Drug Delivery

Our research is like an exciting journey through the human body, where we explore how administered drugs are absorbed and travel through our body to reach where they are needed. We study both traditional small drug molecules and biological drugs such as peptides and oligonucleotides. By understanding how drugs are absorbed, distributed between different parts of the body, metabolized, and excreted at the molecular and cellular level, we create the conditions for the development of more effective drugs and drug formulations. Our research is the most cited at our faculty and has resulted in many international awards.
We combine various disciplines such as bioinformatics, cell and molecular biology, -omics (a term that covers different types of 'omics', such as genomics, proteomics, metabolomics), with computational chemistry, pharmacokinetics, and pharmacy. Instead of using experimental animals, we develop cell models from human tissues such as the intestine, liver, and kidney. We culture the cells both as cell monolayers (2D format) and as organoids (3D format) and use them, among other things, to study how important so-called transport proteins and drug-metabolizing proteins affect the metabolism of drugs in our bodies. We use stem cells as human cells in our research. Important methods in our toolbox, in addition to our cell models, include mass spectrometry-based proteomics where we can analyze the presence of thousands of proteins, how the proteins change in our cells, and which proteins a particular drug binds to. We then use the results to optimize physiologically based pharmacokinetic (PBPK) models so that we can predict the behavior of drugs in our bodies over time. Recently, our techniques have also begun to be used in chemical testing, such as endocrine-disrupting substances, pesticides, and toxins.
Our models are used by the pharmaceutical industry and academic researchers worldwide and also contribute daily to the research within the Drug Discovery platform at SciLifeLab in the form of the Department of Pharmacy's infrastructure unit UDOPP. There, we analyze the properties of various drug candidates and predict how they will behave in the body before they are even tested in the laboratory or in clinical studies. This allows us to optimize the properties of drug candidates and develop better drugs. In summary, our research aims to increase our understanding of how drugs work in the body and to open doors to new and more effective ways to deliver them to the places where they are needed most. It is an exciting journey, and we look forward to seeing where it takes us next.
Publications
Part of Biochimica et Biophysica Acta - Molecular Basis of Disease, 2025
- DOI for Mild-to-moderate psoriasis is associated with subclinical inflammation in the duodenum and a tendency of disturbed intestinal barrier
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Part of Clinical Pharmacokinetics, p. 109-120, 2024
- DOI for Digoxin Pharmacokinetics in Patients with Obesity Before and After a Gastric Bypass or a Strict Diet Compared with Normal Weight Individuals
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Part of International Journal of Pharmaceutics, 2024
Global 13C tracing and metabolic flux analysis of intact human liver tissue ex vivo
Part of Nature Metabolism, 2024
- DOI for Global 13C tracing and metabolic flux analysis of intact human liver tissue ex vivo
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Illuminating the function of the orphan transporter, SLC22A10, in humans and other primates
Part of Nature Communications, 2024
- DOI for Illuminating the function of the orphan transporter, SLC22A10, in humans and other primates
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Part of British Journal of Pharmacology, 2024
Part of EBioMedicine, 2024
- DOI for Loss of heterozygosity of CYP2D6 enhances the sensitivity of hepatocellular carcinomas to talazoparib
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OCT1 (SLC22A1) transporter kinetics and regulation in primary human hepatocyte 3D spheroids
Part of Scientific Reports, 2024
- DOI for OCT1 (SLC22A1) transporter kinetics and regulation in primary human hepatocyte 3D spheroids
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2024
- DOI for Proteomic Profiling Reveals Age-Related Changes in Transporter Proteins in the Human Blood-Brain Barrier
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Part of Biotechnology Journal, 2024
- DOI for The choice of ultra-low attachment plates impacts primary human and primary canine hepatocyte spheroid formation, phenotypes, and function
