Syllabus for Bioimaging and Cell Analysis

Bioimaging och cellanalys


  • 7.5 credits
  • Course code: 3MG036
  • Education cycle: Second cycle
  • Main field(s) of study and in-depth level: Molecular Medicine A1N, Medical Science A1N

    Explanation of codes

    The code indicates the education cycle and in-depth level of the course in relation to other courses within the same main field of study according to the requirements for general degrees:

    First cycle

    • G1N: has only upper-secondary level entry requirements
    • G1F: has less than 60 credits in first-cycle course/s as entry requirements
    • G1E: contains specially designed degree project for Higher Education Diploma
    • G2F: has at least 60 credits in first-cycle course/s as entry requirements
    • G2E: has at least 60 credits in first-cycle course/s as entry requirements, contains degree project for Bachelor of Arts/Bachelor of Science
    • GXX: in-depth level of the course cannot be classified

    Second cycle

    • A1N: has only first-cycle course/s as entry requirements
    • A1F: has second-cycle course/s as entry requirements
    • A1E: contains degree project for Master of Arts/Master of Science (60 credits)
    • A2E: contains degree project for Master of Arts/Master of Science (120 credits)
    • AXX: in-depth level of the course cannot be classified

  • Grading system: Fail (U), Pass (G), Pass with distinction (VG)
  • Established: 2011-08-26
  • Established by: The Educational Board of Medicine/Chair
  • Revised: 2018-09-21
  • Revised by: The Master Programmes Board of the Faculty of Medicine
  • Applies from: Autumn 2018
  • Entry requirements:

    180 credits in biology, bio-/medicine, biotechnology, pharmaceutical biosciences, medical science or equivalent education providing relevant knowledge in cell biology, genetics, biochemistry and molecular biology. Proficiency in English equivalent to the Swedish upper secondary course English 6.

  • Responsible department: Department of Immunology, Genetics and Pathology

Learning outcomes

The course aims to provide an in-depth knowledge of bioimaging settings from single molecules to man used in medical and research applications.

After completing the course, the student should be able to

  • describe different types of imaging instrumentation and their applications
  • account for different types of image analysis techniques and their applications
  • understand the concept of fluorescence and how it is applied in the context of microscopy
  • account for and evaluate current theories, methods and techniques within the research field, their practical execution and application
  • compile, critically analyse and evaluate research results and present these both orally and in writing.


The course aims to provide an insight into the current knowledge as well as the future use and development of bioimaging methods as analytical and diagnostic tools in research and health care.

Theoretical principles for different types of microscopy detection methods, e.g. fluorescence, confocal and multiphoton will be discussed and demonstrated. Electron microscopy will be discussed. Detection of single-molecules in situ using FISH, FRET (Fluorescence Resonance Energy Transfer) as well as FRAP methods will be discussed.

Cytochemistry and preparation of samples will be covered. Cellular analysis methods will be illustrated by analytical and preparative flow cytometry. Digital image analysis will be illustrated both theoretically and in practical exercises. Quantitative imaging as well as in vivo imaging or live cell imaging will be discussed.

Medical imaging analysis methods used in clinical diagnostics such as whole body analysis and Positron emission tomography (PET), a nuclear medicine imaging technique will be illustrated. Current methods as well as development of novel methods and technologies for clinical and research applications, will be discussed.


The teaching is given in english in form of lectures and compulsory seminars, demonstrations, literature assignments, presentations related to current research fields as well as theoretical and practical computational laboratory sessions.


Modules: Theory and seminars 4 credits; Laboratory session 3.5 credits

The module laboratory sessions require completed laboratory practicals and related literature assignments. The theoretical module and seminars require active participation. An examination is given at the end of the course. To pass the requirements of the curriculum, all assigned tasks must be completed.

If there are special reasons for doing so, an examiner may make an exception from the method of assessment indicated and allow a student to be assessed by another method. An example of special reasons might be a certificate regarding special pedagogical support from the University's disability coordinator.

Other directives

Selection to course: 1. Students submitted to the Master programme in Molecular Medicine. 2. Other students.

Reading list

Reading list

Applies from: Autumn 2021

Some titles may be available electronically through the University library.

Course Literature:

Scientific articles