Wallenberg Academy Fellowship

Project Summary

Prof. Tenje was selected Wallenberg Academy Fellow in 2016 on a project focused on developing organs-on-chip models of biological barriers, building upon her expertise in microfabrication and microfluidics. During this project, her team has specifically built expertise in two-photon polymerisation (2PP) of hydrogels for 3D cell cultures.

In 2021, Prof. Tenje was granted a Prolongation grant to develop an acoustofluidic toolbox to realise the next generation of in vitro glioblastoma models based on human cerebral organoids. To achieve this, the lab will expand the droplet acoustofluidic technology that she has developed to realise non-contact methods capable of controlling the position of cells in droplets by the use of integrated acoustics and combine this with 2PP fabricated structures.

Collaborators

Prof. Karin Forsberg-Nilsson, Uppsala University

Prof. Mats Nilsson, Stockholm University

Prof. Carl Meinhart, University of California, Santa Barbara

Publications

Peer-reviewed journal articles published in international journals:

15.	A.M. Porras Hernández, M. Tenje and M. Antfolk. Cell chirality exhibition of brain microvascular endothelial cells is dependent on micropattern width. RSC Advances 12 (2022) 30135 – 30144. DOI: 10.1039/D2RA05434E
15.	S-S. Carter, A.R. Atif, A. Diez-Escudero, M. Grape, M-P. Ginebra, M. Tenje and G. Mestres. A microfluidic-based approach to investigate the inflammatory response of macrophages to pristine and drug-loaded nanostructured hydroxyapatite. Materials Today Bio 16 (2022) 100351. DOI: 10.1016/j.mtbio.2022.100351
14.	L. Xiao, S. Johansson, S. Rughöft, F. Burki, M. Mendez Sandin, M. Tenje and L. Behrendt. Photophysiological response of Symbiodiniaceae single cells to temperature stress. The ISME Journal 16 (2022) 2060-2064. DOI: 10.1038/s41396-022-01243-6
13.	L. Xiao, S. Johansson, S. Rughöft, F. Burki, M. Mendez Sandin, M. Tenje and L. Behrendt. Photophysiological response of Symbiodiniaceae single cells to temperature stress. The ISME Journal 16 (2022) 2060-2064. DOI: 10.1038/s41396-022-01243-6
12.	A.R. Atif, U. Lacis, H. Engqvist, M. Tenje, S. Bagheri and G. Mestres. Experimental characterisation and mathematical modelling of the adsorption of proteins and cells on biomimetic hydroxyapatite, ACS Omega 7(1) (2022) 908-920. DOI: 10.1021/acsomega.1c05540
11.	F. Cantoni, G. Werr, L. Barbe, A.M. Porras Hernández and M. Tenje. A microfluidic chip carrier including temperature control and perfusion system for long-term cell imaging. HardwareX 10 (2021) e00245. DOI: 10.1016/j.ohx.2021.e00245
10.	A.M. Porras Hernández, L. Barbe, H. Pohlit, M. Tenje, M. Antfolk. Brain microvascular endothelial cell orientation on micropatterned hydrogels is affected by glucose level variations. Scientific Reports 11 (2021) 19608. DOI: 10.1038/s41598-021-99136-9
9.	A. Fornell, T. Baasch, C. Johannesson, J. Nilsson, M. Tenje. Binary acoustic trapping in a glass capillary, Journal of Physics D: Applied Physics 54 (2021) 355401 (9pp). DOI: 10.1088/1361-6463/ac0841
8.	M. Andersson, S. Johansson, H. Bergman, L. Xiao, L. Behrendt, M. Tenje. A microscopy-compatible temperature regulation system for single-cell phenotype analysis – demonstrated by thermoresponse mapping of microalgea. Lab on a Chip 21 (2021) 1694-1705. DOI: 10.1039/D0LC01288B
7.	A.R. Atif, M. Pujari-Palmer, M. Tenje and G. Mestres. A microfluidic-based method for culturing osteoblasts on biomimetic hydroxyapatite. Acta Biomateriala 127 (2021) 327. DOI: 10.1016/j.actbio.2021.03.046
6.	A. Fornell, H. Pohlit, Q. Shi and M. Tenje. Acoustic focusing of beads and cells in hydrogel droplets. Scientific Reports 11 (2021) 7479. DOI: 10.1038/s41598-021-86985-7
5.	S.S. Carter, L. Barbe, M. Tenje, G. Mestres. Exploring microfluidics as a tool to evaluate the biological properties of a titanium alloy under dynamic conditions, Biomaterials Science 8 (2020) 6309-6321. DOI: 10.1039/d0bm00964d
4.	A.M. Porras Hernández, H. Pohlit, F. Sjögren, L. Shi, D. Ossipov, M. Antfolk and M. Tenje. A simplified approach to control cell adherence on biologically derived in vitro cell culture scaffolds by direct UV-mediated RGD linkage. Journals of Materials Science: Materials in Medicine 31 (2020) 89-98. DOI: 10.1007/s10856-020-06446-x
3.	S.S. Carter, A.R. Atif, S. Kadekar, I. Lanekoff, H. Engqvist, O.P. Varghese, M. Tenje and G. Mestres. PDMS leaching and its implications for on-chip studies focusing on bone-related applications. Organs-on-a-chip 2 (2020) 100004. DOI: 10.1016/j.ooc.2020.100004
2.	A. Fornell, Z. Liu and M. Tenje. Optimisation of the droplet split design for high acoustic particle enrichment in droplet microfluidics. Microelectronic Engineering 226 (2020) 111303 (6 pp). DOI: 10.1016/j.mee.2020.111303
1.	A. Fornell, C. Johannesson. S.S. Searle, A. Happstadius, J. Nilsson and M. Tenje. An acoustofluidic platform for non-contact trapping of cell-laden hydrogel droplets compatible with optical microscopy. Biomicrofluidics 13(4) (2019) 044101. DOI: 10.1063/1.5108583

Review articles:

V. Paloschi, M. Sabater-Lleal, H. Middelkamp, A. Vivas, S. Johansson, A. van der Meer, M. Tenje and L. Maegdefessel. Organ-on-a-chip technology: a novel approach to investigate cardiovascular diseases. Cardiovascular Research 117 (2021) 2742-2754.

DOI: 10.1093/cvr/cvab088

M. Tenje, F. Cantoni, A.M. Porras Hernández, S.S. Searle, S. Johansson, L. Barbe, M. Antfolk and H. Pohlit. A practical guide to microfabrication and patterning of hydrogels for biomimetic cell culture scaffolds. Organs-on-a-chip 2 (2020) 100003.

DOI: 10.1016/j.ooc.2020.100003

Funding

This project has received funding from the Knut and Alice Wallenberg Foundation within their Fellowship programme (KAW 2016.0112 and KAW 2021.0172)

Duration: 01 July 2017 – 30 June 2022 and 01 July 2022 – 30 June 2027

Contact: maria.tenje@angstrom.uu.se