Peter Lindblad
Professor vid Institutionen för kemi - Ångström; Molekylär biomimetik; Mikrobiell Kemi
- Telefon:
- 018-471 28 26
- Mobiltelefon:
- 070-425 04 98
- E-post:
- peter.lindblad@kemi.uu.se
- Besöksadress:
- Ångströmlaboratoriet, Lägerhyddsvägen 1
75120 Uppsala - Postadress:
- Box 523
75120 Uppsala
- Akademiska meriter:
- FD, docent, prof.
- CV:
- Ladda ned CV
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Kort presentation
Professor, Microbial chemistry. Research in my group is focused on the design, engineering, construction and analyses of photosynthetic microbial cell factories (cyanobacteria) for the direct production of solar chemicals and fuels from CO2.
Biografi
My research explores the conversion of solar energy into a biofuel, focussing on micro-algal based H2-production/hydrogenases, as well as carbon-containing solar fuels, at applied, physiological, biochemical and molecular levels - in total > 150 scientific publications. Different molecular and genetic techniques are used to address transcriptional regulation and regulatory mechanisms. In the last years, his research group has developed a strong interest for Synthetic Biology and the possibilities to custom design and engineer microbial cells to carry out novel pathways and functions. Subsequent activities include proteomics and metabolomics - Systems biology - analyses of the constructed cells, followed by further re-design and re-engineering.
I am leading one of the research groups in the Swedish program on Natural and Artificial Photosynthesis, funded by the KA Wallenberg Foundation, "Molecular Solar Energy Science - MoSES", 2012-16) and the Swedish Energy Agency. I am also the coordinator of the EU/FP7-Energy project CyanoFactory (at present in negotiation phase) with 10 partners in 7 countries and participates in the Nordic collaborative project AquaFeed (2012-15). Previously, I was coordinating two Nordic collaborative projects on BioHydrogen (2005-07, 2008-11), was the Deputy coordinator of the EU/FP6/7-NEST/Energy projects Solar-H & SolarH2 (2005-07, 2008-12), participated in the EU/FP7-NEST project BiomodularH2 (2009-11) and the international project BioCO2 (2009-12). 1999-2005 I was was the coordinator of the international network Photobiological hydrogen production, Annex 15 within the International Energy Agency and presently I participates in the follow-up IEA H2 Agreement Annex 21 BioHydrogen.
Publikationer
Urval av publikationer
- Enhanced growth at low light intensity in the cyanobacterium Synechocystis PCC 6803 by overexpressing phosphoenolpyruvate carboxylase (2016)
- Enhancement of poly-3-hydroxybutyrate production in Synechocystis sp PCC 6803 by overexpression of its native biosynthetic genes (2016)
- Effects of overexpressing photosynthetic carbon flux control enzymes in the cyanobacterium Synechocystis PCC 6803 (2016)
- Turning around the electron flow in an uptake hydrogenase. EPR spectroscopy and in vivo activity of a designed mutant in HupSL from Nostoc punctiforme (2016)
- Mechanisms of carbon fixation and engineering for increased carbon fixation in cyanobacteria (2015)
- Cyanobacterial Hydrogenases and Hydrogen Metabolism Revisited: (2015)
- Hydrogen production by the engineered cyanobacterial strain Nostoc PCC 7120 Delta hupW examined in a flat panel photobioreactor system (2015)
- Design and analysis of LacI-repressed promoters and DNA-looping in a cyanobacterium (2014)
- Engineered transcriptional systems for cyanobacterial biotechnology (2014)
- Wide-dynamic-range promoters engineered for cyanobacteria (2013)
- Isolation and Characterization of the Small Subunit of the Uptake Hydrogenase from the Cyanobacterium Nostoc punctiforme. (2013)
- Potential for green microalgae to produce hydrogen, pharmaceuticals and other high value products in a combined process (2013)
