Research expertise and methodologies

• Antibiotic interactions detection: CombiANT, SIF, checkerboards, time-kil.
• Growth/Fitness
• Gene expression level
• Promoter mapping
• Transcriptional/translational fusions
• Applications with thermophilic bacteria

Ongoing research projects

Infections rarely happen in isolated, but complex contexts, frequently with more than one drug prescribed and/or with other microorganisms present. I am interested in understanding how antibiotics interact between them and the complex interactions than happen between different bacterial species, especially in the context of antimicrobial treatments.

Before joining Uppsala research community, I used to work on physiology on environmental bacteria, both mesophilic and thermophilic. Now, I used that regulatory background to study antibiotic interactions.

Key Publications

1. Sánchez-Hevia D.L., Fatsis-Kavalopoulos N., Andersson D.I. (2025) Sublethal interaction factor (SIF), a growth-based method to analyze antibiotic combinations at sub-inhibitory concentrations. Microbiology Spectrum, 13(12):e0107025. https://doi.org/10.1128/spectrum.01070-25
2. Tang P-C., Sánchez-Hevia D.L., Westhoff S., Fatsis-Kavalopoulos N., Andersson D.I. (2024) Within-species variability of antibiotic interactions in Gram-negative bacteria. mBio, 15(3):e0019624. https://doi.org/10.1128/mbio.00196-24
3. Sánchez-Hevia D.L., Yuste L., Moreno R., Rojo F. (2018) Influence of the Hfq and Crc global regulators on the control of iron homeostasis in Pseudomonas putida. Environmental Microbiology, 20(10):3484–3503. https://doi.org/10.1111/1462-2920.14263