Maria Tenje
Professor at Department of Materials Science and Engineering; Biomedical Engineering
- Telephone:
- +46 18 471 70 61
- E-mail:
- maria.tenje@angstrom.uu.se
- Visiting address:
- Ångströmlaboratoriet, Lägerhyddsvägen 1
- Postal address:
- Box 35
751 03 UPPSALA
- ORCID:
- 0000-0002-1264-1337
More information is available to staff who log in.
Short presentation
I lead the research group EMBLA with the ambition to develop miniaturised systems for biomedical and life science applications based on advanced micro and nanofabrication methods and the use of microfluidics.
As of July 2021, I co-head the Division of Biomedical Engineering and since January 2024 I am the Director of the Uppsala MedTech Science and Innovation Centre (MTSI).
I had a research exchange at UC Santa Barbara during the 2022/2023 academic year.
Keywords
- bioacoustics
- biomedical engineering
- droplet microfluidics
- leadership
- med-tech
- microfabrication
- organ-on-chip
- scilifelab
Biography
I joined Uppsala University as Associate Senior Lecturer in September 2014 upon an open call across the Faculty to attract young researchers to Uppsala. In early 2016 I was promoted to Senior Lecturer and as of 2019 I am full Professer and lead the research group EMBLA (Enabling Microtechnologies for Biomedical and Life science Applications) and teach in the undergradute programme for Engineering Physics. In July 2021 I started the new Division on Biomedical Engineering together with my colleague Prof. Cecilia Persson at the Dept. of Materials Science and Engineering that we now co-lead.
My research career started in Denmark, where I received her PhD degree in Microtechnology from the Technical University of Denmark (DTU) in 2007 after having completed my MSc degree in Physics at Lund University in 2003 and my BSc (Hons.) degree in Physics at Imperial College, London in 2002. I was appointed Docent at Uppsala University in 2015.
My main research interests are micro- and nanofabrication, microfluidic systems for on-chip cell cultures, droplet microfluidics, acoustofluidics and organs-on-chip.Publications
Recent publications
- Universal Biomaterial-on-Chip (2024)
- Size-related variability of oxygen consumption rates in individual human hepatic cells (2024)
- A perfusable multi-hydrogel vasculature on-chip engineered by 2-photon 3D printing and scaffold molding to improve microfabrication fidelity in hydrogels (2024)
- On-chip fabrication of tailored 3D hydrogel scaffolds to model cancer cell invasion and interaction with endothelial cells (2024)
- SlipO2Chip - single-cell respiration under tuneable environments (2024)
All publications
Articles
- Universal Biomaterial-on-Chip (2024)
- Size-related variability of oxygen consumption rates in individual human hepatic cells (2024)
- A perfusable multi-hydrogel vasculature on-chip engineered by 2-photon 3D printing and scaffold molding to improve microfabrication fidelity in hydrogels (2024)
- On-chip fabrication of tailored 3D hydrogel scaffolds to model cancer cell invasion and interaction with endothelial cells (2024)
- SlipO2Chip - single-cell respiration under tuneable environments (2024)
- Integration of multiple flexible electrodes for real-time detection of barrier formation with spatial resolution in a gut-on-chip system (2024)
- A Microfluidic Platform for In Situ Studies of Bacteria Electroporation (2024)
- Round-robin testing of commercial two-photon polymerization 3D printers (2023)
- Technology platform for facile handling of 3D hydrogel cell culture scaffolds (2023)
- Experimental Characterization and Mathematical Modeling of the Adsorption of Proteins and Cells on Biomimetic Hydroxyapatite (2022)
- A microfluidic-based approach to investigate the inflammatory response of macrophages to pristine and drug-loaded nanostructured hydroxyapatite (2022)
- Integrated cooling system for microfluidic PDMS devices used in biological microscopy studies (2022)
- Cell chirality exhibition of brain microvascular endothelial cells is dependent on micropattern width (2022)
- Confocal imaging dataset to assess endothelial cell orientation during extreme glucose conditions (2022)
- Microfluidics platform for studies of peptide - polyelectrolyte interaction (2022)
- Photophysiological response of Symbiodiniaceae single cells to temperature stress (2022)
- A microscopy-compatible temperature regulation system for single-cell phenotype analysis - demonstrated by thermoresponse mapping of microalgae (2021)
