Land group

Henrik Land introbild

Discovery and protein engineering of redox active metalloenzymes.

Our research

The Land group focuses on discovery and protein engineering of redox active metalloenzymes. Our research is interdisciplinary and combines protein engineering, bioinorganic chemistry and biophysics on enzymes that catalyze environmentally relevant reactions.

Our lab houses an uninterrupted anaerobic platform for screening of enzyme libraries that enables directed evolution of oxygen sensitive enzymes. This includes the ability to perform anaerobic protein expression, cell harvest and spectrophotometric analysis.

More information about our research.

Group members

Research leader: Henrik Land

Group members: Maximilian Böhm

Publications

Probing the Influence of the Protein Scaffold on H-Cluster Reactivity via Gain-of-Function Studies─Improved H₂ Evolution and O₂ Tolerance through Rational Design of [FeFe] Hydrogenase

Cabotaje, Princess R.; Sekretareva, Alina; Senger, Moritz; Huang, Ping et al.

Part of Journal of the American Chemical Society, p. 4654-4666, 2025
- DOI for Probing the Influence of the Protein Scaffold on H-Cluster Reactivity via Gain-of-Function Studies─Improved H₂ Evolution and O₂ Tolerance through Rational Design of [FeFe] Hydrogenase
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Structure-guided engineering of α-ketoisocaproate dioxygenase increases isobutene production in Synechocystis sp. PCC 6803

Schumann, Conrad; Kugler, Amit; Shah, Bhavik Ashwin; Berggren, Gustav et al.

Part of Microbial Cell Factories, 2025
- DOI for Structure-guided engineering of α-ketoisocaproate dioxygenase increases isobutene production in Synechocystis sp. PCC 6803
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Indications for a universal hydrogen catalysis mechanism in [FeFe]-hydrogenases of different phylogenetic groups

Senger, Moritz; Schumann, Conrad; Cabotaje, Princess R.; Zamader, Afridi et al.

Part of Physical Chemistry, Chemical Physics - PCCP, p. 9864-9875, 2025
- DOI for Indications for a universal hydrogen catalysis mechanism in [FeFe]-hydrogenases of different phylogenetic groups
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Minimal and hybrid hydrogenases are active from archaea

Greening, Chris; Cabotaje, Princess R.; Valentin Alvarado, Luis E.; Leung, Pok Man et al.

Part of Cell, 2024
- DOI for Minimal and hybrid hydrogenases are active from archaea
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Photobiocatalytic CO₂ reduction into CO by organic nanorod-carbon monoxide dehydrogenase assemblies: surfactant matters

Pavliuk, Mariia V.; Böhm, Maximilian; Wilhelmsen, Janna; Hardt, Steffen et al.

Part of Chemical Science, p. 16789-16795, 2024
- DOI for Photobiocatalytic CO₂ reduction into CO by organic nanorod-carbon monoxide dehydrogenase assemblies: surfactant matters
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Secondary structure changes as the potential H₂ sensing mechanism of group D [FeFe]-hydrogenases

Voloshyn, Ivan; Schumann, Conrad; Cabotaje, Princess R.; Zamader, Afridi et al.

Part of Chemical Communications, p. 10914-10917, 2024
- DOI for Secondary structure changes as the potential H₂ sensing mechanism of group D [FeFe]-hydrogenases
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Probing Substrate Transport Effects on Enzymatic Hydrogen Catalysis: An Alternative Proton Transfer Pathway in Putatively Sensory [FeFe] Hydrogenase

Cabotaje, Princess R.; Walter, Kaija; Zamader, Afridi; Huang, Ping et al.

Part of ACS Catalysis, p. 10435-10446, 2023
- DOI for Probing Substrate Transport Effects on Enzymatic Hydrogen Catalysis: An Alternative Proton Transfer Pathway in Putatively Sensory [FeFe] Hydrogenase
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Light-Driven [FeFe] Hydrogenase Based H-2 Production in E. coli: A Model Reaction for Exploring E. coli Based Semiartificial Photosynthetic Systems

Lorenzi, Marco; Gamache, Mira T.; Redman, Holly J.; Land, Henrik et al.

Part of ACS Sustainable Chemistry and Engineering, p. 10760-10767, 2022
- DOI for Light-Driven [FeFe] Hydrogenase Based H-2 Production in E. coli: A Model Reaction for Exploring E. coli Based Semiartificial Photosynthetic Systems
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Enantioselective Synthesis of Pharmaceutically Relevant Bulky Arylbutylamines Using Engineered Transaminases

Sheludko, Yuriy, V; Slagman, Sjoerd; Gittings, Samantha; Charnock, Simon J. et al.

