement of caveolae to the cell surface