Cryo-EM Uppsala

Sample preparation

A Mark IV Vitrobot (Thermo Fisher) is available for sample vitrification. Samples may be screened and analyzed in-house or shipped/transported to another facility for data collection.

Sample screening and data collection

A 200kV Glacios electron microscope mounted with a Falcon 4i direct electron detector and Selectris energy filter is available for efficient sample screening and/or data collection. The Glacios is equipped with an autoloader that allows loading of up to 12 grids for continuous analysis for up to 3 days. The Falcon 4i direct electron detector is capable of collecting 200-1000 images/hours at high-resolution depending on conditions.

Data analysis and image processing

Cryo-EM Uppsala houses a local GPU cluster for on-the-fly image processing and sample optimization, and 3D processing and model building using a wide variety of software packages, including Relion and Cryosparc.

Starting a new project

Cryo-EM Uppsala offers tailor-made solutions based on your goals and previous cryo-EM experience. To start a new project, follow the instructions on the Cryo-EM Uppsala iLab Booking System.

TRAINING

The goal of Cryo-EM Uppsala is to service both new and experienced cryo-EM users at a conveniently located facility at the BMC. Experienced users may operate the instrumentation independently after training. New users will be offered training to gain the knowledge and hands-on experience needed to operate the instrumentation. We are also regulary giving workshops and a 1-month course (3 hp) for PhD students.

Please contact the facility for further information about training opportunities at cryoem-icm@uu.se.

RATES

Booking of the instruments are done using the Cryo-EM Uppsala iLab Booking System. Cancellation should be done at least 24 h before the start of the session.

VitroBot

• 600 SEK/session (consumables are available at cost through iLab)

Glacios

• Day: 2,500 SEK (6 hrs, 9.00-15.00)
• ​Night: 2,500 SEK (18 hrs, 15.00-09.00 the day after)
• Weekend Data Collection: 7,500 SEK (66 hrs, 15,00 Friday to 09.00 Monday)
• Non-governmental: 18,000 SEK (24 hrs, 09.00-09.00 the next day)

Instrument booking

Instrument bookings and invoicing are made online through Cryo-EM Uppsala iLab Booking System.

Acknowledgement of use

Please acknowledge any usage of the facility for sample preparation, grid screening, data collection, data processing or consultations when disseminating your results and report any publications to the facilty at cryoem-icm@uu.se.

For [include the services used here], we acknowledge the use of the Cryo-EM Uppsala facility, funded by the Department of Cell and Molecular Biology, and by the Disciplinary Domains of Science and Technology and of Medicine and Pharmacy at Uppsala University.

​SLACK

Find us on: cryo-emuppsala.slack.com (please contact us for an invitation)

Last modified: 2025-11-27