Entry requirements

120 credits with 60 credits in chemistry including Separation and Mass Spectrometry, 15 credits. Proficiency in English equivalent to the Swedish upper secondary course English 6.

Learning outcomes

On completion of the course, the student should be able to:

• compare different ionization source types based on their theoretical and practical differences, and account for commonly observed ion phenomena,
• compare and contrast instrumental developments in mass spectrometry and ion mobility mass spectrometry, and assess theirpractical  utility within challenging areas of analytical chemistry,
• account for sample preparation, analysis and data evaluation approaches to protein analysis in modern mass spectrometry based proteomics,
• justify approaches to targeted and non-targeted analysis in modern mass spectrometry based metabolomics and spatial mass spectrometry,
• perform experimental work with MS instrumentation and apply modern data evaluation tools to complex datasets to extract the rich information available from optimized analytical approaches,
• critically analyze MS related problems and their solutions,and prepare a written account of literature results in the form of a scientific article.

Content

Modern approaches to ionization of samples and the practical limitations of molecular coverage in various ionization techniques. Observed ion phenomena such as space charge effects and ion fragmentation. Description and justification of the design of advanced high-resolution instrumentation (TOF, FT-ICR-MS, Orbitrap). Demonstration and discussion of cutting edge application areas including metabolomics, proteomics and spatial mass spectrometry. Conditions and opportunities offered for different combinations of separation and imaging techniques coupled to mass spectrometry, with both qualitative and quantitative analytical aspects. Demonstration and discussion of computational tools related to data analysis. Advanced aspects of mass spectrometry in a chosen research topic are explored in a literature review.

Instruction

The teaching is given as lectures, excecises, group discussion, experimental work, demonstrations, seminars and communication training (oral and written).

Assessment

Written examination (7,5 hp). The laboratory experiments and presentations (oral and written) represents 7,5 hp. 

If there are special reasons for doing so, an examiner may make an exception from the method of assessment indicated and allow a student to be assessed by another method. An example of special reasons might be a certificate regarding special pedagogical support from the disability coordinator of the university.

Other directives

Laboratory work and communication training are mandatory. This course cannot be included in a degree together with the course 1KB158 Advanced Mass Spectrometry, 10.0 credits