- Design, Engineering, and Construction of Photosynthetic Microbial Cell Factories for Renewable Solar Fuel Production (2012)
- Synthetic Biology in Cyanobacteria (2011)
- Design and characterization of molecular tools for a Synthetic Biology approach towards developing cyanobacterial biotechnology (2010)
Senaste publikationer
- Genome Engineering by RNA-Guided Transposition for Anabaena sp. PCC 7120 (2024)
- Harnessing photosynthetic microorganisms for enhanced bioremediation of microplastics (2024)
- Recent developments in the production and utilization of photosynthetic microorganisms for food applications (2023)
- Improved lipid production and component of mycosporine-like amino acids by co-overexpression of amt1 and aroB genes in Synechocystis sp. PCC6803 (2023)
- Overexpressing Carotenoid Biosynthetic Genes in Synechocystis sp. PCC 6803 Improved Intracellular Pigments and Antioxidant Activity, Which Can Decrease the Viability and Proliferation of Lung Cancer Cells In Vitro (2023)
Alla publikationer
Artiklar
- Genome Engineering by RNA-Guided Transposition for Anabaena sp. PCC 7120 (2024)
- Harnessing photosynthetic microorganisms for enhanced bioremediation of microplastics (2024)
- Recent developments in the production and utilization of photosynthetic microorganisms for food applications (2023)
- Improved lipid production and component of mycosporine-like amino acids by co-overexpression of amt1 and aroB genes in Synechocystis sp. PCC6803 (2023)
- Overexpressing Carotenoid Biosynthetic Genes in Synechocystis sp. PCC 6803 Improved Intracellular Pigments and Antioxidant Activity, Which Can Decrease the Viability and Proliferation of Lung Cancer Cells In Vitro (2023)
- Novel concepts and engineering strategies for heterologous expression of efficient hydrogenases in photosynthetic microorganisms (2023)
- Sustainable production of photosynthetic isobutanol and 3-methyl-1-butanol in the cyanobacterium Synechocystis sp. PCC 6803 (2023)
- Enhanced productivity of extracellular free fatty acids by gene disruptions of acyl-ACP synthetase and S-layer protein in Synechocystis sp. PCC 6803 (2022)
- Anchoring the ethylene-forming enzyme to photosystem-related proteins to improve ethylene production in engineered Synechocystis PCC 6803 (2022)
- Current advances in engineering cyanobacteria and their applications for photosynthetic butanol production (2022)
- Current strategies and future perspectives in biological hydrogen production (2022)
- A combined photobiological-photochemical route to C10 cycloalkane jet fuels from carbon dioxide via isoprene (2022)
- Differential catalase activity and tolerance to hydrogen peroxide in the filamentous cyanobacteria Nostoc punctiforme ATCC 29113 and Anabaena sp. PCC 7120 (2022)
- Expressing 2-keto acid pathway enzymes significantly increases photosynthetic isobutanol production (2022)
- Enhancing microalgal lipid accumulation for biofuel production (2022)
- NordAqua, a Nordic Center of Excellence to develop an algae-based photosynthetic production platform (2021)
- Production of succinate by engineered strains of Synechocystis PCC 6803 overexpressing phosphoenolpyruvate carboxylase and a glyoxylate shunt (2021)
- Overexpression of lipA or glpD_RuBisCO in the Synechocystis sp. PCC 6803 Mutant Lacking the Aas Gene Enhances Free Fatty-Acid Secretion and Intracellular Lipid Accumulation (2021)
- Biotechnological Production of the Sunscreen Pigment Scytonemin in Cyanobacteria (2021)
- Photoautotrophic production of renewable ethylene by engineered cyanobacteria (2021)
- Photosynthetic hydrogen production: Novel protocols, promising engineering approaches and application of semi‐synthetic hydrogenases (2021)