- A microfluidics-based method for culturing osteoblasts on biomimetic hydroxyapatite (2021)
- A microfluidic chip carrier including temperature control and perfusion system for long-term cell imaging (2021)
- Binary acoustic trapping in a glass capillary (2021)
- Acoustic focusing of beads and cells in hydrogel droplets (2021)
- In-Line Analysis of Organ-on-Chip Systems with Sensors (2021)
- Effect of the Addition Frequency of 5-Azacytidine in Both Micro- and Macroscale Cultures (2021)
- A droplet acoustofluidic platform for time-controlled microbead-based reactions (2021)
- Organ-on-a-chip technology (2021)
- Brain microvasculature endothelial cell orientation on micropatterned hydrogels is affected by glucose level variations (2021)
- Fabrication and characterisation of a silicon-borosilicate glass microfluidic device for synchrotron-based hard X-ray spectroscopy studies (2021)
- PDMS leaching and its implications for on-chip studies focusing on bone regeneration applications (2020)
- Exploring microfluidics as a tool to evaluate the biological properties of a titanium alloy under dynamic conditions (2020)
- Optimisation of the droplet split design for high acoustic particle enrichment in droplet microfluidics (2020)
- Fabrication of Silicon Microfluidic Chips for Acoustic Particle Focusing Using Direct Laser Writing (2020)
- Dynamics of DNA Clogging in Hafnium Oxide Nanopores (2020)
- A simplified approach to control cell adherence on biologically derived in vitro cell culture scaffolds by direct UV-mediated RGD linkage (2020)
- A practical guide to microfabrication and patterning of hydrogels for biomimetic cell culture scaffolds (2020)
- An acoustofluidic platform for non-contact trapping of cell-laden hydrogel droplets compatible with optical microscopy (2019)
- On-chip background dilution in droplets with high particle recovery using acoustophoresis (2019)
- Integrated thin film resistive sensors for in situ temperature measurements in an acoustic trap (2019)
- Intra-droplet acoustic particle focusing (2018)
- Binary particle separation in droplet microfluidics using acoustophoresis (2018)
- Particle Manipulation Methods in Droplet Microfluidics (2018)
- Biomimetic spinning of artificial spider silk from a chimeric minispidroin (2017)
- An intra-droplet particle switch for droplet microfluidics using bulk acoustic waves (2017)
- Fabrication of user-friendly and biomimetic 1,1′-carbonyldiimidazole cross-linked gelatin/agar microfluidic devices (2017)
- Improved positioning and detectability of microparticles in droplet microfluidics using two-dimensional acoustophoresis (2017)
- Evaluation of Biocompatibility and Release of Reactive Oxygen Species of Aluminum Oxide-Coated Materials (2016)
- Simvastatin and zinc synergistically enhance osteoblasts activityand decrease the acute response of inflammatory cells (2016)
- Dense high-aspect ratio 3D carbon pillars on interdigitated microelectrode arrays (2015)
- Controlled lateral positioning of microparticles inside droplets using acoustophoresis (2015)
- Acoustophoretic removal of proteins from blood components (2015)
- Acoustic trapping as a generic non-contact incubation site for multiplex bead-based assays (2015)
- In vitro blood-brain barrier models (2015)
- Microfluidic PMMA interfaces for rectangular glass capillaries (2014)
- In-situ monitoring of potential enhanced DNA related processes using electrochemical quartz crystal microbalance with dissipation (EQCM-D) (2014)
- Universal Biomaterial-on-Chip: A modular platform for flexible biomaterial integration and versatile quantitative assessment
- Microengineering a cell co-culture platform for glioblastoma cell migration and intravasation studies
- The effect of unidirectional and recirculating flow on the behavior of MC3T3-E1 preosteoblast-like cells in a microfluidic system
- Environment-friendly oils and surfactants for droplet microfluidics – the need to find replacements for PFAS-based chemicals
- Real-time pooled optical screening with single-cell isolation capability
- Long-term droplet cell culture enabled by droplet acoustofluidics
- Evaluation of cell chirality of brain microvascular endothelial cells
- Effects of the choice of the continuous phase in droplet microfluidics on internal particle manipulation with acoustophoresis
- Mapping the acoustic properties of two-phase systems for use in droplet acoustofluidics
- On-chip acoustic formation of levitated polystyrene bead clusters in hydrogel.
