Ola Söderberg

Professor vid Institutionen för farmaceutisk biovetenskap; Forskning; Farmaceutisk cellbiologi

Telefon:: 018-471 45 29
Mobiltelefon:: 072-999 93 34
E-post:: ola.soderberg@uu.se
Besöksadress:: BMC, Husargtan 3
75124 uppsala
Postadress:: Box 591
75124 Uppsala

Ladda ned kontaktuppgifter för Ola Söderberg vid Institutionen för farmaceutisk biovetenskap; Forskning; Farmaceutisk cellbiologi

Akademiska meriter:: docent
CV:: Ladda ned CV
ORCID:: 0000-0003-2883-1925

Forskning

My on-going research and future plans include both method development to provide better tools for investigating cell biology and application of these new methods in basic research and for improved diagnostics of e.g. cancer and infectious diseases. Since the development of the in situ proximity ligation assay (in situ PLA) (Söderberg et al., Nat Methods, 2006), most of our efforts have been related to the use of in situ PLA and to further improve the method, e.g. for multiplex analysis, simultaneous measurement of proteins and mRNA and for detection of protein-DNA interactions. In situ PLA combines multiple recognition of affinity reagents with potent signal amplification, utilizing methods for DNA analysis to generate a signal that will be a surrogate marker of the targeted protein, protein-protein interaction or post-translational modifications of proteins. The method is based on pairs of proximity-probes (i.e. antibodies conjugated to strands of DNA) to detect the proteins of interest. Only upon proximal binding of these probes can an amplifiable DNA molecule be generated by ligation, which enhance the selectivity of the method even further. We have also been working on developing novel methods, such as proximity-dependent initiation of hybridization chain reaction (proxHCR) (Koos et al., Nat Commun, 2015). ProxHCR retains the dual binding requirement of in situ PLA while not needing any enzymatic steps. Our ongoing work is focused on developing methods that will provide a deeper understanding on the biochemistry of the cell, and for analyses of DNA and RNA. Our recent development of the STEEL-seq method, provides a new tool to study single-strand DNA breaks (Wenson et al. Nat Commun, 2025).

Publikationer

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Artiklar i tidskrift

Artiklar, forskningsöversikt

Kapitel i böcker, delar av antologi

Protein Diagnostics by Proximity Ligation: Combining Multiple Recognition and DNA Amplification for Improved Protein Analyses

Cane, Gaëlle; Leuchowius, Karl-Johan; Söderberg, Ola; Kamali-Moghaddam, Masood et al.

Ingår i Molecular Diagnostics (Third Edition), s. 219-231, Academia Press, 2017
Analysis of protein interactions in situ by proximity ligation assays

Koos, Björn; Andersson, Linda; Clausson, Carl-Magnus; Grannas, Karin et al.

Ingår i High-Dimensional Single Cell Analysis, s. 111-26, Springer, 2014
- DOI för Analysis of protein interactions in situ by proximity ligation assays
Protein diagnostics by proximity ligation: Combining multiple recognition and DNA amplification for improved protein analysis

Leuchowius, Karl-Johan; Söderberg, Ola; Kamali-Moghaddam, Masood; Jarvius, Malin et al.

Ingår i Molecular Diagnostics, s. 299-306, Elsevier/Academic Press, 2010
Proximity ligation: a specific and versatile tool for the proteomic era.

Söderberg, Ola; Leuchowius, Karl-Johan; Kamali-Moghaddam, Masood; Jarvius, Malin et al.

Ingår i Genet Eng (N Y)., s. 190, Springer, 2007