Molecular Biology Systems

10 credits

Syllabus, Master's level, 1MB466

Code
1MB466
Education cycle
Second cycle
Main field(s) of study and in-depth level
Technology A1N
Grading system
Pass with distinction, Pass with credit, Pass, Fail
Finalised by
The Faculty Board of Science and Technology, 28 February 2023
Responsible department
Biology Education Centre

Entry requirements

120 credits within the Master's Programme in Molecular Biotechnology Engineering, including Cell Biology, Microbiology and Structural Bioinformatics. Proficiency in English equivalent to the Swedish upper secondary course English 6.

Learning outcomes

The course focuses on the central processes of gene expression and regulation in prokaryotic and eukaryotic cells, and biotechnological applications based on these principles.

On completion of the course, students should be able to:

  • describe the common principles of gene expression in prokaryotic and eukaryotic cells.
  • explain mechanisms for and regulation of gene expression, including conceptual differences between prokaryotic and eukaryotic systems.
  • relate changes in the environment to cellular signalling, regulation and physiological response.
  • describe methods that have led to crucial discoveries regarding principles of gene expression, explain current methods of studying gene expression and regulation, and give examples of how these methods are used in biotechnology and drug development.
  • describe the molecular components that selected biotechnical systems consist of and how these cooperate.
  • describe important medical and biotechnological applications that are based on the principles of gene expression and regulation, and discuss societal, ethical and scientific aspects of such applications.
  • apply molecular biology methods of studying gene expression and regulation that are relevant to biotechnical applications, including analysis and interpretation of experimental results.
  • discuss suitability in the choice of methods of solving a biotechnological problem.
  • explain the framework for scientific publication, and read, summarise, discuss and critically review scientific articles in subjects relevant to the course.

Content

  • Concepts and frameworks of gene expression in prokaryotic and eukaryotic cells.
  • Molecular interactions and reaction mechanisms that drive replication of nucleic acids, transcription of DNA, translation of messenger RNA (mRNA), RNA processing, and transport of RNA and proteins.
  • Connection between signals, regulation of gene expression, and physiological adaptations.
  • The cellular context of gene expression, including the cell cycle and the cytoskeleton.
  • Regulatory mechanisms at the transcriptional, post-transcriptional, and post-translational level.
  • Classical and current methods of studying gene expression and regulation, and their use in biotechnology and drug development.
  • Components in and function of biotechnical systems that are relevant to the course.
  • Practical application of molecular biology methods of studying gene expression and regulation that are relevant to biotechnology.
  • Choice of methods of solving biotechnological problems.
  • The principles behind scientific publication and critical review of scientific articles.
  • How knowledge of gene expression and regulation can be applied in medicine and biotechnology, including societal, ethical and scientific aspects of such applications.

Instruction

Lectures, laboratory practicals, and seminars.

Assessment

Written exam (6 credits), laboratory practicals (2 credits), and seminars (2 credits).

If there are special reasons for doing so, an examiner may make an exception from the method of assessment indicated and allow a student to be assessed by another method. An example of special reasons might be a certificate regarding special pedagogical support from the disability coordinator of the university.

No reading list found.

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