Molecular Tools for Proteome Analysis and Diagnostics, 7.5 credits
Academic year 2022/2023
Spring 2023, 100%, Campus
Start date: 20 March 2023
End date: 26 April 2023
Language of instruction:
Please contact the department.
Entry requirements: 180 credits in biology, bio-/medicine, biotechnology, pharmaceutical biosciences, medical science or equivalent education giving relevant knowledge in cell biology, genetics biochemistry and molecular biology. Proficiency in English equivalent to the Swedish upper secondary course English 6.
About the course
The course will provide an insight into the applications, strategies and implications of molecular analysis methods in research and medicine. Current opinions and research findings, as well as the development of novel methods and technologies, will be conveyed and discussed throughout the course. Different methods for protein analysis, including a variety of affinity-based protein detection methods, will be covered. Molecular tools and technologies such as e.g. affibodies, their use in cancer therapy and LigandTracer, showing protein-cell interactions in real-time, will be discussed.
Molecular diagnostic methods will also be practically illustrated using Proximity ligation assays (PLA) for detection of proteins in solution as well as detection of complex formation in situ. Methods and tools used in the Human Proteome Project, e.g. high-throughout in situ detection of protein expression in different tissues and cancers will be treated. The development of novel molecular diagnostic methods for different diseases, including nanotechnology based diagnostics, and their application in medical clinics will be discussed.