- Engineered cyanobacteria with additional overexpression of selected Calvin-Benson-Bassham enzymes show further increased ethanol production (2021)
- Doing synthetic biology with photosynthetic microorganisms (2021)
- Semisynthetic [FeFe]-hydrogenase with stable expression and H2 production capacity in a photosynthetic microbe (2021)
- Engineering Biocatalytic Solar Fuel Production (2021)
- Hints at the Applicability of Microalgae and Cyanobacteria for the Biodegradation of Plastics (2020)
- Oligomerization and characteristics of phosphoenolpyruvate carboxylase in Synechococcus PCC 7002 (2020)
- Increased ethylene production by overexpressing phosphoenolpyruvate carboxylase in the cyanobacterium Synechocystis PCC 6803 (2020)
- Synechocystis sp. PCC 6803 overexpressing genes involved in CBB cycle and free fatty acid cycling enhances the significant levels of intracellular lipids and secreted free fatty acids (2020)
- Enhanced H 2 production with efficient N 2-fixation by fructose mixotrophically grown Anabaena sp. PCC 7120 strain disrupted in uptake hydrogenase (2020)
- Improved biohydrogen production by immobilized cells of the green algaTetrasporasp. CU2551 incubated under aerobic condition (2020)
- Current processes and future challenges of photoautotrophic production of acetyl-CoA-derived solar fuels and chemicals in cyanobacteria (2020)
- Membrane-Inlet Mass Spectrometry Enables a Quantitative Understanding of Inorganic Carbon Uptake Flux and Carbon Concentrating Mechanisms in Metabolically Engineered Cyanobacteria (2019)
- Improved lipid production via fatty acid biosynthesis and free fatty acid recycling in engineered Synechocystis sp. PCC 6803 (2019)
- Combining isotopically non-stationary metabolic flux analysis with proteomics to unravel the regulation of the Calvin-Benson-Bassham cycle in Synechocystis sp. PCC 6803 (2019)
- Building an Inducible T7 RNA Polymerase/T7 Promoter Circuit in Synechocystis sp. PCC6803 (2019)
- Multifaceted biofuel production by microalgal isolates from Pakistan (2019)
- CyanoFactory, a European consortium to develop technologies needed to advance cyanobacteria as chassis for production of chemicals and fuels (2019)
- Modular engineering for efficient photosynthetic biosynthesis of 1-butanol from CO2 in cyanobacteria (2019)
- Improvement of photobiological hydrogen production by suspended and immobilized cells of the N-2-fixing cyanobacterium Fischerella muscicola TISTR 8215 (2019)
- Glycogen Production in Marine Cyanobacterial Strain Synechococcus sp NKBG 15041c (2018)
- Engineered cyanobacteria with enhanced growth show increased ethanol production and higher biofuel to biomass ratio (2018)
- Engineering photoautotrophic carbon fixation for enhanced growth and productivity (2018)
- Sustainable photobiological hydrogen production via protein engineering of cyanobacterial hydrogenases (2018)
- Enhanced hydrogen production by optimization of immobilized cells of the green alga Tetraspora sp CU2551 grown under anaerobic condition (2018)
- Protein engineering of α-ketoisovalerate decarboxylase for improved isobutanol production in Synechocystis PCC 6803 (2018)
- Enhancement of photosynthetic isobutanol production in engineered cells of Synechocystis PCC 6803 (2018)
- Synechocystis PCC 6803 cells heterologously expressing bacterial tyrosine ammonia lyase can use exogenous tyrosine for p-coumaric acid production (2018)
- Co-overexpression of native phospholipid-biosynthetic genes plsX and plsC enhances lipid production in Synechocystis sp PCC 6803 (2018)
- Generation of a functional, semisynthetic [FeFe]-hydrogenase in a photosynthetic microorganism (2018)
- In vivo activation of an [FeFe] hydrogenase using synthetic cofactors (2017)
- Microalgal hydrogen production (2017)
- Synechocystis PCC 6803 overexpressing RuBisCO grow faster with increased photosynthesis (2017)
