Joakim Näsvall
Forskare vid Institutionen för medicinsk biokemi och mikrobiologi; Infektioner och Immunitet; Joakim Näsvall
- Mobiltelefon:
- 070-697 22 36
- E-post:
- joakim.nasvall@imbim.uu.se
- Besöksadress:
- BMC
Husargatan 3
752 37 UPPSALA - Postadress:
- Box 582
751 23 UPPSALA
- ORCID:
- 0000-0002-6831-3105
Forskning
Evolution av nya gener
Vår forskning fokuserar på en väldigt grundläggande fråga i evolutionsbiologi; hur nya gener bildas och hur de evolverar. En ny gen kan uppkomma i ett genom på tre olika sätt:
(A) Horisontell (lateral) genöverföring, då en gen förs over från en annan organism. (B) De novo-evolution, då en funktion uppkommer i en tidigare icke-funktionell DNA-sekvens. (C) Duplikation-divergens, då en ny funktion evolverar i en kopia av en duplicerad gen. Vår nuvarande forskning berör i huvudsak duplikation-divergens.
Vi använder experimentell evolution och genetiska metoder i bakteriella modellsystem för att studera hur genduplikation och –amplifiering, neutrala mutationer, “trade-offs” mellan olika funktioner och andra faktorer påverkar uppkomsten av nya funktioner och deras vidare evolution. Vi är också intresserade av andra frågor, som evolution av komponenterna i translationsapparaten och den genetiska koden.
Publikationer
Urval av publikationer
Collateral toxicity limits the evolution of bacterial Release Factor 2 towards total omnipotence
Ingår i Molecular biology and evolution, s. 2918-2930, 2020
- DOI för Collateral toxicity limits the evolution of bacterial Release Factor 2 towards total omnipotence
- Ladda ner fulltext (pdf) av Collateral toxicity limits the evolution of bacterial Release Factor 2 towards total omnipotence
Ingår i Frontiers in Microbiology, 2020
- DOI för Synonymous mutations in rpsT lead to ribosomal assembly defects that can be compensated by mutations in fis and rpoA
- Ladda ner fulltext (pdf) av Synonymous mutations in rpsT lead to ribosomal assembly defects that can be compensated by mutations in fis and rpoA
Genetic adaptation to growth under laboratory conditions in Escherichia coli and Salmonella enterica
Ingår i Frontiers in Microbiology, 2018
- DOI för Genetic adaptation to growth under laboratory conditions in Escherichia coli and Salmonella enterica
- Ladda ner fulltext (pdf) av Genetic adaptation to growth under laboratory conditions in Escherichia coli and Salmonella enterica
Ingår i Molecular biology and evolution, s. 704-718, 2018
- DOI för Experimental Determination and Prediction of the Fitness Effects of Random Point Mutations in the Biosynthetic Enzyme HisA
- Ladda ner fulltext (pdf) av Experimental Determination and Prediction of the Fitness Effects of Random Point Mutations in the Biosynthetic Enzyme HisA
Ingår i PLOS ONE, 2017
- DOI för Direct and Inverted Repeat stimulated excision (DIRex): Simple, single-step, and scar-free mutagenesis of bacterial genes
- Ladda ner fulltext (pdf) av Direct and Inverted Repeat stimulated excision (DIRex): Simple, single-step, and scar-free mutagenesis of bacterial genes
Ingår i Nucleic Acids Research, 2017
- DOI för Duplication-Insertion Recombineering: a fast and scar-free method for efficient transfer of multiple mutations in bacteria
- Ladda ner fulltext (pdf) av Duplication-Insertion Recombineering: a fast and scar-free method for efficient transfer of multiple mutations in bacteria
Evolution of New Functions De Novo and from Preexisting Genes
Ingår i Cold Spring Harbor Perspectives in Biology, 2015
Real-Time Evolution of New Genes by Innovation, Amplification, and Divergence
Ingår i Science, s. 384-387, 2012
Activation of cryptic aminoglycoside resistance in Salmonella enterica
Ingår i Molecular Microbiology, s. 1464-1478, 2011
Senaste publikationer
Collateral toxicity limits the evolution of bacterial Release Factor 2 towards total omnipotence
Ingår i Molecular biology and evolution, s. 2918-2930, 2020
- DOI för Collateral toxicity limits the evolution of bacterial Release Factor 2 towards total omnipotence
- Ladda ner fulltext (pdf) av Collateral toxicity limits the evolution of bacterial Release Factor 2 towards total omnipotence
Ingår i Frontiers in Microbiology, 2020
- DOI för Synonymous mutations in rpsT lead to ribosomal assembly defects that can be compensated by mutations in fis and rpoA
- Ladda ner fulltext (pdf) av Synonymous mutations in rpsT lead to ribosomal assembly defects that can be compensated by mutations in fis and rpoA
Ingår i Frontiers in Microbiology, 2020
- DOI för Mutational Pathways and Trade-Offs Between HisA and TrpF Functions: Implications for Evolution via Gene Duplication and Divergence
- Ladda ner fulltext (pdf) av Mutational Pathways and Trade-Offs Between HisA and TrpF Functions: Implications for Evolution via Gene Duplication and Divergence
Selection for novel metabolic capabilities in Salmonella enterica
Ingår i Evolution, s. 990-1000, 2019
- DOI för Selection for novel metabolic capabilities in Salmonella enterica
- Ladda ner fulltext (pdf) av Selection for novel metabolic capabilities in Salmonella enterica
Ingår i Journal of Biological Chemistry, s. 11481-11490, 2018
- DOI för Structural mechanism of AadA, a dual specificity aminoglycoside adenylyltransferase from Salmonella enterica
- Ladda ner fulltext (pdf) av Structural mechanism of AadA, a dual specificity aminoglycoside adenylyltransferase from Salmonella enterica
Alla publikationer