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Epithelial and microbial determinants of colonic drug distribution
Part of European Journal of Pharmaceutical Sciences, 2023
- DOI for Epithelial and microbial determinants of colonic drug distribution
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Part of Clinical Pharmacokinetics, p. 725-735, 2023
- DOI for Neither Gastric Bypass Surgery Nor Diet-Induced Weight-Loss Affect OATP1B1 Activity as Measured by Rosuvastatin Oral Clearance
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Part of Computational and Structural Biotechnology Journal, p. 4361-4369, 2023
- DOI for Proteome deconvolution of liver biopsies reveals hepatic cell composition as an important marker of fibrosis
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Part of ACS Nano, p. 14210-14229, 2022
- DOI for Barriers to the Intestinal Absorption of Four Insulin-Loaded Arginine-Rich Nanoparticles in Human and Rat
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Part of European Journal of Clinical Pharmacology, p. 1289-1299, 2022
- DOI for Correlations between 4 beta-hydroxycholesterol and hepatic and intestinal CYP3A4: protein expression, microsomal ex vivo activity, and in vivo activity in patients with a wide body weight range
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Drug Disposition Protein Quantification in Matched Human Jejunum and Liver From Donors With Obesity
Part of Clinical Pharmacology and Therapeutics, p. 1142-1154, 2022
- DOI for Drug Disposition Protein Quantification in Matched Human Jejunum and Liver From Donors With Obesity
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Part of International Journal of Molecular Sciences, 2022
- DOI for Hepatic Expression of the Na+-Taurocholate Cotransporting Polypeptide Is Independent from Genetic Variation
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Part of Pharmaceutical research, p. 1599-1613, 2022
- DOI for High Throughput Screening of a Prescription Drug Library for Inhibitors of Organic Cation Transporter 3, OCT3
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Part of Clinical and Translational Science, p. 2685-2696, 2022
- DOI for Impact of type 2 diabetes on in vivo activities and protein expressions of cytochrome P450 in patients with obesity
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Part of Biomedicine and Pharmacotherapy, 2022
- DOI for Inhibition of prolyl oligopeptidase: A promising pathway to prevent the progression of age-related macular degeneration
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Part of Nucleic Acids Research, 2022
- DOI for Monitoring drug–target interactions through target engagement-mediated amplification on arrays and in situ
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Professor Margareta Hammarlund-Udenaes - Humbled Scientist Shaping Modern Neuropharmacokinetics
Part of Pharmaceutical research, p. 1283-1289, 2022
Part of British Journal of Clinical Pharmacology, p. 4121-4133, 2022
- DOI for Short- and long-term effects of body weight, calorie restriction and gastric bypass on CYP1A2, CYP2C19 and CYP2C9 activity
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Part of Clinical and Translational Science, p. 221-233, 2022
- DOI for Short- and long-term effects of body weight loss following calorie restriction and gastric bypass on CYP3A-activity: a non-randomized three-armed controlled trial
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Part of International Journal of Pharmaceutics, 2022
Part of Journal of Experimental & Clinical Cancer Research, 2021
- DOI for Common and mutation specific phenotypes of KRAS and BRAF mutations in colorectal cancer cells revealed by integrative -omics analysis
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Conditions for maintenance of hepatocyte differentiation and function in 3D cultures
Part of iScience, 2021
- DOI for Conditions for maintenance of hepatocyte differentiation and function in 3D cultures
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Part of Advanced Drug Delivery Reviews, p. 289-331, 2021
- DOI for Current challenges and future perspectives in oral absorption research: An opinion of the UNGAP network
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Part of Journal of Pharmaceutical Sciences, p. 388-396, 2021
- DOI for Expanding the Efflux In Vitro Assay Toolbox: A CRISPR-Cas9 Edited MDCK Cell Line with Human BCRP and Completely Lacking Canine MDR1
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Part of Nature Materials, p. 1043-1044, 2021