- Experimental verification of thermoviscous heating on-chip in an acoustofluidic system
- In-line TNF-α detection in organs-on-chip using acoustic trapping
- Microfluidic system with integrated nanocellulose cell culture substrate to study alignment of human umbilical vein endothelial cells in relation to external physical cues
Conferences
- Speed of sound mismatch deteriorates acoustophoresis focusing quality within droplets (2024)
- The effect of speed of sound mismatch on acoustophoretic focusing quality within droplets (2024)
- Mapping the acoustic properties of two-phase systems for use in droplet acoustofluidics (2023)
- In-line detection of TNF-α using an acoustic trap connected to an Infection-on-Chip model (2023)
- A 3D-Printed Device Providing Mechanical Strain for Matrigel-Incorprated Organoids (2022)
- Droplet Acoustofluidics and Pico-injection for Long-term Cell Culture (2022)
- A Universal Microfluidic Platform for In Vitro Biomaterial Evaluation (2022)
- 2-photon polymerization–benchmarking commercial printers to access the nanometric scale in 3D printing (2022)
- Comparison of commercial systems for two-photon polymerization direct laser writing (2022)
- Integrated optical read-out for bead-based assays in an acoustic trap (2022)
- In-line detection of TNF-α using an acoustic trap connected to an Infection-on-Chip model (2022)
- Integrating nanocellulose in a microfluidic platform for topography guided cell culture. (2022)
- Modelling adsorption of proteins and cells on biomimetic hydroxyapatite (2021)
- Influence of flow in the adhesion and proliferation of cells on hydroxyapatite integrated in a microscale culture (2021)
- Binary acoustic particle trapping in glass capillaries (2021)
- A carrier with an integrated perfusion and heating systems for long-term imaging of microfluidic chips (2021)
- A comparison of two approaches to integrate medical grade titanium on-chip (2021)
- Biomaterials-on-chip (2021)
- In-line analysis of organ-on-chip systems with sensors – an overview (2021)
- Mapping the thermoresponses of micralgae by integrating single-cell arrays on a programmable temperature stage (2021)
- Time-controlled Microbead-based Reactions in Droplets using Acoustophoresis (2021)
- Enabling off-chip analysis for in-droplet enzyme reactions using acoustophoresis (2021)
- Emulating the gut-liver axis (2021)
- Acoustofluidic Method to Align Polystyrene Beads and Cells in Hydrogels (2021)
- Evaluation of biocompatibility of acousticfocusing of cells in hydrogel droplets (2021)
- Controlling the acoustic contrast factor of Agarose droplets (2021)
- Fabrication of electrodes on flexible substrates for OoC integration (2021)
- Combined effects of thermal stress and UV filter exposure on single cells of Symbiodinium (2021)
- Quantitative evaluation of osteoblast proliferation and differentiation on a biomaterial in a microfluidic device (2020)
- Hydrogel membrane fabricated by 2-photon polymerization in a microfluidic chip for bio-interface investigations (2020)
- On-chip evaluation of the biological properties of medical-grade titanium (2020)
- Increasing the physiological relevance of the gut-on-chip model (2020)
- Free-hanging hydrogel structures as more in vivo-like barrier model (2020)
- Alignment of brain endothelial cells on patterned hyaluronic acid hydrogels (2020)
- Evaluation of Ionic Interactions of Bone Cement-on-Chip (2019)
- Development and validation of a reusable microfluidic system for the evaluation of biomaterials’ biological properties (2019)
- Medical grade titanium on-chip (2019)
- Trapping of Cell-Laden Hyaluronic Acid-Acrylamide Hydrogel Droplets using Bulk Acoustic Waves (2019)
- Acoustic trapping (2019)
- Improved acoustic particle enrichment in droplets by optimising the droplet split design (2019)
- Biomimetic spider silk production on a microfluidic chip (2019)
- Microfluidic chip for large-scale phenotypic characterization of E. coli (2019)
- A continuous on-chip droplet washing platform with high bead recovery by acoustofluidics (2019)
- Droplet Dilution Unit Operation Including Bead Washing Using Integrated Acoustophoresis (2019)
- Micro engineering of organ-on-chip systems (2019)
- Replacing animal testing with organs-on-chip (2019)
- Microengineering of hydrogels for organ-on-chip applications (2019)
