Enzymology and Bioorganic Catalysis
Syllabus, Master's level, 1KB424
- Code
- 1KB424
- Education cycle
- Second cycle
- Main field(s) of study and in-depth level
- Chemistry A1F
- Grading system
- Fail (U), Pass (3), Pass with credit (4), Pass with distinction (5)
- Finalised by
- The Faculty Board of Science and Technology, 11 October 2022
- Responsible department
- Department of Chemistry - BMC
Entry requirements
120 credits. 60 credits in chemistry of which at least 5 at Master's level and including 30 credits in biochemistry. Proficiency in English equivalent to the Swedish upper secondary course English 6.
Learning outcomes
On completion of the course, the student should be able to:
- analyse structure/function relationships in biocatalysed reactions
- predict possible catalytic mechanisms of given reaction types
- present strategies for the analysis of kinetic mechanisms of catalysed reactions
- account for industrial applications of biocatalysis
Content
Reaction kinetics of simple and complex reactions (rate equations, reaction order, molecularity, rate limiting step). Thermodynamic aspects of reactions (reaction coordinates, activated complexes and transition states). Enzyme kinetics (steadystate kinetics, presteadystate kinetics). Reaction mechanisms (ligand binding; catalytic groups: acid/base, nucleophiles, electrophiles, cofactors, metals and entropic effects). Experimental analysis of catalytic and kinetic mechanisms (spectrometry, Xray crystallography, stopped flow, isotope effects, structure/reactivity relationships). Mathematical data analysis (regression analysis, model discrimination). Modelling of enzymatic reactions. Stereo chemistry (mechanisms). Structure/function relationships (protein folding, mutagenesis, enzyme inhibition, evolution of catalytic mechanism, catalytic antibodies). Catalytic nucleic acids (ribozymes, catalytic DNA).
Instruction
The course includes lectures, groups discussions, tutorials and laboratory practicals. Participation in discussions, tutorials and laboratory practicals is mandatory. In addition, a mandatory literature project should be carried out.
Assessment
Written exam (8 credits). Mandatory course components, tutorials, laboratory practicals and literature project which is presented orally and in writing corresponds to 7 points. The final grade is weighted from the results on the written exam and the practical part.
If there are special reasons for doing so, an examiner may make an exception from the method of assessment indicated and allow a student to be assessed by another method. An example of special reasons might be a certificate regarding special pedagogical support from the disability coordinator of the university.
Other directives
The course can not be included in higher education qualification together with 1KB463 Enzymology and Bioorganic Catalysis, 15 cr.