Part of Advanced Synthesis and Catalysis, p. 2972-2981, 2022
- DOI for Enantioselective Synthesis of Pharmaceutically Relevant Bulky Arylbutylamines Using Engineered Transaminases
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Reversible or Irreversible Catalysis of H⁺/H₂ Conversion by FeFe Hydrogenases

Fasano, Andrea; Land, Henrik; Fourmond, Vincent; Berggren, Gustav et al.

Part of Journal of the American Chemical Society, p. 20320-20325, 2021
- DOI for Reversible or Irreversible Catalysis of H⁺/H₂ Conversion by FeFe Hydrogenases
Semi-synthetic hydrogenases—in vitro and in vivo applications

Meszaros, Livia S.; Land, Henrik; Redman, Holly J.; Berggren, Gustav

Part of Current Opinion in Green and Sustainable Chemistry, 2021
- DOI for Semi-synthetic hydrogenases—in vitro and in vivo applications
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Semisynthetic [FeFe]-hydrogenase with stable expression and H₂ production capacity in a photosynthetic microbe

Wegelius, Adam; Land, Henrik; Berggren, Gustav; Lindblad, Peter

Part of Cell Reports Physical Science, 2021
- DOI for Semisynthetic [FeFe]-hydrogenase with stable expression and H₂ production capacity in a photosynthetic microbe
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Carbon Dots and [FeFe] Hydrogenase Biohybrid Assemblies for Efficient Light-Driven Hydrogen Evolution

Hola, Katerina; Pavliuk, Mariia V.; Nemeth, Brigitta; Huang, Ping et al.

Part of ACS Catalysis, p. 9943-9952, 2020
- DOI for Carbon Dots and [FeFe] Hydrogenase Biohybrid Assemblies for Efficient Light-Driven Hydrogen Evolution
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Engineering the Active Site of an (S)-Selective Amine Transaminase for Acceptance of Doubly Bulky Primary Amines

Land, Henrik; Ruggieri, Federica; Szekrenyi, Anna; Fessner, Wolf-Dieter et al.

Part of Advanced Synthesis and Catalysis, p. 812-821, 2020
- DOI for Engineering the Active Site of an (S)-Selective Amine Transaminase for Acceptance of Doubly Bulky Primary Amines
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Characterization of a putative sensory [FeFe]-hydrogenase provides new insight into the role of the active site architecture

Land, Henrik; Sekretaryova, Alina N.; Huang, Ping; Redman, Holly J. et al.

Part of Chemical Science, p. 12789-12801, 2020
- DOI for Characterization of a putative sensory [FeFe]-hydrogenase provides new insight into the role of the active site architecture
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Current State of [FeFe]-Hydrogenase Research: Biodiversity and Spectroscopic Investigations

Land, Henrik; Senger, Moritz; Berggren, Gustav; Stripp, Sven T.

Part of ACS Catalysis, p. 7069-7086, 2020
- DOI for Current State of [FeFe]-Hydrogenase Research: Biodiversity and Spectroscopic Investigations
The maturase HydF enables [FeFe] hydrogenase assembly via transient, cofactor-dependent interactions

Németh, Brigitta; Land, Henrik; Magnuson, Ann; Hofer, Anders et al.

Part of Journal of Biological Chemistry, p. 11891-11901, 2020
- DOI for The maturase HydF enables [FeFe] hydrogenase assembly via transient, cofactor-dependent interactions
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B-factor Guided Proline Substitutions in Chromobacterium violaceum Amine Transaminase: Evaluation of the Proline Rule as a Method for Enzyme Stabilization

Land, Henrik; Campillo-Brocal, Jonatan C.; Humble, Maria Svedendahl; Berglund, Per

Part of ChemBioChem, p. 1297-1304, 2019
- DOI for B-factor Guided Proline Substitutions in Chromobacterium violaceum Amine Transaminase: Evaluation of the Proline Rule as a Method for Enzyme Stabilization
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Discovery of novel [FeFe]-hydrogenases for biocatalytic H-2-production

Land, Henrik; Ceccaldi, Pierre; Meszaros, Livia S.; Lorenzi, Marco et al.

Part of Chemical Science, p. 9941-9948, 2019
- DOI for Discovery of novel [FeFe]-hydrogenases for biocatalytic H-2-production
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Contact

Henrik Land