- Isobutanol production in Synechocystis PCC 6803 using heterologous and endogenous alcohol dehydrogenases (2017)
- Sll1783, a monooxygenase associated with polysaccharide processing in the unicellular cyanobacterium Synechocystis PCC 6803 (2017)
- Enhanced growth at low light intensity in the cyanobacterium Synechocystis PCC 6803 by overexpressing phosphoenolpyruvate carboxylase (2016)
- Enhancement of poly-3-hydroxybutyrate production in Synechocystis sp PCC 6803 by overexpression of its native biosynthetic genes (2016)
- Effects of overexpressing photosynthetic carbon flux control enzymes in the cyanobacterium Synechocystis PCC 6803 (2016)
- Turning around the electron flow in an uptake hydrogenase. EPR spectroscopy and in vivo activity of a designed mutant in HupSL from Nostoc punctiforme† (2016)
- Turning around the electron flow in an uptake hydrogenase. EPR spectroscopy and in vivo activity of a designed mutant in HupSL from Nostoc punctiforme (2016)
- Turning around the electron flow in an uptake hydrogenase. EPR spectroscopy and in vivo activity of a designed mutant in HupSL from Nostoc punctiforme (2016)
- Potential of Synechocystis PCC 6803 as a novel cyanobacterial chassis for heterologous expression of enzymes in the trans-resveratrol biosynthetic pathway (2016)
- Emerging technologies illuminate facets of photosynthesis in cyanobacteria (2015)
- Mechanisms of carbon fixation and engineering for increased carbon fixation in cyanobacteria (2015)
- Analysis of the leakage of gene repression by an artificial TetR-regulated promoter in cyanobacteria (2015)
- Cyanobacterial Hydrogenases and Hydrogen Metabolism Revisited: (2015)
- Molecular Cloning and Biochemical Characterization of the Iron Superoxide Dismutase from the Cyanobacterium Nostoc punctiforme ATCC 29133 and Its Response to Methyl Viologen-Induced Oxidative Stress (2015)
- Hydrogen production by the engineered cyanobacterial strain Nostoc PCC 7120 Delta hupW examined in a flat panel photobioreactor system (2015)
- Photoinduced reduction of the medial FeS center in the hydrogenase small subunit HupS from Nostoc punctiforme (2015)
- Cyanobacteria as a source of hydrogen for methane formation (2014)
- Design and analysis of LacI-repressed promoters and DNA-looping in a cyanobacterium (2014)
- Engineered transcriptional systems for cyanobacterial biotechnology (2014)
- Characterization of ten H-2 producing cyanobacteria isolated from the Baltic Sea and Finnish lakes (2014)
- A high constitutive catalase activity confers resistance to methyl viologen-promoted oxidative stress in a mutant of the cyanobacterium Nostoc punctiforme ATCC 29133 (2014)
- Evidence for non-cysteinyl coordination of the iron-sulfur clusters of HupS, the small subunit of the uptake hydrogenase from Nostoc punctiforme (2014)
- Wide-dynamic-range promoters engineered for cyanobacteria (2013)
- Metabolic and genetic engineering of cyanobacteria for enhanced hydrogen production (2013)
- Isolation and Characterization of the Small Subunit of the Uptake Hydrogenase from the Cyanobacterium Nostoc punctiforme. (2013)
- Potential for green microalgae to produce hydrogen, pharmaceuticals and other high value products in a combined process (2013)
- CRISPR in the extended hyp-operon of the cyanobacterium Nostoc sp. strain PCC 7120, characteristics and putative function(s) (2012)
- Inactivation of uptake hydrogenase leads to enhanced and sustained hydrogen production with high nitrogenase activity under high light exposure in the cyanobacterium Anabaena siamensis TISTR 8012 (2012)
- Redirecting the electron flow towards the nitrogenase and bidirectional Hox-hydrogenase by using specific inhibitors results in enhanced H-2 production in the cyanobacterium Anabaena siamensis TISTR 8012 (2012)