Hepatocyte size fractionation allows dissection of human liver zonation
Part of Journal of Cellular Physiology, p. 5885-5894, 2021
- DOI for Hepatocyte size fractionation allows dissection of human liver zonation
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Part of Molecular Pharmaceutics, p. 1792-1805, 2021
- DOI for Influence of Proteome Profiles and Intracellular Drug Exposure on Differences in CYP Activity in Donor-Matched Human Liver Microsomes and Hepatocytes
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Newly developed dual topoisomerase inhibitor P8-D6 is highly active in ovarian cancer
Part of THERAPEUTIC ADVANCES IN MEDICAL ONCOLOGY, 2021
- DOI for Newly developed dual topoisomerase inhibitor P8-D6 is highly active in ovarian cancer
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Part of Journal of Pharmaceutical Sciences, p. 239-250, 2021
- DOI for Proteomics-Informed Identification of Luminal Targets For In Situ Diagnosis of Inflammatory Bowel Disease
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Part of Clinical Pharmacology and Therapeutics, p. 762-771, 2021
- DOI for Proteomics‐Informed Prediction of Rosuvastatin Plasma Profiles in Patients with a Wide Range of Body Weight
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Part of Drug Metabolism And Disposition, p. 8-17, 2020
A new opening for orally taken peptide drugs
Part of Nature Biomedical Engineering, p. 12-13, 2020
Part of Pharmaceutics, 2020
- DOI for Acamprosate Is a Substrate of the Human Organic Anion Transporter (OAT) 1 without OAT3 Inhibitory Properties: Implications for Renal Acamprosate Secretion and Drug-Drug Interactions
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Cell-type-resolved proteomic analysis of the human liver
Part of Liver international, p. 1770-1780, 2020
- DOI for Cell-type-resolved proteomic analysis of the human liver
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Exploiting loss of heterozygosity for allele-selective colorectal cancer chemotherapy
Part of Nature Communications, 2020
- DOI for Exploiting loss of heterozygosity for allele-selective colorectal cancer chemotherapy
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Global variability analysis of mRNA and protein concentrations across and within human tissues
Part of NAR Genomics and Bioinformatics, 2020
- DOI for Global variability analysis of mRNA and protein concentrations across and within human tissues
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The Critical Role of Passive Permeability in Designing Successful Drugs
Part of ChemMedChem, p. 1862-1874, 2020
Part of Pharmaceutical research, 2019
- DOI for A Cell-Free Approach Based on Phospholipid Characterization for Determination of the Cell Specific Unbound Drug Fraction (f(u,cell))
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A simple approach for restoration of differentiation and function in cryopreserved human hepatocytes
Part of Archives of Toxicology, p. 819-829, 2019
- DOI for A simple approach for restoration of differentiation and function in cryopreserved human hepatocytes
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Comprehensive drug uptake transporter proteomics and metabolomic profiling of human liver
Part of Naunyn-Schmiedeberg's Archives of Pharmacology, 2019
Experimental approaches to understand intracellular based drug bioavailability
Part of Drug Metabolism and Pharmacokinetics, 2019
Image-based quantification of cell debris as a measure of apoptosis
Part of Analytical Chemistry, p. 5548-5552, 2019
- DOI for Image-based quantification of cell debris as a measure of apoptosis
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Impact of Intracellular Concentrations on Metabolic Drug-Drug Interaction Studies
Part of AAPS Journal, 2019
- DOI for Impact of Intracellular Concentrations on Metabolic Drug-Drug Interaction Studies
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Part of Scientific Reports, 2019
- DOI for Improved predictions of time-dependent drug-drug interactions by determination of cytosolic drug concentrations
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Part of Journal of Proteome Research, p. 217-224, 2019
- DOI for Multiple-Enzyme-Digestion Strategy Improves Accuracy and Sensitivity of Label- and Standard-Free Absolute Quantification to a Level That Is Achievable by Analysis with Stable Isotope-Labeled Standard Spiking
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Contact
- Visiting Address: BMC, Husargatan 3, A1:2, A2:2, A3:3, B3:3, B3:4, C2:2
- Letter and Postal Address: Box 580, SE-751 23 Uppsala