Artiklar i tidskrift
Collateral toxicity limits the evolution of bacterial Release Factor 2 towards total omnipotence
Ingår i Molecular biology and evolution, s. 2918-2930, 2020
- DOI för Collateral toxicity limits the evolution of bacterial Release Factor 2 towards total omnipotence
- Ladda ner fulltext (pdf) av Collateral toxicity limits the evolution of bacterial Release Factor 2 towards total omnipotence
Ingår i Frontiers in Microbiology, 2020
- DOI för Synonymous mutations in rpsT lead to ribosomal assembly defects that can be compensated by mutations in fis and rpoA
- Ladda ner fulltext (pdf) av Synonymous mutations in rpsT lead to ribosomal assembly defects that can be compensated by mutations in fis and rpoA
Ingår i Frontiers in Microbiology, 2020
- DOI för Mutational Pathways and Trade-Offs Between HisA and TrpF Functions: Implications for Evolution via Gene Duplication and Divergence
- Ladda ner fulltext (pdf) av Mutational Pathways and Trade-Offs Between HisA and TrpF Functions: Implications for Evolution via Gene Duplication and Divergence
Selection for novel metabolic capabilities in Salmonella enterica
Ingår i Evolution, s. 990-1000, 2019
- DOI för Selection for novel metabolic capabilities in Salmonella enterica
- Ladda ner fulltext (pdf) av Selection for novel metabolic capabilities in Salmonella enterica
Ingår i Journal of Biological Chemistry, s. 11481-11490, 2018
- DOI för Structural mechanism of AadA, a dual specificity aminoglycoside adenylyltransferase from Salmonella enterica
- Ladda ner fulltext (pdf) av Structural mechanism of AadA, a dual specificity aminoglycoside adenylyltransferase from Salmonella enterica
Genetic adaptation to growth under laboratory conditions in Escherichia coli and Salmonella enterica
Ingår i Frontiers in Microbiology, 2018
- DOI för Genetic adaptation to growth under laboratory conditions in Escherichia coli and Salmonella enterica
- Ladda ner fulltext (pdf) av Genetic adaptation to growth under laboratory conditions in Escherichia coli and Salmonella enterica
Ingår i Molecular biology and evolution, s. 704-718, 2018
- DOI för Experimental Determination and Prediction of the Fitness Effects of Random Point Mutations in the Biosynthetic Enzyme HisA
- Ladda ner fulltext (pdf) av Experimental Determination and Prediction of the Fitness Effects of Random Point Mutations in the Biosynthetic Enzyme HisA
Ingår i PLOS ONE, 2017
- DOI för Direct and Inverted Repeat stimulated excision (DIRex): Simple, single-step, and scar-free mutagenesis of bacterial genes
- Ladda ner fulltext (pdf) av Direct and Inverted Repeat stimulated excision (DIRex): Simple, single-step, and scar-free mutagenesis of bacterial genes
Ingår i Nucleic Acids Research, 2017
- DOI för Duplication-Insertion Recombineering: a fast and scar-free method for efficient transfer of multiple mutations in bacteria
- Ladda ner fulltext (pdf) av Duplication-Insertion Recombineering: a fast and scar-free method for efficient transfer of multiple mutations in bacteria
Structural and functional innovations in the real-time evolution of new (βα)8 barrel enzymes
Ingår i Proceedings of the National Academy of Sciences of the United States of America, s. 4727-4732, 2017
- DOI för Structural and functional innovations in the real-time evolution of new (βα)8 barrel enzymes
Evolution of antibiotic resistance without antibiotic exposure
Ingår i Antimicrobial Agents and Chemotherapy, 2017
- DOI för Evolution of antibiotic resistance without antibiotic exposure
- Ladda ner fulltext (pdf) av Evolution of antibiotic resistance without antibiotic exposure
Compensating the fitness costs of synonymous mutations
Ingår i Molecular biology and evolution, s. 1461-1477, 2016
- DOI för Compensating the fitness costs of synonymous mutations
- Ladda ner fulltext (pdf) av Compensating the fitness costs of synonymous mutations
Ingår i Journal of Biological Chemistry, s. 24657-24668, 2015
Structure of AadA from Salmonella enterica: a monomeric aminoglycoside (3'')(9) adenyltransferase
Ingår i Acta Crystallographica Section D, s. 2267-2277, 2015
- DOI för Structure of AadA from Salmonella enterica: a monomeric aminoglycoside (3'')(9) adenyltransferase
- Ladda ner fulltext (pdf) av Structure of AadA from Salmonella enterica: a monomeric aminoglycoside (3'')(9) adenyltransferase
Minor Fitness Costs in an Experimental Model of Horizontal Gene Transfer in Bacteria
Ingår i Molecular biology and evolution, s. 1220-1227, 2014
Real-Time Evolution of New Genes by Innovation, Amplification, and Divergence
Ingår i Science, s. 384-387, 2012
Activation of cryptic aminoglycoside resistance in Salmonella enterica
Ingår i Molecular Microbiology, s. 1464-1478, 2011
Artiklar, forskningsöversikt
Evolution of New Functions De Novo and from Preexisting Genes
Ingår i Cold Spring Harbor Perspectives in Biology, 2015
Manuskript (preprint)
Synonymous mutations that reduce S20 levels can be compensated by mutations in fis and rpoA
Functional trade-offs during evolution of new functions in the HisA-TrpF system
Experimental evolution of novel metabolic capabilities in Salmonella enterica
Ribosomal protein L1 is required for balanced formation of ribosomal subunits
Crystal structure of AadA at 2.5 Å resolution - an aminoglycoside 3" adenyltransferase
Intermittent selection directs evolution of a specialist enzyme to become a generalist