- Editorial - Photosynthetic microorganisms for bio-fuel production (2012)
- HupW Protease Specifically Required for Processing of the Catalytic Subunit of the Uptake Hydrogenase in the Cyanobacterium Nostoc sp Strain PCC 7120 (2012)
- Design, Engineering, and Construction of Photosynthetic Microbial Cell Factories for Renewable Solar Fuel Production (2012)
- Sulfate permease (SulP) and hydrogenase (HydA) in the green alga Tetraspora sp CU2551 (2012)
- Increased H(2) production in the cyanobacterium Synechocystis sp strain PCC 6803 by redirecting the electron supply via genetic engineering of the nitrate assimilation pathway (2011)
- Na(+)-stimulated nitrate uptake with increased activity under osmotic upshift in Synechocystis sp strain PCC 6803 (2011)
- A HupS-GFP fusion protein demonstrates a heterocyst-specific localization of the uptake hydrogenase in Nostoc punctiforme (2011)
- Genetically engineered light sensors for control of bacterial gene expression (2011)
- Synthetic Biology in Cyanobacteria (2011)
- Transcript analysis of the extended hyp-operons in the cyanobacteria Nostoc sp. strain PCC 7120 and Nostoc punctiforme ATCC 29133 (2011)
- Development of suitable photobioreactors for CO2 sequestration addressing global warming using green algae and cyanobacteria (2011)
- FtsZ degradation in the cyanobacterium Anabaena sp. strain PCC 7120 (2011)
- Novel Insights into the Regulation of LexA in the Cyanobacterium Synechocystis sp Strain PCC 6803 (2011)
- The CyAbrB transcription factor CalA regulates the iron superoxide dismutase in Nostoc sp. strain PCC 7120 (2010)
- CalA, a cyanobacterial AbrB protein, interacts with the upstream region of hypC and acts as a repressor of its transcription in the cyanobacterium Nostoc sp. strain PCC 7120 (2010)
- Response of H-2 production and Hox-hydrogenase activity to external factors in the unicellular cyanobacterium Synechocystis sp strain PCC 6803 (2010)
- Recent trends on the development of photobiological processes and photobioreactors for the improvement of hydrogen production (2010)
- Design and characterization of molecular tools for a Synthetic Biology approach towards developing cyanobacterial biotechnology (2010)
- Enhanced biohydrogen production by the N-2-fixing cyanobacterium Anabaena siamensis strain TISTR 8012 (2010)
- A newly isolated green alga, Tetraspora sp. CU2551, from Thailand with efficient hydrogen production (2010)
- Evidence for transcription of three genes with characteristics of hydrogenases in the green alga Chlamydomonas noctigama (2010)
- Energy biotechnology with cyanobacteria (2009)
- Electron transfer protein complexes in the thylakoid membranes of heterocysts from the cyanobacterium Nostoc punctiforme (2009)
- Diversity and transcription of proteases involved in the maturation of hydrogenases in Nostoc punctiforme ATCC 29133 and Nostoc sp strain PCC 7120 (2009)
- Characterization of the hupSL promoter activity in Nostoc punctiforme ATCC 29133 (2009)
- Characterization of the hupSL promoter activity in Nostoc punctiforme ATCC 29133 (2009)
- Biomimetic and Microbial Approaches to Solar Fuel Generation (2009)
- Transcriptional regulation of the cyanobacterial bidirectional Hox-hydrogenase (2009)
- Quantitative overview of N2 fixation in Nostoc punctiforme ATCC 29133 through cellular enrichments and iTRAQ shotgun proteomics (2009)
- A guide for in-house design of template-switch-based 5' rapid amplification of cDNA ends systems (2009)
- Analysis of current and alternative phenol based RNA extraction methodologies for cyanobacteria (2009)
- Transcription of the extended hyp-operon in Nostoc sp. strain PCC 7120 (2008)
- Very small mobile repeated elements in cyanobacterial genomes (2008)
- An AbrB-Like Protein Regulates the Expression of the Bidirectional Hydrogenase in Synechocystis sp. Strain PCC 6803. (2008)