- Acoustic particle manipulation in droplet microfluidics (2019)
- Integrated thin film resistive sensors for in situ temperature measurements in an acoustic trap (2019)
- Bone Cement Embedded in a Microfluidic Device (2018)
- 2D and 3D patterning of biological hydrogels for organ-on-chip applications (2018)
- Methacryl modified gelatin hydrogels for co-culture of cells to mimic blood-brain barrier (2018)
- Tailoring the biocompatibility of the elastomer PDMS for on-chip applications (2018)
- Improving the biocompatibility of PDMS by improving its curing time and temperature (2018)
- Towards the development of a microfluidic tool to assess the biological properties of biomaterials for bone regeneration (2018)
- Artificial spider silk production on a microfluidic chip (2018)
- Organs-on-Chip System with Integrated Transparent Conductive Oxide Electrodes (2018)
- Integrated transparent electrodes in an organs-on-chip system (2018)
- Chemical micropatterning of hyaluronic acid hydrogels for controlled cell adhesion (2018)
- Hyaluronic acid based hydrogel droplets: A potential injectable cell culture scaffold (2018)
- Production of hyaluronic acid-acrylamide microgels as potential cell culture scaffolds (2018)
- Integrating acoustophoresis to droplet microfluidics (2018)
- Introducing acoustophoresis to droplet microfluidics for particle manipulation (2018)
- Intra-droplet acoustic separation of two particle species in a droplet microfluidic system (2017)
- An optimized droplet split designed for acoustic intra-droplet particle enrichment (2017)
- A droplet unit operator for controlled particle switching and enrichment (2017)
- In situ temperature monitoring during acoustophoresis using integrated thin film Pt temperature sensors (2017)
- Internal temperature sensing in an acoustophoretic glass chip (2017)
- Using two-dimensional acoustophoresis for improved particle positioning in droplet microfluidics (2017)
- Addressing the biocompatibility of photo-crosslinkable hyaluronic acid hydrogels (2017)
- Assembly of a microfluidic device using only plasma activation (2017)
- Optimisation of a glass/PDMS/PDMS/glass microfluidic chip assembly using air plasma (2017)
- Acoustophoretic particle manipulation in droplet microfluidics at higher resonance modes (2016)
- Experimental investigation of resonance conditions for particle focusing in droplet acoustofluidics (2016)
- Particle enrichment in two-phase microfluidic systems using acoustophoresis (2016)
- Particle enrichment in droplet acoustofluidics (2016)
- Cross-linked gelatin/agarose conjugate as a thermostable and biocompatible microfluidic material (2016)
- Microstructured 3D cell culture scaffolds (2016)
- Microfluidic system with integrated 3D cell culture matrix (2016)
- Two-dimensional acoustic focusing of microparticles in two-phase droplet-based microfluidic systems increases particle detectability (2016)
- Two-dimensional acoustic focusing of microparticles in two-phase droplet-based microfluidic systems for improved particle positioning within spherical droplets (2016)
- 2D acoustophoretic positioning of microparticles inside droplets (2016)
- Fabrication of a silicon-glass microfluidic device for generation of sub 100-micrometer-sized droplets (2016)
- Aorta-on-a-chip as a tool to study the interface intima-media in the aortic wall (2016)
- Photopatterning of hyaluronic acid hydrogels for cell culture scaffolds (2016)
- Swelling properties of photocrosslinkable hyaluronic acid (2016)
- Spatial and chemical patterning of hyaluronic acid using UV lithography (2016)
- Focusing microparticles inside droplets using acoustics (2015)
- Acoustic focusing of microparticles in two-phase systems (2015)
- Micropatterning of photocrosslinkable hyaluronic acid (2015)
- Evaluation of a novel seeding technique in microfluidic chips (2015)
- Development of a droplet generator towards applications using acoustophoretic sorting (2014)
- Removal of proteins from blood using acoustophoresis (2014)
- Development of an electrochemical cantilever platform (2014)
- Characterization of Electromechanical Behavior of an Electrochemical Cantilever System (2014)
- Increased bead recovery using an acoustic trap as incubation site in multiplex assays (2014)
- Non-contact acoustic trapping platform for bead incubation for multiplex assays (2014)