Entry requirements

180 credits in biology, bio-/medicine, biotechnology, pharmaceutical biosciences, medical science or equivalent education giving relevant knowledge in cell biology, genetics biochemistry and molecular biology. Proficiency in English equivalent to the Swedish upper secondary course English 6.

Learning outcomes

The course aims to provide an in-depth knowledge in the field of regenerative medicine, from basic biology of stem cells to therapeutic applications.

Knowledge and understanding

On completion of the course, the student shall demonstrate the ability to:

• describe different types of stem cells and their specific characteristics.
• describe methods to culture, differentiate and quality assess human pluripotent stem cells in vitro.
• describe methods, including tissue engineering, to replace damaged or destroyed cells.
• account for regenerative medicine applications to human diseases.
• account for and compare current theories, methods, and techniques within the research field, their practical execution, and application.
• explain the origins of cells used in regenerative medicine, including their developmental and cellular context, such as embryonic, adult, and induced pluripotent stem cells (iPSCs).
• discuss the importance of expanding the scope of regenerative medicine, including interdisciplinary solutions like neuroprosthetics and their integration with cellular therapies for enhanced therapeutic outcomes.
• relate relevant technical developments (e.g. within AI) to regenerative medicine.

Competence and skills

On completion of the course, the student shall demonstrate the ability to:

• execute basic laboratory protocols associated with cell cultures (e.g., seeding, harvesting, expanding, maintaining).
• compile and critically analyse research results and present these both orally and in writing.
• culture mouse neuronal stem cells in the form of neurospheres and adherent cultures.
• culture human pluripotent stem cells in vitro on different matrices.
• evaluate the quality and  maintenance  of cultures of human pluripotent stem cells by morphological inspection and alternative methods.
• execute immunofluorescent staining protocols.

Judgement and approach

On completion of the course, the student shall demonstrate the ability to:

• develop analytical and constructive insights, synthesizing knowledge and findings relating to regenerative medicine. 
• critically evaluate scientific research results (for example be able to discuss how mechanisms from developmental processes are reused in regenerative medicine to restore lost functionality).
• interpret various ethical aspects of regenerative medicine and the implications that restoring lost functions has for individuals. 

Content

The course aims to provide insight into the current knowledge, future potential use and development of regenerative medicine. Different kinds of stem cells will be discussed as well as tissue engineering and their applications in accelerating the healing process to restore injured or damaged tissues and organs. Basic stem cell biology as well as cellular programming and reprogramming will be covered. Clinical applications of regenerative medicine for diseases, such as autoimmune and neuronal diseases, diabetes, and cancer, will be discussed as well as cell and gene therapy.

Laboratory practicals involve stem cell maintenance, differentiation, and specialization analysis using flow cytometry, TaqMan array, and immunofluorescence. Students gain hands-on experience in cell culture, sterile techniques, and essential lab protocols.

Additionally, the course will cover new technologies and models in regenerative medicine, including neuroprosthetics and advanced cell models designed to reduce the use of animals in research. Students will explore how these approaches are improving regenerative therapies and research practices.

The ethical considerations in regenerative medicine will be discussed, and current clinical applications will be illustrated. Development of novel approaches for enhancement, replacement or repair of cells as well as tissues and organs using bioengineered cellular methods and next generation biomaterials will be covered during the course. Criteria in good laboratory/manufacturing practice (GLP/GMP) for applications in regenerative medicine will be discussed as well as policies and commercial development.

Instruction

Instruction is provided in English in the form of lectures and mandatory seminars, laboratory exercises, and study visits.

Assessment

Modes of assessment

The course includes a written exam, a written assignment and an oral presentation in the form of a "journal club," where students analyse and present published scientific literature.

Exceptions to modes of assessment

If there are special reasons for doing so, an examiner may make an exception from the method of assessment indicated and allow a student to be assessed by another method. An example of special reasons might be a certificate regarding special pedagogical support from the University's disability coordinator.

Requirements for a passing grade

To achieve a passing grade, students must pass the written examination, complete the written assignment, and successfully finish all laboratory practicals and related literature assignments. Active participation in all compulsory course components is also required. The final grade is determined by a weighted assessment of performance across all course components.

Other regulations

The course will include the dissection of animal tissues, emphasizing the importance of handling animal materials with care and precision.