- Quantitative shotgun proteomics of enriched heterocysts from Nostoc sp. PCC 7120 using 8-Plex isobaric peptide tags (2008)
- H2 production from marine and freshwater species of green algae during sulfur deprivation and considerations for bioreactor design (2008)
- Isolation and characterization of thylakoid membranes from the filamentous cyanobacterium Nostoc punctiforme (2007)
- Transcription and regulation of the hydrogenase(s) accessory genes, hypFCDEAB, in the cyanobacterium Lyngbya majuscula CCAP 1446/4 (2007)
- Webtag (2007)
- Transcription and Regulation of the Bidirectional Hydrogenase in the Cyanobacterium Nostoc sp. Strain PCC 7120 (2007)
- BioCO2 (2007)
- An iTRAQ-based quantitative analysis to elaborate the proteomic response of Nostoc sp PCC 7120 under N-2 fixing conditions (2007)
- Cyanobacterial hydrogenases (2007)
- Experimental and bioinformatic approaches for analyzing and visualizing cyanobacterial nitrogen and hydrogen metabolism (2007)
- The bidirectional hydrogenase in the cyanobacterium Synechocystis sp. strain PCC 6803 (2006)
- Generation of non-genomic oligonucleotide tag sequences for RNA template-specific PCR (2006)
- Production of H2 by sulphur-deprived cells of the unicellular cyanobacteria Gloeocapsa alpicola and Synechocystis sp. PCC 6803 during dark incubation with methane or a various extracellular pH (2005)
- Heterocyst-Specific Excision of the Anabaena sp. Strain PCC 7120 hupL Element requires xisC (2005)
- LexA, a transcription regulator binding in the promoter region of the bidirectional hydrogenase in the cyanobacterium sp. PCC 6803 (2005)
- LexA, a transcription regulator binding in the promoter region of the bidirectional hydrogenase in the cyanobacterium Synechocystis sp. PCC 6803 (2005)
- Sequenced based data supports a single Nostoc strain in coralloid roots of cycads (2004)
- Sequence based data supports a single Nostoc strain in individual coralloid roots of cycads (2004)
- Gas Exchange in the Filamentous Cyanobacterium Nostoc punctiforme Strain ATCC 29133 and Its Hydrogenase-Deficient Mutant Strain NHM5 (2004)
- Cyanobacterial H2 production - a comparative analysis (2004)
- Cyanobacterial H2 production — a comparative analysis (2004)
- Transcriptional Regulation of Nostoc Hydrogenases: Effects of Hydrogen, Oxygen and Nickel. (2002)
- The cyanobacterial tRNA(Leu) (UAA) intron: Evolutionary patterns in a genetic marker (2002)
- A hydrogen-producing, hydrogenase-free mutant strain of Nostoc punctiforme ATCC 29133 (2002)
- Photoproduction of H2 by wildtype Anabaena PCC 7120 and a hydrogen uptake deficient mutant: From laboratory experiments to outdoor culture (2002)
- Fascilin Domain Proteins are present in Nostoc Symbionts of Lichens. (2002)
- Hydrogenases and Hydrogen Metabolism of Cyanobacteria (2002)
- Genetic diversity of Nostoc symbionts endophytically associated with two bryophyte species (2001)
- Cloning and characterization of a hyp gene cluster in the filamentous cyanobacterium Nostoc sp. strain PCC 73102 (2001)
- hupS and hupL constitute a transcription unit in the cyanobacterium Nostoc sp. PCC 73102 (2000)
- Cyanobiont diversity within coralloid roots of selected cycad species (1999)
- A HupS-GFP fusion protein demonstrates a heterocyst specific localisation of the uptake hydrogenase in the cyanobacterium Nostoc punctiforme
- Gas exchange in the filamentous cyanobacterium Nostoc punctiforme strain ATCC 29133 and its hydrogenase deficient mutant strain NHM5
- Metabolic engineering of Synechocystis PCC 6803 for photosynthetic 1-butanol production
- [FeFe]-hydrogenase synthetically activated in the filamentous cyanobacterium Nostoc punctiforme ATCC 29133
- Directed evolution of α-ketoisovalerate decarboxylase for improved isobutanol and 3-methyl-1-butanol production in cyanobacteria